We narrowed to 15,745 results for: pET

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pET28 His6-mEGFP-D4

Plasmid

#226372

Purpose

Production of recombinant mEGFP-D4, a fluorescent probe binding cholesterol

Depositor

Catherine Tomasetto

Article

Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

Insert

D4 (pfoA Clostridium perfringens)

Tags

His6 and monomeric EGFP

Expression

Bacterial

Promoter

T7

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28a_6H-TAQ_E602D

Plasmid

#166944

Purpose

Bacterial expression of Taq DNA polymerase

Depositor

Robert Tjian

Article

Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

Insert

Thermus aquaticus DNA polymerase

Tags

6X His

Expression

Bacterial

Mutation

E602D

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b-hUba2-His

Plasmid

#53117

Purpose

expression of full length human Uba2 with Cterminal His-tag in E.coli

Depositor

Frauke Melchior

Article

Werner et al Methods Mol Biol. 2009;497:187-99. doi:

Insert

UBA2 (UBA2 Human)

Tags

His and T7 tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-16

Plasmid

#172746

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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BARD1 26-327 pET28a

Plasmid

#12646

Depositor

Rachel Klevit

Article

Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

Insert

BRCA1 binding partner for a Ring domain E3 Ub-ligase (BARD1 Human)

Expression

Bacterial

Available Since

July 31, 2007

Availability

Academic Institutions and Nonprofits only

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pET28a-His-hAos1

Plasmid

#53135

Purpose

E.coli expression of full length human Aos1 (SAE1) with Nterminal His-tag

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

Aos1 (SAE1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-A762V

Plasmid

#174795

Purpose

Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A762V (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

A762V

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-EGFP-CNA35

Plasmid

#61603

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with EGFP

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

EGFP and CNA35

Tags

His tags

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pET-pAG-Hia5

Plasmid

#174373

Purpose

Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

Depositor

Aaron Straight

Article

Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

Insert

protein AG - hia5

Tags

6xHis and Protein A/G

Expression

Bacterial

Available Since

Sept. 6, 2021

Availability

Academic Institutions and Nonprofits only

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BirA in pET28a (w400-2)

Plasmid

#26624

Depositor

Eric Campeau

Insert

BirA

Tags

His

Expression

Bacterial

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaD (pESC106)

Plasmid

#50388

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

Depositor

Tadhg Begley, Erick Strauss

Article

Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

Insert

PPAT

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-15

Plasmid

#228971

Purpose

For bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-15

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET41b+_Ufd1-His

Plasmid

#117107

Purpose

Production of recombinant Ufd1-His in E.coli

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

Ufd1 (UFD1 Human)

Tags

His

Expression

Bacterial

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaE (pESC124)

Plasmid

#50390

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.

Depositor

Tadhg Begley, Erick Strauss

Article

Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

Insert

DPCK

Tags

6XHIS tag

Expression

Bacterial

Promoter

T7

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pET21a-alpha-synuclein

Plasmid

#51486

Purpose

Inducible expression of human alpha-synuclein in E.coli

Depositor

Michael J Fox Foundation MJFF

Insert

alpha-synuclein (SNCA Human)

Expression

Bacterial

Promoter

T7

Available Since

March 31, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pET21-pelB-LaG94-10

Plasmid

#228969

Purpose

For bacterial expression of anti-GFP nanobody LaG94-10, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-10

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-FGF2-G3

Plasmid

#135521

Purpose

Engineered form of fibroblast growth factor 2 with improved thermostability

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

fibroblast growth factor 2 (FGF2 Human)

Tags

6x His tag and Agg Thrombin Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promoter

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28a_6H-MMLV_RT_D524N-6H

Plasmid

#166945

Purpose

Bacterial expression of M-MLV reverse transcriptase

Depositor

Robert Tjian

Article

Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

Insert

gag-pol

Tags

6X His

Expression

Bacterial

Mutation

D524N, H8Y

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET263-pUC57 24xMS2V7

Plasmid

#140705

Purpose

MS2V7 cloning

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V7

Use

Cloning vector

Mutation

24 MS2 loops V7, high-affinity C-variant, inter-s…

Promoter

None

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK522-FAST-PETase

Plasmid

#214754

Purpose

plasmid for expression FAST-PETase in Pseudomonas putida

Depositor

Hal Alper

Article

Lu et al Nature. 2022 Apr;604(7907):662-667. doi:

Insert

FAST-PETase

Expression

Bacterial

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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