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PB-UniSAM
(Plasmid #99866)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 99866 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Piggy Bac
  • Backbone size w/o insert (bp) 3961
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology ; dCAS9-SAM activation
  • Selectable markers
    mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    UniSAM-mCherry + U6-gRNA2.0
  • Alt name
    Cas9VP64-MS2p65HSF1-mCherry + gRNA(2xMS2Loops)
  • Insert Size (bp)
    8388
  • Mutation
    BbsI sites in CDS were ablated by consensus mutation codon optimised for H.sapiens
  • Promoter EF1a

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer EXT_PB_RV--GGCACATATCAATATTATGCTCTCG
  • 3′ sequencing primer EXT_PB_FW--GCAAAGCGATGACGAGCTTG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-UniSAM was a gift from Lesley Forrester (Addgene plasmid # 99866 ; http://n2t.net/addgene:99866 ; RRID:Addgene_99866)
  • For your References section:

    An all-in-one UniSam vector system for efficient gene activation. Fidanza Antonella, Martha Lopez-Yrigoyen, Nicola Romanò, Rhiannon Jones, A. Helen Taylor & Lesley M. Forrester. Scientific Reports 10.1038/s41598-017-06468-6