159,332 results

1. pDestTol2pA2-9xGRE

   Plasmid

   #74736

   Purpose

   GRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements

   Depositor

   Luisa Dalla Valle

   Article

   Benato et al Mol Cell Endocrinol. 2014 Jul 5;392(1-2)

   Insert

   EGFP
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   nine GC-responsive elements (9xGRE) from the rat …

   Available since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAcGP67A - murine TfR

   Plasmid

   #12392

   Depositor

   Pamela Bjorkman

   Insert

   transferrin receptor (Tfrc Mouse)
   

   Use

   Tags

   6X-His tag, Factor Xa cleavage site, and leader p…

   Expression

   Insect

   Mutation

   See comments section

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. Bxb1-GT_AIO-TetOn_Down-Tandem Donor

   Plasmid

   #229783

   Purpose

   Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   LacZ; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE and CAG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Bxb1-GA_AIO-TetOn_Down-Tandem Donor

   Plasmid

   #229785

   Purpose

   Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   LacZ; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE and CAG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSMART-HCKan-yibDp-VHH-VCAM1

   Plasmid

   #202471

   Purpose

   Expresses the nanobody VCAM1 after phosphate depletion

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   nanobody VCAM1
   

   Use

   Tags

   6X-His

   Expression

   Bacterial

   Mutation

   Promoter

   yibDp

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac His6 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-B)

   Plasmid

   #55219

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2T-CMV-EA-BE4max-NG-BlastR

   Plasmid

   #152992

   Purpose

   High purity C-to-T base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   BE4 H47E+S48A-nSpCas9-UGI-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRK-ATF4

   Plasmid

   #26114

   Depositor

   Yihong Ye

   Article

   Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

   Insert

   ATF4 (ATF4 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSEVA6213S

   Plasmid

   #122094

   Purpose

   constitutive expression of I-SceI for genome engineering in Pseudomonas

   Depositor

   Pablo Ivan Nikel

   Article

   Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

   Insert

   I-SceI restriction endonuclease
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Pem7

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHCMV_HCV_E1E2

    Plasmid

    #207279

    Purpose

    Mammalian expression of Hepatitis C virus E1E2 protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    HCV_E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTC217

    Plasmid

    #70018

    Purpose

    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b

    Depositor

    Daniel Voytas

    Article

    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

    Insert

    Nuclease (Cas9/sgRNA) + Donor + GVR
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. LDB-3-p65

    Plasmid

    #158984

    Purpose

    Expresses LDB-3 fused with a C-terminal p65 transcriptional activation domain in mammalian cells

    Depositor

    Liangcai Gu

    Article

    Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

    Insert

    LDB-3-p65
    

    Use

    Lentiviral

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. SIRT7.4

    Plasmid

    #13740

    Depositor

    John Denu

    Article

    Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

    Insert

    SIRT7 (SIRT7 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pLNCX2-mCherry-CHMP4B

    Plasmid

    #116923

    Purpose

    expresses mCherry-CHMP4B in mammalian cells

    Depositor

    Sanford Simon

    Article

    Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

    Insert

    CHMP4B (CHMP4B Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Thy1.1-GFP

    Plasmid

    #131703

    Purpose

    encodes Thy1.1 followed by a P2A site and GFP for enriching pure populations of transfected cells

    Depositor

    Britt Glaunsinger

    Article

    Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

    Insert

    Thy1.1-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GPR65-Tango

    Plasmid

    #66370

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR65 (GPR65 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pDEST-CMV mCherry-GFP-LC3B G120A

    Plasmid

    #123235

    Purpose

    Expresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Glycine 120 to Alanine

    Promoter

    CMV

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmTurquoise2-LAMP1

    Plasmid

    #110948

    Purpose

    Expresses LAMP1 fused to mTurquoise2

    Depositor

    Pablo Rivera-Fuentes

    Article

    Halabi et al Nat Commun. 2019 Mar 15;10(1):1232. doi:

    Insert

    Lysosomal-associated membrane protein 1 (LAMP1 Human)
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. psi-CHECK3

    Plasmid

    #136010

    Purpose

    psi-CHECK2 plasmid modified so both Renilla and Firefly luciferase contain introns

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-AspS

    Plasmid

    #159618

    Purpose

    Expression plasmid for AcpS phosphopantetheinyl transferase

    Depositor

    Jun Yin, Christopher T Walsh

    Article

    Zhou et al ACS Chem Biol. 2007 May 22;2(5):337-46.

    Insert

    AcpS
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge