We narrowed to 3,091 results for: sam

1. pAAV-EF1a-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

   Plasmid

   #220612

   Purpose

   Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   FLAG-KORD-P2A-ArgiNLS-AausFP1
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG; ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDG1-PHP.eB

   Plasmid

   #220799

   Purpose

   AAV helper plasmid expressing E4, E2A, VA, AAV2 rep and PHP.eB cap genes

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   E4, E2A, VA, AAV2 rep and PHP.eB cap genes
   

   Use

   AAV; Aav helper vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. nLuc-PTHR1

   Plasmid

   #162577

   Purpose

   Expresses N-terminally NanoLuc-Tagged PTHR1 in mammalian cells

   Depositor

   Sam Gellman

   Article

   Yu et al ACS Chem Biol. 2022 Nov 18;17(11):3148-3

   Insert

   Parathyroid Hormone Receptor 1 (PTH1R Human)
   

   Use

   Tags

   Nanoluc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. nLuc-GLP-1R-His-FLAG

   Plasmid

   #124831

   Purpose

   Expresses N-terminally NanoLuc-Tagged GLP-1R in mammalian cells

   Depositor

   Sam Gellman

   Article

   Cary et al Nat Chem Biol. 2022 Mar;18(3):256-263. d

   Insert

   GLP1R (GLP1R Synthetic, Human)
   

   Use

   Luciferase

   Tags

   FLAG, His6, and NanoLuc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-CAG-FLEX-ArgiNLS-AausFP1

   Plasmid

   #220602

   Purpose

   Cre-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   AausFP1
   

   Use

   AAV and Cre/Lox

   Tags

   ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pL0-BvDODAα1

   Plasmid

   #162528

   Purpose

   Beta vulgaris 4,5-DOPA dioxygenase alpha 1 coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.

   Depositor

   Sam Brockington

   Article

   Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

   Insert

   DODAα1
   

   Use

   Puc19-derived

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-EF1a-ArgiNLS-mGreenLantern

   Plasmid

   #220596

   Purpose

   Constitutive expression of a single-cell discriminating version of mGreenLantern fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   mGreenLantern
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq)

   Plasmid

   #220611

   Purpose

   Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   FLAG-KORD-P2A-3x HA-hM3D(Gq)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG; ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO-shSmad4

   Plasmid

   #37046

   Depositor

   Sam Thiagalingam

   Article

   Papageorgis et al Cancer Res. 2010 Feb 1;70(3):968-78. Epu

   Insert

   Smad4 (SMAD4 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. nLuc-ΔECD-PTHR1

    Plasmid

    #190011

    Purpose

    Expresses the Nanoluciferase fused to the human parathyroid hormone receptor type 1 that is missing the extracellular domain

    Depositor

    Sam Gellman

    Article

    Yu et al ACS Chem Biol. 2022 Nov 18;17(11):3148-3

    Insert

    nLuc-ΔECD-PTHR1 (PTH1R Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pL0-MtPT4Pro

    Plasmid

    #159414

    Purpose

    Medicago truncatula PT4 promoter sequence (836 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Insert

    MtPT4 Promoter
    

    Use

    Puc19-derived

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pL0-MtBCP1Pro

    Plasmid

    #159415

    Purpose

    Medicago truncatula BCP1 promoter sequence (1108 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Insert

    MtBCP1 Promoter
    

    Use

    Puc19-derived

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBabe-puro-Smad4-Flag

    Plasmid

    #37041

    Depositor

    Sam Thiagalingam

    Article

    Papageorgis et al Cancer Res. 2011 Feb 1;71(3):998-1008. E

    Insert

    Smad4 (SMAD4 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

14. NbPT5b-p3

    Plasmid

    #160001

    Purpose

    Multigene construct containing the betalain pigment biosynthetic genes under the control of the NbPT5b promoter for visualisation of arbuscular mycorrhizal colonisation in Nicotiana benthamiana roots.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Inserts

    Bar (reverse orientation)
    DODAα1
    CYP76AD1
    cDOPA5GT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Agrobacterium tumefaciens Nopaline synthase Promo…

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NbBCP1b-p3

    Plasmid

    #160002

    Purpose

    Multigene construct containing the betalain pigment biosynthetic genes under the control of the NbBCP1b promoter for visualisation of arbuscular mycorrhizal colonisation in Nicotiana benthamiana roots

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Inserts

    Bar (reverse orientation)
    DODAα1
    CYP76AD1
    cDOPA5GT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Agrobacterium tumefaciens Nopaline synthase Promo…

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. saposin B^W

    Plasmid

    #161759

    Purpose

    Saposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophan

    Depositor

    Sam Gellman

    Article

    Kurgan et al Biochemistry. 2021 Apr 13;60(14):1108-11

    Insert

    saposin B R38_W
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    changed Arginine 38 to Tryptophan

    Promoter

    T7

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-hSyn-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

    Plasmid

    #220608

    Purpose

    Neuron-specific, Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
    

    Use

    AAV and Cre/Lox

    Tags

    3x HA; ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-hSyn-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

    Plasmid

    #220610

    Purpose

    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    FLAG-KORD-P2A-ArgiNLS-AausFP1
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG; ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO-shSmad2

    Plasmid

    #37051

    Depositor

    Sam Thiagalingam

    Article

    Papageorgis et al Cancer Res. 2010 Feb 1;70(3):968-78. Epu

    Insert

    Smad2 (SMAD2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

20. MtPT4-p3

    Plasmid

    #160003

    Purpose

    Multigene construct containing the betalain pigment biosynthetic genes under the control of the MtPT4 promoter for visualisation of arbuscular mycorrhizal colonisation in Medicago truncatula roots.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Inserts

    DsRed (reverse orientation)
    DODAα1
    CYP76AD1
    cDOPA5GT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Arabidopsis thaliana Ubiquitin 10 Promoter (AtUb1…

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge