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Learn about the most common injection routes in mice, including their key differences and uses.

An in-depth look at fixing and permeabilizing samples in immunofluorescence experiments.

Learn about the different types of stem cells in this guide. In particular, we discuss the pros and cons of using embryonic stem cells and adult stem cells and using induced pluripotent stem cells (iPSCs) as a model for regenerative medicine.

Learn how rabies virus can be used to trace neuronal connections. AAV available at Addgene can help you with the process!

Learn about xCas9, an engineering Cas9 variant with broad PAM flexibility and high on-target specificity.

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.

Learn how to use the pPSU plasmids to generate affordable DNA ladders.

Ready-to-use AAV available from Addgene's viral service encoding tet-off transactivators and tools for affinity purification (TRAP).

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Addgene's antibody lab member Kate describes each step in recombinant antibody production process.

Ever wondered how Addgene makes our viral preps? We explain it all here!

Pooled libraries are a powerful tool, but they can difficult to prepare! Learn the tips and tricks for taking your pooled libraries from plasmids to screening.

Course based undergraduate research classes use lab time to dive into real-world experiments. This guest post introduces CURE concepts and application.

Bimolecular fluorescence complementation is a method that uses split fluorescent proteins tethered to two potential protein interaction partners.

REPAIR and RESCUE are two CRISPR tools for RNA editing based on Cas13b.

Sarting a journal club? Read our guide to the logistics of forming a journal club and find different formats to hold the journal club discussion.

Find tips and tricks for using digital droplet PCR to quantify your AAV titers.

Find plasmids more easily with the new search feature on Addgene's website! Now, you can set filters to narrow down your plasmid or viral service search.

Crossing the blood brain barrier is difficult for many AAVs. But, PHP.B has impressive CNS transduction in some mice. Learn more about how PHP.B and about its strain specific performance.

Find tips for transforming arabidopsis with your plasmid construct of interest.