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We narrowed to 45 results for: nls

Showing: 1 - 20 of 45 results

  1. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...region of a reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) an RFP protein that binds to the 3’ UTR ...UTR of the reporter mRNA via an MS2 coat protein (NLS-MS2-RFP) the reporter mRNA, which contains the tethering... to appear yellow. The act of translation pushes NLS-PCP-GFP off of the mRNA, leaving only a red FP bound...Because it contains a nuclear localization signal, NLS-PCP-GFP then returns to the nucleus to bind cognate...allow continued tracking of a given mRNA because NLS-PCP-GFP is removed by the first ribosome that translates...

  2. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...pAAV-FLEX-tdTomato pAAV-GFAP104-mCherry pAAV-mDlx-NLS-mRuby2 CAG-NLS-GFP pAAV-hSyn-mCherry Read on to learn ...pAAV-FLEX-tdTomato, pAAV-GFAP104-mCherry, pAAV-mDlx-NLS-mRuby2, CAG-NLS-GFP,and pAAV-hSyn-mCherry at Addgene. Viral...

  3. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...62933 pET-Cas9-NLS-6xHis 6xHis (C terminal); NLS (C terminal) 62934 pET-NLS-Cas9-6xHis 6xHis...(N terminal); 2xNLS (C terminal) 47327 pET-28b-Cas9-His 6xHis (C terminal); NLS (C terminal) ...6xHis (C terminal); NLS (N terminal)   Easi-CRISPR efficiency and delivery methods Using RNP delivery...

  4. New Viral Vectors - March 2024

    Type
    Blog Post
    ...AAV9 Biosensor Looger New serotype pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST AAV9 Optogenetics Adesnik ...7 AAV2 Controls Fishell New serotype pAAV-mDlx-NLS-mRuby2  AAV PHP.eB Controls Gradinaru New serotype...

  5. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...nuclear localization (LINuS). Blue light renders the NLS accessible and the complex enters the nucleus where...Mxi1 can repress gene expression. In the dark, the NLS is inaccessible and the complex remains in the cytoplasm...

  7. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...activation. Multiple nuclear localization signals (NLS) increase protein import into the nucleus. In developing...

  10. The Pleiades Promoter Project

    Type
    Collection
    ...EGFP/NLS Ple11 AVP pEMS1240 EGFP/NLS Ple12 AVP pEMS1241 EGFP/NLS Ple12 AVP pEMS1482 intron-lacZ/NLS Ple13... EGFP/NLS Ple38 CRH pEMS1155 EGFP/NLS Ple39 CRH pEMS1156 EGFP/NLS Ple44 CX3CR1 pEMS1149 EGFP/NLS Ple45...EGFP/NLS Ple52 DCX pEMS1198 EGFP/NLS Ple53 DCX pEMS1199 EGFP/NLS Ple53 DCX pEMS1524 intron-lacZ/NLS Ple54...EGFP/NLS Ple55 DCX pEMS1526 intron-lacZ/NLS Ple56 DDC pEMS1125 EGFP/NLS Ple57 DDC pEMS1126 EGFP/NLS Ple58...intron-lacZ/NLS Ple62 DRD1 pEMS1167 EGFP/NLS Ple63 DRD1 pEMS1168 EGFP/NLS Ple64 FEV pEMS1129 EGFP/cre/NLS Ple64...-lacZ/NLS Ple80 GABRA6 pEMS1434 EGFP/NLS Ple81 GAL pEMS1177 EGFP/NLS Ple84 GAL pEMS1180 EGFP/NLS Ple85.../cre/NLS Ple85 GCHFR pEMS1414 EGFP/NLS Ple87 GCHFR pEMS1416 EGFP/NLS Ple88 GFAP pEMS1375 EGFP/NLS Ple88...

