We narrowed to 439 results for: %s

... then the MoClo YTK Kit specifically designed for S. cerevisiae is at your service (Lee et al., 2015)....al., 2017). In addition to additional elements for S. cerevisiae chromosomal integration and gene expression...//doi.org/10.1021/acssynbio.9b00322. Kim, Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling...

...precise deletion products compared to monomeric Cas9’s. Read the paper in Nature Communications Find ...

...targets. Rajalingam K, Schreck R, Rapp UR, Albert S. Biochim Biophys Acta. 2007 Aug;1773(8):1177-95. PubMed...

...nucleotides of DNA can serve as the target for Cas9’s single guide RNA (sgRNA), provided it meets two conditions...system uses a “nicking” Cas9, in which one of Cas9’s two critical enzymatic residues has been converted...

...References and resources References Im K, Mareninov S, Diaz MFP, Yong WH (2018) An Introduction to Performing...fluorescence-microscopy/   Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez...

...Matho, K. S., Cetin, A., Woodard, K., Cepko, C., Guerin, K. I., Rego, M. A., Ersing, I., Bachle, S. M., Kamens...

...2412-2420. PubMed PMID: 25101481. Filonov, Grigory S., et al. "Broccoli: rapid selection of an RNA mimic...Central PMCID: PMC4923196. Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein purification and characterization...

... transposase. The donor plasmid contains the gene(s) of interest flanked by terminal repeat sequences,...They contain only a eukaryotic promoter and the cDNA(s) to be expressed and they don’t integrate or replicate...from this reference. 1. Malik, Nasir, and Mahendra S. Rao. "A Review of the Methods for Human iPSC Derivation...

...Ferreira DM, Krook A, Barres R, Zierath JR, Erhardt S, Lindskog M, Ruas JL. doi: 10.1016/j.cell.2014.07.051...

...unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence...

...iRFP713, % Oligomeric state Photo-stabilityc, t1/2, s pKa Brightness in HeLa cells vs. iRFP713, % d Ref...239 (2020). Oliinyk, O.S., Shemetov, A.A., Pletnev, S., Shcherbakova, D.M. & Verkhusha, V.V. Smallest near-infrared...

...involves making specific knock-out edits in a gene(s) of interest and has an average off-target frequency...CRISPR experiment? CRISPR 101: Cas9 The Other Cas(s) CRISPR 101: Validating Your Genome Edit         ...

...://doi.org/10.7554/elife.06100 Liu, L. S., Zhao, L. Y., Wang, S. H., & Jiang, J. P. (2016). Research proceedings...

...orthogonal nuclease-inactive Cas9's (dCas9) from S. pyogenes, N. meningitidis and S. thermophilus which have been...favorite cells. Ma et al., Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112...620nm) portions of the spectrum. PhD student Benjamin S. Padman recently developed a mitochondrially-targeted...context. Chatterjee A et al. Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. Plasmid tools for...

...variants, resulting in mScarlet3-S2 (M163H/M66Q; the "S" is for "stable") (Ding et al., 2026). mScarlet3-S2...025-02962-2 Xiong, H., Chang, Q., Ding, J., Wang, S., Zhang, W., Li, Y., Wu, Y., … Fu, Z. (2025). A highly...

...Hiraizumi, M., … Abudayyeh, O. O., & Gootenberg, J. S. (2025). Reprogramming site-specific retrotransposon...Sanchez, A. D., Udeshi, N. D., Svinkina, T., Carr, S. A., Feldman, J. L., Perrimon, N., & Ting, A. Y. (...

...like Antibodypedia or through your antibody provider(s) if they accept and share user data on the antibody...10.1016/j.nbt.2018.08.002  Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez...

...topics. Resources and references References Aydin S (2015) A short history, principles, and types of ELISA...10.1016/0003-2697(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender...

.... Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D, Young RA, Jaenisch R (2016) Editing...plasmids from this publication at Addgene. Stolzenburg S, Beltran AS, Swift-Scanlan T, Rivenbark AG, Rashwan...

...lately (1, 2), depends on knowledge of the structure(s) a given phage will recognize on a host cell. To ..., Callum J., Mohammadali Khan Mirzaei, and Anders S. Nilsson. "Adapting drug approval pathways for bacteriophage-based...