1,187 results

Protospacer Adjacent Motifs (PAM) serve as a binding signal for Cas proteins. Here we highlight the many Cas proteins and their PAM sequence variations.

Learn about new plasmids and viral preps available at Addgene including a yeast-based tool for protein evolution, fluorescent tools, nanobody purification, new CRISPR tools, and calcium and norepinephrine biosensors.

Addgene marketing intern, Brandon Solis, shares his experience working virtually in a COVID-19 world and his Meet an Addgenie Instagram project.

Learn about the basic elements that make up the AAV transfer plasmid, including the ITRs, transgene, promoter, and other cis-regulatory elements.

Repositories provide fast access to wet lab research materials during COVID-19 such as cell lines, plasmids, and mouse strains.

REPAIR and RESCUE are two CRISPR tools for RNA editing based on Cas13b.

Marc Zimmer's book, The State of Science, examines broad issues in science including racism and sexism, peer-review, funding, CRISPR, and the politicization of science.

Use anti-CRISPR proteins to turn off genome editing and prevent off-target effects. In this article, read about how it works and its applications

Find details on five methods to quantify DNA: UV absorbance, fluorescence dyes, agarose gel electrophoresis, capillary electrophoresis, and diphenylamine.

David Liu's lab analyzed how COVID-19 affected their plasmid distribution.

Find seven tips for scientists when preparing for an industry interview, including advice for remote interviewing.

Use these CRISPR base editing reporters to track base editing events in real-time and enrich for base edited cells.

Learn all about CAPTURE - a new CRISPR-based technique to study regulatory elements in their native chromatin contexts.

Find tips for getting the best virtual conference experience from attending talks, poster sessions, networking, and taking advantage of the virtual format.

Our new Instagram series, Meet an Addgenie, features stories form Addgenies about their career decisions, paths, and more.

Epigenetic modifications are an additional layer of control over gene expression. Targeted epigenetic modification is possible using CRISPR tools from Addgene!

Addgene expands plasmid distribution to industry scientists during COVID-19 and beyond. Learn more about the collection in this blog post.

Use plasmids to develop synthetic nanobodies in a few weeks with a three step selection process.

Using plasmids for transformation, cloning, or transfection? Different uses mean you'll need different purities of DNA for your applications.

So you want to control neuronal activity, but should you choose chemogenetics or optogenetics? Read this article to see how these methods compare.