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...non-physiological RNA-protein interactions due to sample processing. Require prior knowledge that the protein...control). BioID2 had no detectable signal at the same time point. E. coli’s BirA* was tested at its optimum...

...isolated which target and cleave RNA through the same mechanism as traditional spCas9. If you are looking...Cas13 and has been proposed to be useful for the same applications (RNA knockdown, RNA editing, etc.)....

...interpret the results to see if it leads you to the same conclusion. Personally, I like papers that can end...figures and write down your own results; are they the same as the description the authors give? Ask yourself...

...& Walton et al). Additionally, SpG Cas9 with the same PAM preference of NG was isolated by the Kleinstiver...several bps away from a desired site does not have the same devastating impact on efficiency as 10-15 bps away...

... viruses are more dangerous than others, and the same holds true for types of viruses generated in the...personal protection are key to lab safety and the same principles apply to viral handling. Gloves are always...

...reducing editing from ~30% in control samples to 0-10% in Acr samples. Acr proteins can also be used to prevent...

...particles = number of particles per mL of sample. The purity of the sample can be estimated by looking at the...

...necessary and sufficient for retrograde transmission (Etessami et al., 2000, Wickersham et al., 2007). Thus, ...Mar 19;110(12):E1152-61 Pubmed PMID 23487772. 3. Etessami R, Conzelmann KK, Fadai-Ghotbi B, Natelson B, ...

...Students can produce DNA templates via PCR off of the same plasmid and use these to create riboprobes for in...WISH protocol involves preparation of the tissue sample, several washes, and temperature changes to visualize...

...contact Addgene. We may require that you send your samples to Addgene pre-transformed in this strain. ...with instructions on how to prepare and ship the samples to Addgene and contact your technology transfer...

...your poster. While a poster generally contains the same sections as a primary research article, it’s important...four column style, and in all cases the idea is the same; the reader will navigate the poster from column...

...between the sites. If a DNA sequence is floxed in the same orientation, the SSR will excise the DNA fragment...reporter with one loxP site and one lox511 site in the same orientation, and placing the additional loxP and...

...answers uninteresting, the panel members all say the same thing and one panel member dominates the discussion... for an intimate event, put the panelists on the same level as the audience. In my experience the best...

... synthetic biology lab Keira and I worked in the same lab for four years. It was a cutting-edge synthetic... on the detector plants, we expected to see that same anger. Instead, social media comments were more ...

...vivo. The general process of these screens is the same: Deliver CRISPR library to cells of interest. Select...Low, S. H., François, A., Suárez-Amarán, L., & Samulski, R. J. (2022). AAV vectors: The Rubik’s cube of...

..., so the amount of cloning required is about the same between insect and mammalian systems. Baculovirus...P1 virus, so that if you ever need to remake the same virus, you can skip the first round of production...

...known titer on every qPCR run. Note that the rAAV sample has to be digested with DNase before undergoing...Grimm, D., Kern, A., Pawlita, M., Ferrari, F., Samulski, R., & Kleinschmidt, J. (1999). Titration of AAV...

... this purpose.Learn about the effects of CO2 on sample pH Cell morphology: Shape and size matter You should...contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers Keep an eye on the...

...mammalian cells, finding xCas9 3.7 displayed about the same editing rate as SpCas9 at an NGG PAM. When they ...Adenine base editor xCas9 3.7-ABE also follows the same pattern, with improved editing at an NGG PAM (69...

...phenotype since additional genes that can do the same function are less likely to exist). If there’s an...to get males to recombine two mutations onto the same chromosome. You should use females for that. Now...