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We narrowed to 449 results for: crispr

Showing: 261 - 280 of 449 results

  1. Plan Your Experiment

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    ... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan ...information on the wide range of CRISPR technologies, visit our CRISPR Guide . There are many different...framework to get you started using CRISPR in your research and setting up CRISPR experiments. We will focus on...systems utilized for CRISPR/Cas gene editing. This image summarizes ways that the CRISPR/Cas system can be...transcription elongation. CRISPR Activation : Target the transcription start site. CRISPRa requires dCas9 to ...Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning...Expression and Delivery Validate Genetic Edit References CRISPR ( C lustered R egularly I nterspaced S hort P alindromic...

  2. CRISPR Plasmids - gRNAs

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    ... CRISPR gRNAs CRISPR Plasmids: gRNAs Browse CRISPR Plasmids By Function Genome...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...PAM sequence. Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments...experiments use targeting sequences within exons, whereas CRISPR activation or repression experiments use targets...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity...containing gRNA sequences designed to be used with any CRISPR nuclease and function. Please see the publication...

  3. CRISPR Plasmids - dCas9-FokI

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A...FokI CRISPR: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...off-target effects of CRISPR. Browse, sort, or search the tables below for CRISPR-FokI plasmids. Plasmids...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR... CRISPR dCas9-FokI ...

  4. CRISPR Plasmids - Single-Strand Break (Nick)

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    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR/Cas... CRISPR Nick CRISPR Plasmids: Nick...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR...Nick Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR nickase plasmids. Plasmids are available for expression...

  5. CRISPR Plasmids - RNA Targeting

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type...CRISPR RNA Targeting CRISPR Plasmids: RNA Targeting Browse CRISPR Plasmids By Function Genome Editing Cut ...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR... Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. In ... of Cas enzymes? CasPEDIA is an encyclopedia of CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR RNA targeting plasmids. Plasmids are available ...

  6. CRISPR Plasmids for Genomic Visualization

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Using... CRISPR Visualize CRISPR Plasmids:...CRISPR/Cas plasmids for labeling and visualization of genomic loci. CRISPR...Plasmids: Visualize Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...fluorescent proteins to visualize targeted genomic loci. CRISPR imaging has numerous advantages over other imaging...like fluorescence in situ hybridization (FISH), CRISPR imaging offers a unique method for detecting the...chromatin dynamics in living cells. The fluorescent CRISPR system has been used for dynamic tracking of repetitive...

  7. CRISPR Plasmids - Zebrafish

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Zebrafish CRISPR Plasmids: Zebrafish...following CRISPR plasmids have been designed for use in zebrafish. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in zebrafish. CRISPR...Zebrafish Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...selectable marker or cloning method. When using CRISPR, you will need to express both a Cas protein and...

  8. CRISPR Plasmids - Repress Gene Expression

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically... CRISPR Interfere CRISPR Plasmids: Interfere...CRISPR plasmids for transcriptional repression of target genes. CRISPR...Interfere Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR plasmids for transcriptional inhibition. Plasmids...

  9. Plant Plasmids and Resources

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    ... Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology...like... Plant CRISPR resources Fluorescent Protein Guide Addgene Blog: Plant Biology CRISPR Cloning Transcription...Rice - Yinong Yang. The MoClo CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors,...technology or the Plant CRISPR Resources for a full selection of plasmids expressing different functional variants... gRNA backbones. A few examples of useful plant CRISPR resources include: A Multipurpose Toolkit enables...truncatula , wheat, and barley - Daniel Voytas. A CRISPR/Cas9 Toolkit for use in a variety of plant species...series of vectors and DNA modules for assembly of CRISPR/Cas9 constructs - Sylvestre Marillonnet and Johannes...

  10. CRISPR Plasmids - Double-Strand Break (Cut)

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully... CRISPR Cut CRISPR Plasmids: Cut Browse CRISPR...CRISPR plasmids that create double strand breaks in DNA. CRISPR... Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR plasmids designed to introduce a DSB. Plasmids ...

