1,187 results

Introduction to immunofluorescence and how you can use it to illuminate your research.

Learn about the sources of spontaneous recombination and optimizing Cre-recombinase dependent AAV vector expression in vivo.

An antibody’s isotype is determined by its heavy-chain constant region and influences what role the antibody plays in an immune response.

Since several sets of near-infrared fluorescent proteins (NIR FPs) and biosensors have been created recently, we've added a quick guide to help you choose the best probe for your research needs.

Recent highlights from Addgene's collection include a system to examine biosynthetic pathways, and a new tool for enriching ABE-targeted cells.

Having trouble with viral vectors? Here are several tips to help prevent recombination in viral vectors or other plasmids with repeating elements.

Announcing Addgene's new Intro to the Lab Bench, a collection of protocols, videos, and quizzes to help guide those new to working in the lab.

ELISA are used to quantitatively detect protein concentrations in experimental samples. Learn about different types of ELISA and when to choose each method.

Get a preview of new science-themed GIFs from Addgene and learn how to find them on Twitter

The basics of PCR covering end point PCR, qPCR, ddPCR, and multiplex PCR and how each method is used.

See the winners for the 2021 Michael Davidson and Roger Tsien Commemorative Conference Award.

Meet Addgene's Newest Board Member Bart Newland.

Recent highlights from Addgene's collection include CRISPR-based editing in organoids and Biosensors for detecting strigolactones.

Get an overview of flow cytometry including how it works, what you can use flow cytometry for, and tips and tricks for a successful flow cytometry run.

Polyclonal antibodies are a common research reagent - learn how they’re produced and the advantages and disadvantages of using them in your next experiment.

Find plasmid tools for COVID-19 research expressing viral open reading frames, host factors, and tools for detecting SARS-CoV-2 and generating pseudoviruses.

An introduction to transposons including Sleeping Beauty, piggyBac, and Tol2 and how they can be used in transposon mutagenesis screens, gene transfer, and creating transgenic animals.

Get the basics on monoclonal antibodies including how monoclonal antibodies are made and why use monoclonal antibodies for your experiment.

Plasmids from Addgene encoding SARS-CoV-2 spike protein variants can be used to study antibody neutralization escape.

Use blue light to control gene expression from PBAD promoters. It's more spatially and temporally precise compared to AraC based expression vectors.