We narrowed to 441 results for: %s

1. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

   Type

   Blog Post

   ...more robust set of frankenbodies. References Ewert, S., Honegger, A., & Pluckthun, A. (2004). Stability ...

2. New and Upcoming Viral Vectors - September 2019

   Type

   Blog Post

   ...pENN.AAV.hSyn.Cre.WPRE.hGH (105553-AAV5) Dre vector AAV phSyn1(S)-DreO-bGHpA (50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo...

3. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

   Type

   Blog Post

   ...is widely used genome editing tool. However, Cas9’s endonuclease activity can be limited by the need for...

4. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

   Type

   Blog Post

   ...PI who generated the plasmid, followed by the PID(s) of your choice. Or if in doubt, copy and paste the...

5. In Vivo Biotinylation of Bacterial Fusion Proteins

   Type

   Blog Post

   ... non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized...

6. The Power Behind NGS Plasmid Validation: seqWell

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   Blog Post

   ...tagging step. As an additional benefit of plexWell™’s sequential tagging procedure, the quantities of library...

7. Visualizing Translation at the Single Molecule Level

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   Blog Post

   ...molecule level. The coding sequence of Yan et al.’s reporter contains 24 copies of SunTag, a synthetic...

8. Don’t Wait To Launch Your Health Science Startup

   Type

   Blog Post

   ...Evan Spiegels of the world – individuals in their 20’s who’ve built multibillion dollar tech companies using...

9. Antibodies 101: ChIP

   Type

   Blog Post

   ...complexes with antibodies specific to your protein(s) of interest. Once the complex is captured, it is ...

10. Targeted Mutagenesis with EvolvR

    Type

    Blog Post

    ... introduced to nCas9. These changes promote nCas9’s dissociation from DNA after nicking and yielded an...

11. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type

    Blog Post

    .... and Klebsiella spp. Dr. Devarshi Gajjar, The M. S. University of Baroda, India  Devarshi Gajjar has ...

12. Luminescent Imaging with Nano-lanterns

    Type

    Blog Post

    ...multicolor luminescence imaging.” Proc Natl Acad Sci U S A 112(14) (2015): 4352-6. PMID: 25831507. PMCID: PMC4394297...

13. Plasmids 101: Methylation and Restriction Enzymes

    Type

    Blog Post

    ...sequence of the DNA surrounding your restriction site(s) can help you quickly predict which (if any) of those...

14. Ras Pathway

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    Collection

    ...targets. Rajalingam K, Schreck R, Rapp UR, Albert S. Biochim Biophys Acta. 2007 Aug;1773(8):1177-95. PubMed...

15. Hot Plasmids - February 2022

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    Blog Post

    ...single expression cassette is advantageous to Cas9’s larger expression requirements (Figure 1), but Cas12a...

16. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    ...reporter podcast segment Discovered in the early 1990’s, microRNAs (miRNAs) are recognized as important regulators...

17. Early Career Researcher Toolbox: Social Media for Scientists

    Type

    Blog Post

    ... consistently engage with others about your topic(s) of interest. Hopefully through these links, I’ve:...

18. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type

    Blog Post

    ...edition.  References and resources References Swift S, Lorens J, Achacoso P, Nolan GP. Rapid production ...

19. Why and how to start studying chromatin (with Addgene's help)

    Type

    Blog Post

    ...reagents. Yamagata M, Sanes JR Proc Natl Acad Sci U S A. 2018 Feb 13. pii: 1722491115. Addgene blog posts...

20. Screening for Successful Genome Editing with Digital PCR

    Type

    Blog Post

    ...24509632. PubMed Central PMCID: PMC4063274. 2. Findlay, S. D., Vincent, K. M., Berman, J. R. & Postovit, L.-...