We narrowed to 3,447 results for: zebrafish

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pMC-Dest-uni-gRNA5-BsaI

Plasmid

#241150

Purpose

Destination vector for mini-Golden subcloning platform; contains universal sgRNA at 5' end

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Type

Empty backbone

Use

Minicircle cloning vector

Mutation

TCGC linker

Available since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_ect2_cmlc2-nmKate

Plasmid

#238410

Purpose

Drives expression of 3 different gRNAs targeting ect2, and expression of nuclear mKate in cardiomyocytes

Depositor

Juan Manuel González-Rosa

Publication

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting ect2

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_tnnt2a_cmlc2-nmKate

Plasmid

#238309

Purpose

Drives expression of 3 different gRNAs targeting tnnt2a, and expression of nuclear mKate in cardiomyocytes.

Depositor

Juan Manuel González-Rosa

Publication

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting tnnt2a

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_erbb2_cmlc2-nmKate

Plasmid

#238409

Purpose

Drives expression of 3 different gRNAs targeting erbb2, and expression of nuclear mKate in cardiomyocytes

Depositor

Juan Manuel González-Rosa

Publication

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting tnnt2a

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_amhc(myh6)_cmlc2-nmKate

Plasmid

#238375

Purpose

Drives expression of 3 different gRNAs targeting amhc (myh6), and expression of nclear mKate in cardiomyocytes.

Depositor

Juan Manuel González-Rosa

Publication

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting amhc

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME mCherry-P2A-NTRv2-pA

Plasmid

#200532

Purpose

mini circle middle entry plasmid for mCherry- P2A- ntr version 2- pA with flanking BsaI cut sites

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry, NTRv2

Use

Mini-golden

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pet28bZFCNGA5

Plasmid

#69858

Purpose

His tagged protein for antibody production screening

Depositor

Gary Matthews

Publication

Khan et al Neuroscience. 2010 Jan 13;165(1):79-89.

Insert

ZFCNGA5, 3' end only with native stop (cnga5 Zebrafish)

Tags

His

Expression

Bacterial

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pT3TS-RfxCas13d-2C-NLS

Plasmid

#239816

Purpose

Plasmid to carry out IVT of RfxCas13d with SV40 and nucleoplasmin long-NLP NLS (human codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Publication

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

Rfx-Cas13d

Use

CRISPR

Tags

Nucleoplasmin long NLS and SV40 NLS

Available since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pT3TS-RfxCas13d-hei

Plasmid

#234485

Purpose

Plasmid to carry out IVT of RfxCas13d with myc tag and two oNLS (human codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Publication

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

RfxCas13d

Use

CRISPR

Tags

myc and oNLS

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF

Plasmid

#119864

Purpose

Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

Depositor

Tatjana Sauka-Spengler

Publication

Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

Insert

BirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene

Tags

HA-tagged biotin ligase (BirA), Thosea asigna vir…

Expression

Bacterial

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM-T-Easy-NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT

Plasmid

#119067

Purpose

Donor construct to amplify NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

Depositor

Tatjana Sauka-Spengler

Publication

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT

Expression

Bacterial

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-TagRFP-gcry-eGFP

Plasmid

#117767

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Publication

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGTag-eGFP-CAAX-B-actin

Plasmid

#117816

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Publication

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Tags

CAAX

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pME-pACre

Plasmid

#226789

Purpose

Middle-entry vector with photoactivatable Cre recombinase (pACre). pACre activated with 470nm Blue Light. For use in Gateway cloning.

Depositor

Florence Marlow

Publication

Pahima et al Development. 2026 Jul 6:dev.204904. doi:

Insert

pACre (Photoactivatable Cre)

Use

Cre/Lox; Gateway cloning middle-entry vector

Available since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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pCSDest2_pACre-nos3'utr

Plasmid

#226790

Purpose

Photoactivatable Cre recombinase (pACre) with the nanos3,3'utr for PGC-specificity. Vector used to transcribe mRNA for injection. pACre activated with 470nm Blue Light.

Depositor

Florence Marlow

Publication

Pahima et al Development. 2026 Jul 6:dev.204904. doi:

Inserts

pACre (Photoactivatable Cre)
nanos3, 3'utr

Use

Cre/Lox; For ivt

Available since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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pGEM-T_hmx2

Plasmid

#254516

Purpose

Partial hmx2 cDNA. For in vitro transcription of ISH probe

Depositor

Virginie Lecaudey

Publication

Lardennois et al bioRxiv 2025.05.08.652796

Insert

hmx2 (hmx2 Zebrafish)

Use

Zebrafish expression

Promoter

T7

Available since

June 25, 2026

Availability

Academic Institutions and Nonprofits only

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pGEM-T_vgll4l

Plasmid

#254515

Purpose

Partial vgll4l cDNA. For in vitro transcription of ISH probe

Depositor

Virginie Lecaudey

Publication

Lardennois et al bioRxiv 2025.05.08.652796

Insert

vgll4l (vgll4l Zebrafish)

Use

Zebrafish expression

Promoter

SP6

Available since

June 25, 2026

Availability

Academic Institutions and Nonprofits only

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hsp70I: cPla-mKate2-P2A-EGFP-KDEL

Plasmid

#250915

Purpose

Dual color expression of mKate2 tagged zebrafish cPla2 and ER markers (EGFP-KDEL) in zebrafish live tissue after heat shock

Depositor

Philipp Niethammer

Publication

Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/

Insert

cPla2-mKate2-P2A-EGFP-KDEL (pla2g4aa Zebrafish)

Use

D.rerio (zebrafish)

Tags

KDEL on EGFP and mKate2 on cPla2

Promoter

hsp70i

Available since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV-EGFPCAAX

Plasmid

#232140

Purpose

Expresses EGFP with a CAAX motif under control of a CMV promoter

Depositor

Steven Farber

Publication

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

EGFPCAAX

Use

Zebrafish expression

Promoter

CMV

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-drAgo2

Plasmid

#221711

Purpose

Expression of zebrafish Ago2 in zebrafish embryos.

Depositor

David Bartel

Publication

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Use

Fish expression

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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