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We narrowed to 20 results for: BFP

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Showing: 1 - 20 of 20 results

  1. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...We began distributing ready-to-use virus preps through our viral service in late 2016 and requests are... any Cre-controlled gene of interest makes pmSyn-BFP-Cre highly modular. Further uses of pmSyn1-EBFP-Cre...

  2. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...GFP reporter that is converted into BFP (blue fluorescent protein) upon CRISPR/Cas9 homology directed ...Targeting pEF-BFP with a cytidine deaminase base editor results in shift in emission spectra from BFP to GFP....293 cells with the BFP variant, a BE4am-Cas9 base editor, and sgRNA vectors for the BFP-to-GFP conversion...Glasser et al., 2016). For TREE they engineered a BFP variant that undergoes conversion to GFP after being...cytidine deaminase base DNA editor. Specifically the BFP mutant (BFPH66) contains a histidine at amino acid...from the Wang and Brafman lab uses an engineered BFP variant that converts to GFP after a base editing...nature24644 Glaser A, McColl B, Vadolas J (2016) GFP to BFP Conversion: A Versatile Assay for the Quantification...

  3. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ... want to design a genetic FLEx switch that turns BFP expression off, while turning on mCherry expression...successfully work, the cassette would need to contain the BFP coding sequence in the sense orientation, followed...Nat Biotech 2003): Basic FLEx switch will express BFP in the absence of, or mCherry in the presence of ...specifically drives expression of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx useful...

  5. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ... range).  By mutating GFP, the variants blue FP (BFP), cyan FP (CFP), and yellow FP (YFP) were derived...will pass blue light to the detector/camera, then BFPs are of no use to you. When using more than one FP...

  7. Control AAV Preps

    Type
    Collection
    ...Ian Wickersham 137130 pAAV-Ef1a-Con/Foff 2.0-BFP EF1a BFP Cre dependent 8 Karl Deisseroth 137133 pAAV-... Viviana Gradinaru 137131 pAAV-Ef1a-Coff/Fon-BFP EF1a BFP Flp dependent 8 Karl Deisseroth 137134 pAAV-...rg* Karl Deisseroth 137129 pAAV-Ef1a-Con/Fon-BFP EF1a BFP Cre and Flp dependent 8 Karl Deisseroth 137132...

  8. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... outer membrane MFF AcGFP1 Gia Voeltz 49151 mito-BFP Mitochondria Mitochondrial targeting signal (COX4... Endosomes (early) Rab5 mCherry Gia Voeltz 49147 BFP-Rab5 Endosomes (early) Rab5 TagBFP Gia Voeltz 61802...Endosomes (early) Rab5a TagRFP James Johnson 79801 pTag-BFP-C-h-Rab5a-c-Myc Endosomes (early) Rab5a TagBFP James...reticulum ER retention signal oxBFP Erik Snapp 49150 BFP-KDEL Endoplasmic reticulum BiP ER signal sequence...Endoplasmic reticulum Sec61 mCherry Gia Voeltz 49154 BFP-Sec61 beta Endoplasmic reticulum Sec61 TagBFP Gia...recycling) Rab11a TagRFP James Johnson 79805 pTag-BFP-C-h-Rab11a-c-Myc Endosomes (recycling) Rab11a TagBFP...recycling) Rab4a TagRFP James Johnson 79799 pTag-BFP-C-h-Rab4a-c-Myc Endosomes (recycling) Rab4a TagBFP...

  10. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 WT] TARDBP CMV ALS Rajat Rohatgi 107840 pHBS1108 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43...IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 ∆CTD] TARDBP CMV ALS Rajat Rohatgi 107843 pHBS1185 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43...IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 2xsigma] TARDBP CMV ALS Rajat Rohatgi 107845 pHBS1202 [IBB-GFP-mCherry3E]-[BFP-P2A-...IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 6xsigma] TARDBP CMV ALS Rajat Rohatgi 107847 pHBS1187 [IBB-GFP-mCherry3E]-[BFP-P2A-...IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G295S] TARDBP CMV ALS Rajat Rohatgi 107849 pHBS1337 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43...IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G348S] TARDBP CMV ALS Rajat Rohatgi 107851 pHBS1285 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43...IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G348V] TARDBP CMV ALS Rajat Rohatgi 107854 pHBS1331 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43...

  11. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...pDAS12222_U6-pegRNA-BFP Mammalian hU6 pegRNA BpiI for pegRNA, Esp31 for PBS-RT No BFP Ervin Welker Return...

  13. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...Structure Plasmids: ER mCherry-Sec61 beta BFP-Sec61 beta BFP-KDEL Rtn4a-GFP (tubular ER) Microtubules...-Rab5 BFP-Rab5 GFP-Rab5B Vacuolar & Budding Compartment GFP-Rab4B Mitochondria mito-BFP mCherry-Drp1...dCas9s with one of three fluorescent proteins (GFP, BFP and mCherry), the authors were able to visualize ...

  15. Deisseroth INTRSECT Collection

    Type
    Collection
    ...137129 pAAV-Ef1a-Con/Fon-BFP Cre AND Flp Yes 137130 pAAV-Ef1a-Con/Foff 2.0-BFP Cre AND NOT Flp FRT/F5 Yes... Yes 137131 pAAV-Ef1a-Coff/Fon-BFP Flp AND NOT Cre Yes 137132 pAAV-Ef1a-Con/Fon-mCherry Cre AND Flp Yes...
Showing: 1 - 20 of 20 results