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Pooled extracellular receptor-ligand interaction screening using CRISPR activation.
Chong ZS, Ohnishi S, Yusa K, Wright GJ
Genome Biol. 2018 Nov 26;19(1):205. doi: 10.1186/s13059-018-1581-3.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
112919pKLV2-EF1adCas9VP64T2ABsd-WLentiviral vector expressing Lentiviral vector expressing Lentiviral vector expressing dCas9VP64 under the control of the long human EF1a promoter
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112920pKLV2-EF1aMS2p65HSF1hyg-WLentiviral vector expressing MS2p65HSF1 under the control of the long human EF1a promoter
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112921pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopAGateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker
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112922pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpAGateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker
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112923pPB-R1R2-EM7neoPheSpiggyBac Transposon vector with a Gateway destination cassette (neoPheS selection)
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112924pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpApiggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker
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112925pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-WLentiviral vector for SAM-CRISPRa gRNA expression with puroBFP
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112926pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-WCRISPR-a reporter assay lentiviral vector (empty gRNA control)
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112927pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-WCRISPR-a reporter assay lentiviral vector (gTetO)
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