1,187 results

Should you codon optimize your gene or not? Here, we discuss several reasons for and against codon optimization.

The origin of replication is the DNA sequence which allows initiation of replication within a plasmid by recruiting transcriptional machinery proteins, enabling a plasmid to reproduce itself.

New channelrhodropsins, called ChRgers, avoid intracranial injections and fiber-optic cable implantation for in vivo optogenetics experiments.

Get the basics on RNA interference (RNAi), how it compares with CRISPR, and tools for RNAi studies in plants.

Find six out-of-the-box ideas for professional development in this virtual world.

Find the latest plasmids and viral vectors to available at Addgene in the October 2020 hot plasmids blog post!

Mycoplasma contamination? Find out where it comes from, how to detect it, prevent it, and remove it from your tissue culture experiments.

Choosing a CRISPR activator? This article features four of the most popular CRISPR activators (dCas9-VP64, SAM, SunTag, and VPR) and some newer activators like dCas9-CBP and SPH.

Model organisms have genetic and developmental characteristics that make them easy to study. Examples include E. coli, rat, frog, axolotl, and Arabidopsis.

Designing a CRISPR experiment in bacteira? Check out these tools for genome engineering, CRISPRa, CRISPRi, base editing, and microbiome engineering.

Practical advice for using CRISPRs: designing your gRNA and introducing it into cells.

Thinking about a career in science policy? In this article, we cover the who, what, when, where, why, and how of science policy careers.

The COVID-19 pandemic makes open science more important than ever. Read about how Addgene is weathering COVID-19 with support from the scientific community.

This post highlights ways to overcome the AAV size limitation to deliver CRISPR editing tools to cells.

Here, we cover four different plasmid screening strategies including blue-white screening, colony PCR, restriction digest analysis, and Sanger sequencing.

Learn about different strategies used to generate plasmids for multiplex gRNA expression and how to use them in various organisms.

Addgene supports rare disease research with the 2020 BeHEARD Awards. Read about the winners and their research in this blog post.

The tools of optogenetics can be combined with CRISPR to generate inducible genome edits and gene regulation systems.

Overview of split vector approaches for fitting genes >4.7 kB into AAV including overlapping, transplicing, hybrid, and sequential homologous recombination.

Bimolecular fluorescence complementation is a method that uses split fluorescent proteins tethered to two potential protein interaction partners.