We narrowed to 29 results for: broad host range

Looking for a plasmid that can thrive in a number of different bacteria? Learn about broad host range plasmids in this post!

There are many tools available for studying the microbiome. Learn about plasmid tools for engineering gut microbes, detection, and synthetic biology.

This post describes the design of shuttle vectors, which are designed for replication, selection, and expression in more than one host species.

This post describes applications of pooled libraries, briefly explains several approaches, and highlights the most popular pooled libraries at Addgene.

Designing a CRISPR experiment in bacteira? Check out these tools for genome engineering, CRISPRa, CRISPRi, base editing, and microbiome engineering.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Looking to use adenoviral vectors in your research? This post introduces you to the adenovius!

Lentiviral transductions can be frustrating. Follow these tips to achieve consistently successful lentiviral transductions.

Addgene answers questions about lentiviral plasmids, including 2nd vs 3rd generation transfer vectors, packaging plasmids, biosafety concerns, and more.

Browse Addgene’s curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community forums.

Pseudotyping is a method use to introduce a viral envelope protein from another virus to restrict or broaden host cell. Get the what, why, how of pseudotyping in this article.

Viral research basics and their use as biological tools. Learn about retrovirus, lentivirus, adenovirus, and AAV.

In this blog post, we explain gamma-retrovirus packaging systems for those interested in using gamma-retroviral systems in the lab.

Laura Drepanos from the Broad Institute shares the strategies her team used to optimize the CRISPR knockout libraries Jacquere and Julianna.

Learn ways you can bring your science to elementary and middle school by hosting events and giving talks geared towards elementary and middle school students.

The third post in the mentoring series, this post describes ways you can approach finding a science mentor and advice on how to “make the ask.”

We describe the different classes of optogenetic tools available and what levels of control they give you in your experimental setup.

Addgene's protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting human and mouse genes.

Learn how and why Adeno-associated virus (AAV) has enabled breakthroughs in retinal gene therapy.

Learn how to target donor and acceptor fluorophores for FRET experiments.