163,545 results

1. pRK5-HA-Ubiquitin-K48

   Plasmid

   #17605

   Purpose

   Mammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to arginines

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K48 only, other lysines mutated to arginines. Enh…

   Promoter

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpyTag003-MBP

   Plasmid

   #133450

   Purpose

   Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-MBP
   

   Use

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Peptide tag added to N-terminus of MBP via a GSGE…

   Promoter

   T7

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-LAMP1-HaloTag

   Plasmid

   #164209

   Purpose

   Expresses HaloTag tagged LAMP1 under CMV promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGFP-D50 lamin A

   Plasmid

   #17653

   Depositor

   Tom Misteli

   Article

   Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

   Insert

   D50 lamin A (LMNA Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Delta 50 (50 aa internal deletion in globular tai…

   Promoter

   CMV

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. CROPseq-Guide-Puro

   Plasmid

   #86708

   Purpose

   Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

   Depositor

   Christoph Bock

   Article

   Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n

   Insert

   EF1a-Puro-WPRE-hU6-gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pArk313

   Plasmid

   #200257

   Purpose

   Ark313 rep/cap

   Depositor

   Justin Eyquem

   Article

   Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

   Insert

   Ark313 Rep/Cap
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-pAG-Hia5

   Plasmid

   #174373

   Purpose

   Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

   Depositor

   Aaron Straight

   Article

   Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

   Insert

   protein AG - hia5
   

   Use

   Tags

   6xHis and Protein A/G

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBb(RSF1010)1k-GFPuv

   Plasmid

   #106395

   Purpose

   IPTG-inducible trc promoter expressing gfpuv on RSF1010 origin

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSN837

   Plasmid

   #184825

   Purpose

   Expresses human KIF5A in mammalian cells

   Depositor

   Kyoko Chiba

   Article

   Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

   Insert

   KIF5A (KIF5A Human)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330S-2

    Plasmid

    #58778

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV500 empty vector

    Plasmid

    #33348

    Depositor

    Charles Vinson

    Article

    Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

    Type

    Empty backbone

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. gRNA_Cloning Vector

    Plasmid

    #41824

    Purpose

    An empty gRNA expression vector, used to create a gRNA to a specific sequence.

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    AflII site for Gibson Assembly

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-MAPTau-C-10

    Plasmid

    #55077

    Purpose

    Localization: Microtubules, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    MAPTau (MAPT Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. phL1A-pcDNA3

    Plasmid

    #12307

    Depositor

    Vance Lemmon

    Article

    Hlavin et al Genomics. 1991 Oct . 11(2):416-23.

    Insert

    L1CAM (L1CAM Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. hUbc13

    Plasmid

    #51131

    Purpose

    human ubc13, E2 for synthesis k63 linked ubiquitin chain

    Depositor

    Cynthia Wolberger

    Article

    Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

    Insert

    ubc13 (UBE2N Human)
    

    Use

    Tags

    His6 and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

16. FLAG-NLRC5

    Plasmid

    #37521

    Depositor

    Thomas Kufer

    Article

    Neerincx et al J Biol Chem. 2010 Aug 20;285(34):26223-3

    Insert

    NLRC5 (NLRC5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pwtCas9-bacteria

    Plasmid

    #44250

    Purpose

    aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    wild-type Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO-1

    Available Since

    April 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVUT-tTR-KRAB

    Plasmid

    #11651

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Has Service

    Cloning Grade DNA

    Insert

    hUbiquitin C, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TOMM20-mTurquoise-Rac1-G12V-deltaCaaX

    Plasmid

    #176124

    Purpose

    Constitutively active, mitochondrial localized Rac1

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    RAC1 (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX4T-E2-25K

    Plasmid

    #63572

    Purpose

    Expression of human Ubiquitin conjugating enzyme E2-25K

    Depositor

    Titia Sixma

    Article

    Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

    Insert

    UBE2-25K (UBA7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Isoform 1 (200 resiudes)

    Promoter

    tac

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only