We narrowed to 314 results for: Cas9
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TypeCollection... Separate expression pRGEB31 - Deliver sgRNA and Cas9 into plants by Agrobacterium-mediated transformation...Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated... FP Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions...
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Overcoming the Challenges of Lentiviral Production
TypeBlog Post...single 72h harvest is best for larger genes such as Cas9. See figure 1 for an example of how changing transfection... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog Post...community has created hundreds of useful variants of Cas9. By providing access to the plasmids encoding antibodies... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post..., Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog Post...amplification, new toehold switch designs, CRISPR/Cas9-mediated genotyping, as well as our electronic reader... -
Pooled Library Amplifications
TypeBlog Post... For CRISPR libraries, Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog Post... the guide from binding to the genome or prevent Cas9 from cutting, both of which can introduce significant... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post...well plate for further analysis. To assess CRISPR/Cas9 knockout efficacy, we have used both Western blotting... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post...primer for beginners Adenoviral delivery of CRISPR/Cas9 5 tips for troubleshooting viral transductions ... -
CRISPR Pooled gRNA Libraries
TypeCollection...require the use of lentivirus to deliver the gRNA/Cas9 to target cells. Therefore, you must be equipped...86550 Inhibition Human Weissman 3rd 10 Varies CRISPR/Cas9-assisted Removal of Mitochondrial DNA (CARM) Library...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors... Sabatini and Lander 3rd 50 6,661 Garnett Lab MinLibCas9 Library 164896 Knockout Human Garnett 3rd 2 37,722... -
Hot Plasmids - November 2023
TypeBlog Post...edits, such as long or highly structured templates. Cas9 variants PE6e–g often had even higher editing efficiencies... -
Tips for arabidopsis transformation
TypeBlog Post... blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24 for simplifying plant genetic engineering... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes prefer targets with a given... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...Split-horseradish peroxidase (Alice Ting Lab 2016) Split-Cas9 for inducible genome editing (Feng Zhang Lab 2015... -
Lentiviral Vector Uses and Overview
TypeBlog Post...Screening Read about Genome-Wide Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out ... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog Post...cellular RNA to specific intracellular locations using Cas9 and track the localization of the complex. Broccoli... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post...CRISPR-Cas9 genome editing may be the hot new way to manipulate gene expression, but other gene manipulation... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...sequential homology directed repairs (HDR) with CRISPR/Cas9. Donor A contains: 400bp homology arms to the site... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post...endogenous tagged genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for endogenous... -
Cre-Lox and Other Site-Specific Recombinases
TypeCollection...vivo using a mouse line expressing Cre-dependent Cas9. Kits Kit Name Type PI Description dRMCE Plasmid...