We narrowed to 4,734 results for: Drosophila

1. pC5Kan-P2A

   Plasmid

   #51814

   Purpose

   P2A peptide from porcine teschovirus-1 flanked by restriction sites in shuttle vector for easy cloning of multiple separate proteins to be expressed from single message

   Depositor

   Barry Ganetzky

   Article

   Daniels et al PLoS One. 2014 Jun 19;9(6):e100637. doi:

   Insert

   P2A peptide (PTV1gp1 Synthetic, porcine teschovirus-1)
   

   Use

   Shuttle vector for insect expression vectors

   Mutation

   codons altered for Drosophila and to eliminate re…

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABR IRFP-Bcd-LEXY

   Plasmid

   #182595

   Purpose

   Drosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.

   Depositor

   Jared Toettcher

   Article

   Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

   Insert

   Bcd (bcd Fly)
   

   Tags

   IRFP and LEXY

   Expression

   Insect

   Promoter

   maternal tubulin

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUWR-CadTS-C

   Plasmid

   #58326

   Purpose

   Destination vector for inserting ubi-CadTS control to Drosophila melanogaster genome

   Depositor

   Denise Montell

   Article

   Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

   Insert

   E-cadherin tension sensor control
   

   Expression

   Insect

   Promoter

   poly-ubiquitin

   Available Since

   Aug. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pAFW-Ago1

   Plasmid

   #50553

   Purpose

   Expression of FLAG tagged Ago1 in S2 cell

   Depositor

   Yukihide Tomari

   Article

   Kawamata et al Nat Struct Mol Biol. 2009 Sep;16(9):953-

   Insert

   Argonaute1 (AGO1 Fly)
   

   Tags

   FLAG

   Expression

   Insect

   Promoter

   Actin5C

   Available Since

   Feb. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPaint-T2A-Cas9-T2A-GFP-3xP3-RFP

   Plasmid

   #127560

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Cas9-T2A-GFP-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   Cas9-T2A-GFP
   

   Use

   CRISPR

   Expression

   Insect

   Mutation

   EGFP-D102G see depositor comments below

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc5.1B-lambdaN-HA-DmAGO1

   Plasmid

   #22420

   Depositor

   Elisa Izaurralde

   Article

   Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

   Insert

   Argonaut-1, variant A (AGO1 Fly)
   

   Use

   Drosophila expression

   Tags

   lambdaN-HA

   Available Since

   Nov. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28b Me31B-3Myc

   Plasmid

   #113001

   Purpose

   For protein expression and purification of Myc-tagged full-length Drosophila me31B

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   full length me31B (me31B Fly)
   

   Tags

   Myc

   Expression

   Bacterial

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUAS-CFP-MEK

   Plasmid

   #42077

   Depositor

   Norbert Perrimon

   Article

   Friedman et al Nature. 2006 Nov 9;444(7116):230-4. Epub

   Insert

   Drosophila MEK ortholog (Dsor1 Fly)
   

   Tags

   CFP

   Expression

   Insect

   Promoter

   Hsp70

   Available Since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pGex4t2 with full-length SUUR

   Plasmid

   #112990

   Purpose

   Expresses fusion of GST and full-length Drosophila SUUR

   Depositor

   Terry Orr-Weaver

   Article

   Nordman et al Cell Rep. 2014 Nov 6;9(3):841-9. doi: 10

   Insert

   SuUR (SuUR Fly)
   

   Expression

   Bacterial

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUAST attB Orco (Y478A)

    Plasmid

    #73928

    Purpose

    UAS induced expression of Drosophila melanogaster Orco cDNA with 478 Tyrosine to Alanine amino acid change

    Depositor

    Leslie Vosshall

    Article

    Nakagawa et al PLoS One. 2012;7(3):e32372. doi: 10.1371

    Insert

    Orco (Y478A) (Orco Fly)
    

    Use

    Unspecified

    Mutation

    changed 478 Tyrosine for Alanine

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Hy_pMT Laccase2 MCS-ZKSCAN1 548-1047

    Plasmid

    #69886

    Purpose

    Expresses a miniature version of the ZKSCAN1 circular RNA in Drosophila

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1 (stw, ZKSCAN1 Human)
    

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXS-IRES-Blast CG1358

    Plasmid

    #218541

    Purpose

    Drosophila CG1358 cDNA (human codon optimized)

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    CG1358 (HisT Fly)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAFMW-Nbr[R280A,K284A]

    Plasmid

    #188598

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    R280A [CGA=>GCA], K284A [AAG=> GCG]

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAFMW-Nbr[C399-625 CM]

    Plasmid

    #188588

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler C-terminal domain, catalytic mutant
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    D435A [GAT=>GCC]; E435A [GAA=>GCC]

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAFMW-Nbr[C399-625]-Gwpep(Mm)

    Plasmid

    #188589

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    GW-peptide (mouse) C-terminally tagged C-terminal domain of Nibbler
    

    Tags

    FLAG-Myc and GWpep mm

    Expression

    Insect

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAFMW-Gwpep(Mm)-Nbr[C399-625]

    Plasmid

    #188591

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    GW-peptide (mouse) N-terminally tagged C-terminal domain of Nibbler
    

    Tags

    FLAG-Myc-Gwpep mm

    Expression

    Insect

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAFMW-Nbr[R272A,K276A]

    Plasmid

    #188596

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    R272A [CGG=> GCG], K276A [AAG=>GCG]

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBABR mCherry-Bcd-LEXY

    Plasmid

    #182596

    Purpose

    Drosophila integration plasmid expressing mCherry-Bcd-LEXY fusion protein under the maternal tubulin promoter.

    Depositor

    Jared Toettcher

    Article

    Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

    Insert

    Bcd (bcd Fly)
    

    Tags

    LEXY and mCherry

    Expression

    Insect

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-4T-1-Staufen-dsRBD(+6)

    Plasmid

    #171546

    Purpose

    Encoding part of the Drosophila Staufen protein from M571 to P656

    Depositor

    Yan Zeng

    Article

    Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    Staufen (stau Fly)
    

    Expression

    Bacterial

    Mutation

    a six amino acid_TRLTEG_insertion in the L1 region

    Available Since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX6P-1 BICC FL

    Plasmid

    #112998

    Purpose

    For protein expression and purification of full-length Drosophila BicC

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length BicC (BicC Fly)
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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