|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||112925||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepKLV2 lentiviral vector
- Backbone size w/o insert (bp) 6330
- Total vector size (bp) 8698
Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert namehU6 promoter, gRNA-SAM expression cassette
SpeciesSynthetic; S. pyogenes
- Cloning method Gibson Cloning
- 5′ sequencing primer AGATAATTAGAATTAATTTGACTG (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W was a gift from Kosuke Yusa (Addgene plasmid # 112925 ; http://n2t.net/addgene:112925 ; RRID:Addgene_112925)