  11. Microbiology Resources

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    ...Microbiology General CRISPR Resources Read our CRISPR Guide for a general introduction to CRISPR technology. Check...software. Addgene yeast CRISPR resources Bacterial CRISPR Resources The CRISPR technology was developed...synthetic biology. CRISPR Resources : Find essential background information on using CRISPR in your lab, specific... Check out our CRISPR reference page for CRISPR resources, such as depositor protocols and gRNA design...Find CRISPR tools for use in genome editing of bacteria. CRISPathBrick Multiplex Plasmid : CRISPR expression... specific CRISPR tools for genome editing in bacteria, and more. Synthetic Biology : Browse our deposited...editing is not always straightforward. Read our CRISPR Methods for Bacterial Genome Engineering blog post...

  12. CRISPR Plasmids - Activate Gene Expression

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically...Activate CRISPR Plasmids: Activate Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR... CRISPR Activate...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR plasmids for transcriptional activation. Plasmids...

  13. CRISPR Plasmids - Xenopus

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Xenopus CRISPR Plasmids: Xenopus Browse...CRISPR plasmids that have been designed for use in Xenopus. CRISPR... Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation... The following CRISPR plasmids have been designed for use in Xenopus. Cut Fully functional Cas9 enzymes...selectable marker or cloning method. When using CRISPR, you will need to express both a Cas protein and...

  14. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    ...increasing the size of the AAV donor template for CRISPR-mediated homology directed repairs (HDR). Split...sequential homology directed repairs (HDR) with CRISPR/Cas9. Donor A contains: 400bp homology arms to ...single step and serve as donors for two consecutive CRISPR-mediated HDR events which results in the integration....org/10.1172/jci34316 Bak RO, Porteus MH (2017) CRISPR-Mediated Integration of Large Gene Cassettes Using...

  15. CRISPR Plasmids - Base Edit

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two...CRISPR plasmids for precisely editing DNA bases without inducing a DNA break. CRISPR... CRISPR Base Edit CRISPR...CRISPR Plasmids: Base Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...AID-mediated mutagenesis (TAM) from the Chang lab and CRISPR-X from the Bassik lab , in which Cas9 is fused ...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR base editing plasmids. Plasmids are available for...

  16. CRISPR Plasmids - C. elegans

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR C. elegans CRISPR Plasmids: C. ...following CRISPR plasmids have been designed for use in C. elegans. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in C. elegans. CRISPR.... elegans Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...selectable marker or cloning method. When using CRISPR, you will need to express both a Cas protein and...

  17. CRISPR Plasmids - Drosophila

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...CRISPR plasmids that have been designed for use in Drosophila and other insects. CRISPR... CRISPR Drosophila CRISPR...CRISPR Plasmids: Drosophila Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime... The following CRISPR plasmids have been designed for use in Drosophila and other insects. Cut Fully functional... functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based...Plasmid Gene/Insert Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...

  18. Zebrafish Plasmid Collection

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    ...Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology...examples of useful zebrafish CRISPR resources can be found below: UFlip CRISPR targeted integration vectors...for Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression...Joung Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector...modified CRISPR-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference...for the laboratory use of zebrafish. CRISPRz - A database of CRISPR/Cas9 target sequences that have been...morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make the zebrafish a stalwart...

  19. CRISPR Plasmids - Mammalian Expression

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base...CRISPR plasmids that have been designed for use in mammalian systems. CRISPR... CRISPR Mammalian Expression CRISPR...CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids have been designed for use in mammalian...mammalian expression systems. Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (...Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit...->G (or T->C on the opposite strand) mutations. CRISPR-X, a base-editing system from the Bassik lab, uses...

  20. Genomic Deletions in Mammalian Cell Lines

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    ... CRISPR References & Information Genomic Deletions in Mammalian Cell Lines CRISPR: Protocol...Mammalian Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud...regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system may be re-purposed.... Perform maxi-prep for each CRISPR/Cas9 construct. Transfecting CRISPRs into Cells of Interest NOTE: ...using CRISPR/Cas9 CRISPR...Orkin's and Daniel Bauer's labs details the use of CRISPR/Cas9 to create genomic deletions in mammalian cell...of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9. Bauer DE, Canver MC, Orkin SH. J. Vis. Exp...
Showing: 261 - 280 of 449 results