We narrowed to 305 results for: Cas9

...amplification, new toehold switch designs, CRISPR/Cas9-mediated genotyping, as well as our electronic reader...

...primer for beginners Adenoviral delivery of CRISPR/Cas9 5 tips for troubleshooting viral transductions ...

...well plate for further analysis. To assess CRISPR/Cas9 knockout efficacy, we have used both Western blotting...

... the guide from binding to the genome or prevent Cas9 from cutting, both of which can introduce significant...

...Split-horseradish peroxidase (Alice Ting Lab 2016)  Split-Cas9 for inducible genome editing (Feng Zhang Lab 2015...

...   For CRISPR libraries, Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

... blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24 for simplifying plant genetic engineering...

...the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes prefer targets with a given...

...edits, such as long or highly structured templates. Cas9 variants PE6e–g often had even higher editing efficiencies...

..., N. E., & Zhang, F. (2017). Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

...Screening Read about Genome-Wide Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out ...

...resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of additional...

...cellular RNA to specific intracellular locations using Cas9 and track the localization of the complex. Broccoli...

...CRISPR-Cas9 genome editing may be the hot new way to manipulate gene expression, but other gene manipulation...

...sequential homology directed repairs (HDR) with CRISPR/Cas9. Donor A contains: 400bp homology arms to the site...

...endogenous tagged genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for endogenous...

... Addgene Blog Genome-wide Screening Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale,...

...synthesize a target metabolite, genetic tools like CRISPR-Cas9 are not only valuable but often essential. Find ...Plasmid Promoter Inducer Expression Species PI 44249 pdCas9-bacteria pTetO Anhydrotetracycline (aTc) Escherichia...

...tet-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP...Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9-VPR transcriptional activator...DHFR-EF1a-TagRFP-2A-tet3G Tet-inducible expression of KRAB-dCas9-DHFR for CRISPRi. See Plasmid #167937 for CRISPRa...

...Bassik MC (2016) Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes. Nat Biotechnol...