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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 314 results for: Cas9

Showing: 241 - 260 of 314 results

  1. Neurodegeneration Research Collection

    Type
    Collection
    ...Target neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent rodent...species. Boender et al. Sci Adv. 2023 Jun 2. Use Cas9 in astrocytes. Endo et al Science. 2022 Nov 4. Base...

  2. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Addgene. CRISPR/Cas9 Delivery rAAV vectors are a popular choice for the delivery of CRISPR/Cas9 elements, making... genome editing. Packaging the Cas9 from Streptococcus pyogenes (SpCas9) and a gRNA together into an rAAV...this obstacle, has been to use smaller Cas9 orthologs, such as SaCas9 from Staphylococcus aureus . The Feng...too large to fit within the rAAV capsid, such as Cas9-derived plasmids and base/prime editors, is to create...Feng Zhang lab has developed plasmids containing SaCas9 optimized for their use in rAAV vectors . Read ...

  6. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...) model consists of a catalytically dead Cas9 protein (dCas9) that lacks endonuclease activity coupled.... Using dCas9 as an on/off switch The multiple scRNAs all individually utilize the same dCas9 protein....modulate dCas9 expression, multiple scRNA transcriptional programs can be regulated with dCas9 as a master...an orthogonal dCas9 protein that will still recognize guide RNA but with a different dCas9 binding module...specific proteins via aptamer domains. Combined with dCas9, this new scaffold RNA (scRNA) will bind to a PAM-adjacent...layered with distinct programs controlled by each dCas9 regulatory switch. By no means limited to the field...applied to effectively change metabolic flux. The dCas9 protein and orthogonal variants can be used as regulatory...

  7. Guide to Using Pooled Libraries

    Type
    Guide
    ...information on genome-wide screening with CRISPR/Cas9 . Browse our Viral Vector resources . Check out ...popular libraries! Genome-wide Screening Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale,...

  8. Adenovirus Guide

    Type
    Guide
    ... or when needing to package larger cargo. CRISPR-Cas9 Delivery As compared to other viral vectors, rAdV... vectors. This is the case of some commonly used Cas9 homologs, especially when having to express them...vectors. This capacity is large enough to carry the Cas9 gene and a gRNA expression cassette in one single...and Troubleshooting Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells More...

  9. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas Teusink Lab Materials...

  13. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...depositors have developed heat shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans...

  14. How Addgene Creates Content

    Type
    Blog Post
    ...changes and require updating or retiring. CRISPR/Cas9, for example, has gone through rapid change in the...

  15. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...primer for beginners Adenoviral delivery of CRISPR/Cas9 Tips for a first time AAV user Resources on Addgene.org...

  20. Distribution to Industry

    Type
    Collection
    ...Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome editing of human TP53...
Showing: 241 - 260 of 314 results