165,688 results

1. FUGW-ER-TurboID

   Plasmid

   #200965

   Purpose

   3rd gen lentiviral plasmid for expressing endoplasmic reticulum localized TurboID with EGFP

   Depositor

   Keriann Backus

   Article

   Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

   Insert

   ER-TurboID
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223-CSNK1D

   Plasmid

   #23796

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   CSNK1D (CSNK1D Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pGL0_12 [pAMβ1 ORI]

   Plasmid

   #198926

   Purpose

   Level 0 part

   Depositor

   Cultivarium Tools, Nili Ostrov, Charlie Gilbert

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pAMβ1 ORI
   

   Use

   Synthetic Biology

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. BPK1520

   Plasmid

   #65777

   Purpose

   Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   SpCas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEG BacMam N term StrepII eGFP 3C

   Plasmid

   #160683

   Purpose

   Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

   Depositor

   Eric Gouaux

   Article

   Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

   Type

   Empty backbone

   Tags

   StrepII eGFP 3C

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOG44_FLP_recombinase (pEHA1338)

   Plasmid

   #209087

   Purpose

   FLP recombinase for FRT-mediated integration

   Depositor

   Vikram Khurana

   Article

   Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

   Insert

   FLP recombinase
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 HA human NEMO

   Plasmid

   #13512

   Depositor

   Kunliang Guan

   Article

   Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

   Insert

   NEMO (IKBKG Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Cyto-ExRai-AktAR2

   Plasmid

   #184048

   Purpose

   Cytosol-targeted ExRai-AktAR2.

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   Cyto-ExRai-AktAR2
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF1652

   Plasmid

   #143532

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SPI1 (SPI1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSP1830

    Plasmid

    #70708

    Purpose

    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Insert

    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    E782K, N968K and R1015H in SaCas9

    Promoter

    CAG

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICSL01009::AtU6p

    Plasmid

    #46968

    Purpose

    Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.

    Depositor

    Sophien Kamoun

    Article

    Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

    Insert

    AtU6p
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX_305_Ovalbumin

    Plasmid

    #184924

    Purpose

    Lentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.

    Depositor

    Arlene Sharpe

    Article

    Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

    Insert

    Ovalbumin (OVAL Chicken)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Our vector has an A188T mutation that does not im…

    Promoter

    PGK

    Available Since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. hSERT pcDNA3

    Plasmid

    #15483

    Depositor

    Randy Blakely

    Article

    Ramamoorthy et al Proc Natl Acad Sci U S A. 1993 Mar 15. 9

    Insert

    hSERT (SLC6A4 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET/ELP-I-CAT

    Plasmid

    #71461

    Purpose

    ELP-intein purification system

    Depositor

    David Wood

    Article

    Banki et al Nat Methods. 2005 Sep;2(9):659-61.

    Type

    Empty backbone

    Tags

    ELP ((VPGXG)110) and intein

    Expression

    Bacterial

    Available Since

    April 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVpuro-CMV-N-EGFP

    Plasmid

    #122848

    Purpose

    N-terminal EGFP tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIVT-Flp-2A-BleoR

    Plasmid

    #232752

    Purpose

    IVT template for making bicistronic modRNA encoding Flippase and Zeocin resistance gene

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Inserts

    FlpO
    BleoR
    

    Use

    In vitro transcription

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pICE-RNaseHI-D10R-E48R-NLS-mCherry

    Plasmid

    #60367

    Purpose

    Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Insert

    RNase HI-D10R-E48R
    

    Tags

    NLS from SV40 T antigen and mCherry

    Expression

    Mammalian

    Mutation

    D10R+E48R; catalyticaly inactive

    Promoter

    CMV-tet

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC19_CRISPR_DpmrA

    Plasmid

    #160903

    Purpose

    CRISPR_Cas9, lambda red recombineering

    Depositor

    Anne-Catrin Uhlemann

    Article

    McConville et al Cell Rep. 2020 Oct 27;33(4):108313. doi:

    Inserts

    Cas9
    Lambda Red Recombineering Genes
    Zeocin Resistance Cassette
    

    Promoter

    araBAD

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti6/V5/DEST-VPS35

    Plasmid

    #21691

    Depositor

    Lynda Chin

    Article

    Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

    Insert

    Vacuolar Protein Sorting 35 homolog (VPS35 Human)
    

    Use

    Lentiviral; Invitrogen gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAF0034 Ef1a-Kp03-2a-EGFP

    Plasmid

    #193461

    Purpose

    Expression of Kp03 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Kp03
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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