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We narrowed to 452 results for: crispr

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Showing: 281 - 300 of 452 results

  1. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...Addgene Blog Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User) The Challenges of Cell Culture...; more recently we have also been using Addgene CRISPR vectors to generate cell lines with knockouts of...the 48-well plate for further analysis. To assess CRISPR/Cas9 knockout efficacy, we have used both Western...

  2. Lentiviral Prep Service

    Type
    Collection
    ...Packaging Service page . Pooled CRISPR Libraries for Screening Use pooled CRISPR libraries to screen for genes...Discontinued) Human CRISPR inhibition library Dolcetto in XPR_050 (VP64) Set A Human CRISPR inhibition library...Discontinued) Human CRISPR inhibition library Dolcetto in XPR_050 (VP64) Set B Human CRISPR inhibition library...plasmids . For more information on CRISPR plasmids and function, see our CRISPR plasmids resource page . Cas9...Service Lentivirus Browse In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled Barcoding Libraries Control...

  4. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...zinc-finger nucleases, TALENs, and more recently CRISPR/Cas9. Another popular targeted gene deactivation...developed, relying on established reagents such as CRISPR/Cas9, Tol2, and the high-quality sequenced zebrafish...systems, and their resistance (so far) to facile CRISPR/Cas9-mediated insertion of foreign DNA. Despite... Resources on Addgene.org Browse our zebrafish CRISPR plasmid collection Read our molecular biology reference...

  5. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ...plasmids covered in this segment Hot Plasmid 1: CRISPR X from Michael Bassik's Lab - A new tool for making...localized point mutations. Listen to Our Segment on CRISPR X Hot Plasmid 2: Magneto 2.0 form Ali Guler's Lab...

  6. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...development in immunotherapy is the combination of the CRISPR/Cas9 methodology with T cell receptor engineering...contrast to using randomly integrating viral vectors, CRISPR/Cas9 genome editing allows targeted engineering... et al. "Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection." Nature 543.7643...

  7. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas amassed nearly 2,400 citations in its first...much, you can see the 2013 ‘bump’ related to the CRISPR/Cas papers and Addgene deposits, but papers published... Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (2013): 819-823....

  8. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...technique is also highly relevant in this age of CRISPR; site-directed mutagenesis generally applies to...DNA through homology-directed repair (HDR) of a CRISPR/Cas9 induced double-stranded break. This site-directed...a similar approach can be used to identify Cas9/CRISPR-induced genome modifications. Occasionally, multimerization...More about Plasmids in Our Plasmids 101 Series Use CRISPR to Generate Specific Genomic Mutations Resources...

  9. Plasmid Collections

    Type
    Collection
    ...plasmids, kits, and pooled libraries. Find tools for CRISPR, fluorescent proteins, optogenetics, biosensors... Genome Engineering Genome Engineering Overview CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent...pooled libraries. Pooled Library Types Barcoding CRISPR Screening Surface Display cDNA Depositor Collections...view all collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic...

  10. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    ...Architect Pam McA' Nulty (check out our blog post on CRISPR software to see the importance of programming for...Scientist Joel McDade’s presentation on the emerging CRISPR genome editing technology was a rousing success...

  11. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ... infiltration of CRISPR-Cas tools into neuroscience experiments. Speaking of CRISPRs, we were excited...

  12. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...Resources on the Addgene Blog Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn ...Addgene.org Check out Our Microbiology Resource Page CRISPR plasmids designed for use in bacteria Deposit Your...

  13. Bacterial Expression Systems

    Type
    Collection
    ...with Addgene's CRISPR Guide . Browse CRISPR plasmids available at Addgene through our CRISPR Plasmids and...tools like CRISPR-Cas9 are not only valuable but often essential. Find a collection of CRISPR plasmids ...Bacterial CRISPR Plasmids page . Additional Addgene Genome Engineering Resources Learn more about CRISPR with...zinc finger proteins. Addgene Blog CRISPR Methods for Bacteria CRISPR-based Genome Editing Systems For ...Empty Backbones Collection Molecular Biology Guide CRISPR Guide Bacteria are commonly used to create, store...coli INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons Return to top Credits Contributing ...

  14. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...efficiently in just a few steps. More recently, CRISPR technology has adapted Golden Gate cloning for ... you are using the Golden Gate method to create CRISPR/Cas9 constructs, assemble standard plasmids parts... Check out Addgene’s restriction cloning guide! CRISPR 101: Multiplex Expression of gRNAs Choosing a Molecular...

  15. Rett Syndrome

    Type
    Collection
    ...Addgene's CRISPR Guide for a general introduction to CRISPR technology or the mammalian CRISPR resources...'s Plasmids for Stem Cell Research page. CRISPR Tools CRISPR technology is a promising therapeutic approach...examples of additional CRISPR resources can be found below: Base Editing RNA Editing CRISPR-based DNA methylation...Resource Center MECP2 Animal Models Human Cell Lines CRISPR Tools Plasmids Resources The (Link opens in a new...

  16. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...increasing the size of the AAV donor template for CRISPR-mediated homology directed repairs (HDR). Split...sequential homology directed repairs (HDR) with CRISPR/Cas9. Donor A contains: 400bp homology arms to ...single step and serve as donors for two consecutive CRISPR-mediated HDR events which results in the integration....org/10.1172/jci34316 Bak RO, Porteus MH (2017) CRISPR-Mediated Integration of Large Gene Cassettes Using...

  17. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...proteins to monitor the localization of these genes, CRISPR to disrupt their function, and C. elegans overexpression...peritoneal mesothelioma tumorigenesis. They plan to use CRISPR and lentiviral plasmids from Addgene to knockout...

  18. Resolute Plasmid Collection

    Type
    Collection
    ...Insert Vector Type Industry PI Pooled Libraries CRISPR and overexpression pooled libraries are useful ...Human SLC Activation Library 132561 Superti-Furga CRISPR activation library targeting human SLC proteins...Human SLC Knockout Library 132552 Superti-Furga CRISPR knockout library targeting human SLC proteins. ...Mouse SLC Knockout Library 141436 Superti-Furga CRISPR knockout library targeting mouse SLC proteins. ...Knockout Libraries 213695 221409 221410 Superti-Furga CRISPR knockout library targeting human transmembrane ...
Showing: 281 - 300 of 452 results