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We narrowed to 314 results for: Cas9

Showing: 201 - 220 of 314 results

  1. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...species or PAM binding variant of Cas9. For instance, wild-type SpCas9 must be used with targets that are...increases off-target effect. Which species or variant of Cas9 ( S. pyogenes , S. aureus etc.) was this gRNA sequence...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  2. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...genomic deletions. Compared to deletions generated by Cas9, large deletions created using Cas3 have fewer off-target...underutilized compared to their Class 2 counterparts like Cas9. This is attributed in part to the difficulties ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  3. Important Considerations When Using AAVs

    Type
    Blog Post
    ...Currently, AAVs are being used to deliver CRISPR/Cas9 but have the potential to deliver other therapeutic...used with new exciting technologies (such as CRISPR/Cas9) as they allow for gene delivery in an efficient...Expression in Mammals A Match Made in Heaven: CRISPR/Cas9 and AAV Viral Vector Elements Additional Resources...

  5. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ... ~70kDa, with a molecular weight roughly half of Cas9 or Cas12a. This is in part due to the fact that ...CRISPR Plasmids and Resources page.  CDDR (CRISPR–Cas9-based Dual-fluorescent DSB Repair) is a double-strand...by Kivanc Birsoy. Find the library here A new SaCas9 ABE variant (microABEI744) has improved on-target...footprint compared to current N-terminal linked SaCas9 ABE variants. It's also one of the smallest AAV-deliverable...

  6. Hot Plasmids - October 2020

    Type
    Blog Post
    ... codon-optimized, engineered Streptococcus canis Cas9 nuclease that has a broad 5′-NNG-3′ PAM. Get the...Applications (CHyMErA) Knockout Libraries express hybrid Cas9-Cas12a gRNAs under a single U6 promoter. The paralog...

  8. Plan Your Experiment

    Type
    Guide
    ...). You will decide how to express Cas9, the delivery system for Cas9 and the guide RNA (gRNA), the design...non-homologous end joining (NHEJ) at the cut site Cas9 or Cas9 nickase High-fidelity Cas enzymes increase specificity...insertions, by copying from a DNA donor template Cas9 or Cas9 fusions with proteins that promote HDR Requires...site. Consider alternative Cas9 enzymes with different PAM sequences or Cas9 enzymes with flexible PAM .../or Cas9 expression or (2) a lack of efficient target cleavage in cells that do express both Cas9 and ...edits without double-strand breaks Base editor (dCas9 or Cas9 nickase fusions with different base editing ...We will focus on making single edits using CRISPR/Cas9 in mammalian cells as an example, but many of these...

  9. CRISPR Cheat Sheet

    Type
    Blog Post
    ...the gRNAs to cut particular DNA or RNA sequences. Cas9 is a very commonly used CRISPR nuclease. Note:...versions of CRISPR nucleases that can’t cut DNA (dCas9, for example) that are used to increase or decrease...

  10. Call for Guest Bloggers

    Type
    Blog Post
    ...editing. Post: Generating Mouse Models Using CRISPR/Cas9   Erik L. Snapp, Associate Professor at Albert Einstein...

  11. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...Resources and references References Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...Brigham MD, Sanjana NE, Zhang F. Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

  12. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...from single or multiplex CRISPR-Cas9 double-strand DNA breaks using SpCas9. More features will be added ...Currently, ICE is configured for Cas9 fromStreptococcus pyogenes (SpCas9). ICE Score - The editing efficiency...Addgene Blog Browse our CRISPR featured topic page xCas9: Engineering a CRISPR Variant with Pam Flexibility...

  13. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...2023). This method uses a prime editor (typically a Cas9 H840A nickase fused to a reverse transcriptase) ...Steensel, B. (2021). Impact of chromatin context on Cas9-induced DNA double-strand break repair pathway balance...Yoon, S., & Kim, H. H. (2019). SpCas9 activity prediction by DeepSpCas9, a deep learning–based model with... and PE5 prime editing strategies) based on a DeepSpCas9 score ranking (Kim et al., 2019). PRIDICT saves...

  15. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...1st time CRISPR users Learn how to Choose the Best Cas9 Variant for Your Next Experiment Learn More About... Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic Regions of Interest...co-expressed, the gRNA targets the dCas9 to the loci of interest and dCas9 is biotinylated by BirA. From here...To increase the resolution, the Xu lab created a dCas9-based CAPTURE (CRISPR Affinity Purification in situ... cell line that co-expresses three components: dCas9 with an added biotin acceptor site BirA, a biotin...be pulled down via streptavidin IP. Biotinylated dCas9 goes along for the ride throughout all of these ... CAPTURE cell lines will have varying levels of dCas9 and gRNA expression. Additionally, CAPTURE 3C-based...

  17. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...unique sgRNAs into a multi-TU targeting construct (Cas9, sgRNA, reporter, etc.). This allows for the multiplexing...repurposed for CRISPR applications (gene insert = Cas9 + gRNA), Addgene distributes specialized CRISPR ...High-Efficiency Genome Editing in Plants Mediated by a Cas9 Gene Containing Multiple Introns.” Plant Communications...

  18. Modular Cloning Guide

    Type
    Guide
    ...CRISPR/Cas9 vectors expressing multiple gRNAs (up to seven) with a Cas9 nuclease, nickase, dCas9, or dCas9...mechanisms. zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9 Genome Engineering...assembly of CRISPR/Cas9 constructs for use in plants. The constructs are made with zCas9i, a high efficiency...biology and multigene engineering, including CRISPR/Cas9 components. MoChlo: Modular Cloning Chloroplast ...CRISPR Arnold Driessen 96 plasmids (including CRISPR/Cas9 components) for expression or gene editing in filamentous...proximity labeling experiments. Multiplex CRISPR/Cas9 Assembly Systems Genome Engineering, CRISPR, Mammalian...Expression Brigitte Gasser Plasmids to perform CRISPR/Cas9 mediated genome editing in the yeast P. pastoris...

  19. Editor's Choice, October 2016

    Type
    Blog Post
    ...Community Engineering the Plant Genome Using CRISPR/Cas9 Healthcare Consulting: A Door to the Business of...

  20. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ... hampered by the fact that even small Cas9 homologs like SaCas9 and their requisite gRNAs take up essentially...: PMC3277219. 6. Ohmori, Tsukasa, et al. "CRISPR/Cas9-mediated genome editing via postnatal administration...et al 2017 use a dual vector system composed of SaCas9 and a gRNA in one AAV and a repair template in ...
Showing: 201 - 220 of 314 results