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1. Visualizing Translation at the Single Molecule Level

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   Blog Post

   ...molecule level. The coding sequence of Yan et al.’s reporter contains 24 copies of SunTag, a synthetic...

2. The Power Behind NGS Plasmid Validation: seqWell

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   Blog Post

   ...tagging step. As an additional benefit of plexWell™’s sequential tagging procedure, the quantities of library...

3. Plasmids 101: Gram Negative and Positive Bacteria

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   Blog Post

   ...resources References Silhavy, TJ., Kahne, D. & Walker S. The Bacterial Cell Envelope. Cold Spring Harb Perspect...

4. Hot Plasmids and Viral Preps - September 2021

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   Blog Post

   ...DNA repair.  A new CRISPR pooled library targeting S. pneumoniae D39V from Jan-Willem Veening is now available...

5. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

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   Blog Post

   ...PI who generated the plasmid, followed by the PID(s) of your choice. Or if in doubt, copy and paste the...

6. Don’t Wait To Launch Your Health Science Startup

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   Blog Post

   ...Evan Spiegels of the world – individuals in their 20’s who’ve built multibillion dollar tech companies using...

7. Targeted Mutagenesis with EvolvR

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   Blog Post

   ... introduced to nCas9. These changes promote nCas9’s dissociation from DNA after nicking and yielded an...

8. Luminescent Imaging with Nano-lanterns

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   Blog Post

   ...multicolor luminescence imaging.” Proc Natl Acad Sci U S A 112(14) (2015): 4352-6. PMID: 25831507. PMCID: PMC4394297...

9. Antibodies 101: ChIP

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   Blog Post

   ...complexes with antibodies specific to your protein(s) of interest. Once the complex is captured, it is ...

10. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

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    Blog Post

    .... and Klebsiella spp. Dr. Devarshi Gajjar, The M. S. University of Baroda, India  Devarshi Gajjar has ...

11. Plasmids 101: Methylation and Restriction Enzymes

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    Blog Post

    ...sequence of the DNA surrounding your restriction site(s) can help you quickly predict which (if any) of those...

12. Ras Pathway

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    Collection

    ...targets. Rajalingam K, Schreck R, Rapp UR, Albert S. Biochim Biophys Acta. 2007 Aug;1773(8):1177-95. PubMed...

13. Control AAV Preps

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    ... 1, 2, 5, 8, 9, rg*, PHP.eB Roth 51506 AAV phSyn1(S)-tdTomato-WPRE hSyn tdTomato Constitutive 5 Zeng 58909...

14. Why and how to start studying chromatin (with Addgene's help)

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    Blog Post

    ...reagents. Yamagata M, Sanes JR Proc Natl Acad Sci U S A. 2018 Feb 13. pii: 1722491115. Addgene blog posts...

15. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    Blog Post

    ...reporter podcast segment Discovered in the early 1990’s, microRNAs (miRNAs) are recognized as important regulators...

16. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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    Blog Post

    ...Missinato, M. A., Onuoha, E., Perkins, L. A., Watkins, S. C., . . . Bruchez, M. P. (2016). A genetically targetable...

17. Screening for Successful Genome Editing with Digital PCR

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    Blog Post

    ...24509632. PubMed Central PMCID: PMC4063274. 2. Findlay, S. D., Vincent, K. M., Berman, J. R. & Postovit, L.-...

18. Hot Plasmids - February 2022

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    Blog Post

    ...single expression cassette is advantageous to Cas9’s larger expression requirements (Figure 1), but Cas12a...

19. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    Blog Post

    ...edition.  References and resources References Swift S, Lorens J, Achacoso P, Nolan GP. Rapid production ...

20. GCE4All: Making Genetic Code Expansion Accessible

    Type

    Blog Post

    ...come out! References Noren, C. J., Anthony-Cahill, S. J., Griffith, M. C., & Schultz, P. G. (1989). A General...