  14. Control AAV Preps

    Type
    Collection
    ...8, 9, rg*, PHP.eB Fishell 99130 pAAV-mDlx-NLS-mRuby2 Dlx NLS-mRuby2 Constitutive 1, 2, 8, 9, rg*, PHP....Constitutive 2, 5, rg*, PHP.eB Gradinaru 104061 CAG-NLS-GFP CAG NLS-GFP Constitutive PHPeB Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH...pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a NLS-mCherry or nls-EGFP Cre dependent 1, 2, 5, 8, 9, rg* Harvey...Constitutive 1, 8 Deisseroth 135630 pAAV-S5E2-dTom-nlsdTom E2 regulatory dTomato Constitutive 1, 9, PHP.eB...EF1a mito-GFP Cre dependent 2 Kole 192552 pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA CAG tdTomato Constitutive...

  15. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... 37354 pQC NLS mCherry IX Nucleus NLS mCherry Connie Cepko 37341 pQC NLS YFP IX Nucleus NLS YFP Connie...pCaggs-NLS-PAmCherry1-GSS-EGFP Nucleus NLS mGold Francois St-Pierre 14893 pEBFP2-Nuc Nucleus NLS EBFP2 ...Nuc Nucleus NLS mKalama1 Robert Campbell 73205 pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3 Nucleus NLS (from Mak16p...98875 3xnls-mNeonGreen Nucleus 3xNLS mNeonGreen Dorus Gadella 98817 3xnls-mTurquoise2 Nucleus 3xNLS mTurquoise2...mTurquoise2 Dorus Gadella 98816 3xnls-mScarlet-I Nucleus 3xNLS mScarlet-I Dorus Gadella 98876 4xmts-mNeonGreen...

  16. Retrograde AAV viral preps

    Type
    Collection
    ...Flp-dependent Control Deisseroth 99130 pAAV-mDlx-NLS-mRuby2 Dlx NLS-mRuby2 Control Gradinaru 83900 pAAV-mDlx-...Color-flipping switch. Expresses NLS-mCherry in the absence of Cre, and expresses NLS-EGFP in Cre-positive cells...Recombinases Janelia 51085 AAV-hSyn1-GCaMP6f-P2A-nls-dTomato Syn GCaMP6f and physically separate nuclear...Calcium sensor Ting 51083 AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f and physically separate nuclear...Calcium sensor Fishell 51084 AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s and physically separate nuclear...

  18. Brain Initiative Collection

    Type
    Collection
    ...Gradinaru 104061-PHPeB CAG-NLS-GFP AAV expression of nuclear localized GFP with SV40 NLS Viviana Gradinaru 105448...of the tTA Viviana Gradinaru 99130-AAV1 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized... enhancer Viviana Gradinaru 99130-AAV2 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized... enhancer Viviana Gradinaru 99130-AAV8 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized... enhancer Viviana Gradinaru 99130-AAV9 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized...enhancer Viviana Gradinaru 99130-AAVrg pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized...enhancer Viviana Gradinaru 99130-PHPeB pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized...

  19. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...region of a reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) a RFP protein that binds to the 3’ UTR of... of the reporter mRNA via an MS2 coat protein (NLS-MS2-RFP) the reporter mRNA, which contains the tethering...are transported back to the nucleus (thanks to a NLS on these proteins). The RFP proteins, however, remain...wild-type Cas9 into n-terminal (Cas9(N)-2xNLS) and c-terminal (Cas9(C)-2xNLS) fragments facilitates target DNA...fused with FK 506 binding protein 12 (Cas9(C)-FKBP-2xNLS) and the n-terminal cas9 fragment with FKBP rapamycin...rapamycin induces Cas9(N)-FRB-NES and Cas9(C)-FKBP-2xNLS dimerization and net influx into the nucleus, where...using dCas9-VP64 activator fragments (dCas9(C)-FKBP-2xNLS-VP64 and dCas9(N)-FRB-NES). This system provides...
Showing: 1 - 20 of 45 results