165,688 results

1. pHR_SFFV-sig-mCherry-PDGFRTM-WPRE

   Plasmid

   #216668

   Purpose

   Expresses membrane-bound mCherry in mammalian cells

   Depositor

   Leonardo Morsut

   Insert

   mCherry
   

   Tags

   PDGFR Transmembrane Domain

   Expression

   Mammalian

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLPT107

   Plasmid

   #85525

   Purpose

   Repressilator with triple reporter

   Depositor

   Johan Paulsson

   Article

   Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

   Inserts

   mCfp
   mVenus
   tetR
   lacI
   cI
   mKate2
   

   Use

   Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Available Since

   April 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-His6-ERK2-MEK1_R4F_coexpression

   Plasmid

   #39212

   Depositor

   Melanie Cobb

   Article

   Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

   Inserts

   Mek1 R4F (MAP2K1 Human)
   Erk2 (Mapk1 Rat)
   

   Tags

   His6 and RBS

   Expression

   Bacterial

   Mutation

   MEK1(R4F) = S218E, S222D and deletion of AA32-51

   Promoter

   T7 and T7 via RBS

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-CAG-rtTA-IRES-Hygro

   Plasmid

   #102423

   Purpose

   Mammalian expression vector for tetracycline-transactivator (rtTA), IRES-Hygro

   Depositor

   Kian Peng Koh

   Article

   Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

   Insert

   tetracyclin-transactivator (rtTA)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. RGB-S reporter

   Plasmid

   #207841

   Purpose

   A three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coli

   Depositor

   Christof Niemeyer

   Article

   Zoheir et al NPJ Biofilms Microbiomes. 2023 Aug 21;9(

   Inserts

   RpoH sensing construct
   SOS sensing construct
   RpoS sensing construct
   

   Expression

   Bacterial

   Promoter

   PosmY, PsulA, and grpE

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tet1-dCas9

   Plasmid

   #136650

   Purpose

   Tet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

   Insert

   Tet1 (Tet1 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-Tet1-dCas9-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP::L4440

   Plasmid

   #11335

   Depositor

   Guy Caldwell

   Insert

   GFP
   

   Use

   RNAi

   Expression

   Worm

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEG BacMam C term 3C eGFP StrepII

   Plasmid

   #160686

   Purpose

   Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

   Depositor

   Eric Gouaux

   Article

   Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

   Type

   Empty backbone

   Tags

   3C eGFP StrepII

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEVOL-AckRS

   Plasmid

   #137976

   Purpose

   sequence optimized N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion

   Depositor

   Wenshe Liu

   Article

   Wang et al J Am Chem Soc. 2019 Feb 13;141(6):2462-2

   Insert

   AckRS and pylTcua
   

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

    Plasmid

    #185919

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. UnReg-H2B-YFP-DD

    Plasmid

    #96892

    Purpose

    Reporter to monitor translation of unstructured mRNA

    Depositor

    Tobias Meyer

    Article

    Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

    Insert

    UnReg-H2B-Venus-DD
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWZL hygro Flag HA TRAP220 wt

    Plasmid

    #17433

    Purpose

    Retroviral expression of Med1/TRAP220 in mammalian cells

    Depositor

    Kai Ge

    Article

    Ge et al Mol Cell Biol. 2008 Feb . 28(3):1081-91.

    Insert

    TRAP220 (MED1 Human)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

    Plasmid

    #168290

    Purpose

    CRISPR-activator Suntag-P65-HSF1 (SPH)

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    dCas9, Suntag-P65-HSF1
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pICE-RNaseHI-WT-NLS-mCherry

    Plasmid

    #60365

    Purpose

    Plasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Insert

    RNase HI
    

    Tags

    NLS from SV40 T antigen and mCherry

    Expression

    Mammalian

    Promoter

    CMV-tet

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti_EFS-Cas9-P2A-HygR

    Plasmid

    #164134

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by EFS promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPGK-T7/2-CD44v9 (fl)

    Plasmid

    #137818

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v9 (fl) (CD44 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    full length and mutation H48L

    Promoter

    PGK

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-PM-ExRai-AktAR2

    Plasmid

    #184050

    Purpose

    Plasma membrane-targeted ExRai-AktAR2

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    PM-ExRai-AktAR2
    

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-WASH1-C-18

    Plasmid

    #55162

    Purpose

    Localization: Cytoskeleton, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    WASH1
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

19. GFP-Rab7A

    Plasmid

    #61803

    Purpose

    Human Rab7A fluorescently tagged with GFP on the N-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

    Insert

    Rab7A (RAB7A Human)
    

    Tags

    2x HA and GFP

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICH41780

    Plasmid

    #48019

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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