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We narrowed to 9,801 results for: pET

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Showing: 1701 - 1720 of 9801 results

  15. pET-FLAG-B-eSpCas9

    Plasmid
    #126772
    Purpose
    Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    T7
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126772-plasmid-map-sequence-id-410600

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  19. pET-30a(+)-C-SH2+IA

    Plasmid
    #111418
    Purpose
    express murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Protein Sci. 2018 Jul 27. doi: 10.1002/p
    Insert
    murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), without any tag (Syk Mouse)
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111418-plasmid-map-sequence-id-215713

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  20. pET-30a(+)-N-SH2

    Plasmid
    #111274
    Purpose
    express murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
    Tags
    HHHHHHENLYFQG
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111274-plasmid-map-sequence-id-215709

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Showing: 1701 - 1720 of 9801 results