We narrowed to 14 results for: BFP

...We began distributing ready-to-use virus preps through our viral service in late 2016 and requests are... any Cre-controlled gene of interest makes pmSyn-BFP-Cre highly modular. Further uses of pmSyn1-EBFP-Cre...

...GFP reporter that is converted into BFP (blue fluorescent protein) upon CRISPR/Cas9 homology directed ...Targeting pEF-BFP with a cytidine deaminase base editor results in shift in emission spectra from BFP to GFP....293 cells with the BFP variant, a BE4am-Cas9 base editor, and sgRNA vectors for the BFP-to-GFP conversion...Glasser et al., 2016). For TREE they engineered a BFP variant that undergoes conversion to GFP after being...cytidine deaminase base DNA editor. Specifically the BFP mutant (BFPH66) contains a histidine at amino acid...from the Wang and Brafman lab uses an engineered BFP variant that converts to GFP after a base editing...nature24644 Glaser A, McColl B, Vadolas J (2016) GFP to BFP Conversion: A Versatile Assay for the Quantification...

... want to design a genetic FLEx switch that turns BFP expression off, while turning on mCherry expression...successfully work, the cassette would need to contain the BFP coding sequence in the sense orientation, followed...Nat Biotech 2003): Basic FLEx switch will express BFP in the absence of, or mCherry in the presence of ...specifically drives expression of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx useful...

...nanobody cross-reacts with BFP, sender cells expressing surface BFP generated BFP-labeled receiver cells....

... range).  By mutating GFP, the variants blue FP (BFP), cyan FP (CFP), and yellow FP (YFP) were derived...will pass blue light to the detector/camera, then BFPs are of no use to you. When using more than one FP...

...FACS, gating for the top quartile (or higher) of BFP and GFP producing cells. After FACS, return the cells...cotransfect a mixture of 1248 ng of CMV-gvpA-IRES-BFP plasmid and 954 ng CMV-gvpNJKFGWV plasmid into 70%...

...hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain two SfiI restriction...

...PubMed Central PMCID: PMC5495063 Kan et al. use a GFP-BFP conversion system to assess precision genome editing...

...Structure Plasmids: ER mCherry-Sec61 beta BFP-Sec61 beta BFP-KDEL Rtn4a-GFP (tubular ER) Microtubules...-Rab5 BFP-Rab5 GFP-Rab5B Vacuolar & Budding Compartment GFP-Rab4B Mitochondria mito-BFP mCherry-Drp1...dCas9s with one of three fluorescent proteins (GFP, BFP and mCherry), the authors were able to visualize ...

...that each recruits a different fluorescent protein (BFP, GFP, or RFP) to the genomic loci of interest. Pairs...

...hairpin sequences, which recruit fluorescent proteins BFP, GFP, and RFP fused to the respective hairpin binding...

... family members (superfolder, Emerald, CFP, YFP, BFP, Cerulean, Turquoise, Venus, and Citrine) can be ...

...Cysteineless non-glycosylated monomeric blue fluorescent protein, secBFP2, for studies in the eukaryotic...

...derivatives) Y145F Increases quantum yield for BFP Y145A and H148D Stabilizes the structure of Cerulean...