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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 868 results for: lic

Showing: 301 - 320 of 868 results

  1. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Plasmids from Collection ID Plasmid Description PI Publications Kaufman, M. L., Goodson, N. B., Park, K. U.,...

  2. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...include understanding of allelic variation between different plant species and metabolic properties affecting... ease of manipulation and their ability to be replicated. Because E. coli are easily propagated and have...minutes, they are perfect for molecular cloning and metabolic engineering.  The E. coli genome has an extensive...Escherichia coli as a Model Organism and Its Application in Biotechnology. In Escherichia coli - Recent...Physiology, Pathogenesis and Biotechnological Applications. InTech. https://doi.org/10.5772/67306 Koornneef...

  3. Tips for Getting a Faculty Position

    Type
    Blog Post
    ... do need multiple first/corresponding author publications in quality journals. It helps to come from the...a job. The currency of scientific success is publications and grants. 2. Write succinct and easy to understand...Attendees will include grad students (that are solicited for feedback), possibly the Dean, and even some...essential for human health. Failure to do so is implicated in several human diseases. Proper patterning ...disease treatment, etc., again in clear terms. "Implications for human disease" is vague. Instead, consider...interviews. For point of reference, I submitted my applications all by snail mail and only had phone interviews...

  4. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ..., the preferred vector, contains a promoterless splice acceptor-IRES-neoR gene, which enhances the efficiency...with a homologous repair template. It is this application - the creation of sequence-specific knockins ... allele of a single gene at once. Therefore, applications that require simultaneous modification of all...clones, making it unsuitable for most in vivo applications.  Third, even in the most optimized tissue-culture...efficiently deliver large repair templates for applications that require large gene fusions. The current...you’ve been able to check out some of the many applications of AAV in some of our other blog posts and have...

  5. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...exclude all duplicate events (two cells stuck together) from your single cells, as duplicates can’t be reliably...identify cells, the single cell gate to exclude duplicate events, and the Live/Dead gate to focus your analysis...cells, my marker stains of interest). Let’s double-click inside our gate and proceed to the next plot! Pro...show up in a straight line one the plot, while duplicates deviate from that pattern. A.                                                                                                         ... use H vs. A to distinguish single cells from duplicates. Lastly, you learned which gates should always...

  6. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...molecular biology applications for biotin and avidin. A short list of some other common applications and links...downstream application. Biotin labelled oligonucleotides can be used in a multitude of applications including...covalently attached to the active site of key metabolic enzymes including biotin carboxylase and decarboxylases... feng, Liu, N. hua, Shu, L. yuan, & Sun, H. “Application of avidin-biotin technology to improve cell adhesion...

  7. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...data, choosing controls, or mixing clear solutions—public speaking is skill that any scientist can learn....room, so it is a good idea to start thinking about public speaking early and often. Two of my jobs have indirectly... and note how they build suspense. Notice the simplicity of their slides and how the slides complement...of useful, entertaining science lab, career, and policy content. Watch Joanne give her "Not" Networking... done so. How can you expect to deliver a great public presentation if you are doing it for the first ...about how the sharing model works. See if you can elicit helpful comments from your audience as in “Does...

  8. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...reservoirs, chronic inflammation, and negative metabolic effects of ART. Why can’t the body mount an immune...create mutations in genes essential to HIV-1 replication. However, this approach could permit some viral...lessons learned in pursuing one technological application can benefit many others and accelerate the pace...: PMC4633726 Find SAM plasmids used in this publication at Addgene. Kaminski R, Chen Y, Fischer T, Tedaldi...and lentiviral packaging plasmids used in this publication at Addgene. Wang Z, Pan Q, Gendron P, Zhu W,...CRISPR/Cas9-Derived Mutations Both Inhibit HIV-1 Replication and Accelerate Viral Escape. Cell Rep. 2016 Apr...

  9. Resolute Plasmid Collection

    Type
    Collection
    ...development. RESOLUTE (Link opens in a new window) is a public-private partnership of 13 members from industry...

  10. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    ...DNA-binding domains" publication.  If you want to see this new feature in action, click here to explore the...'s Addgene ID # has been referenced in other publications, you'll find a link to the list of citing articles... what I mean. Additional features Once you've clicked on the "# References" link under the "Resource ... citing articles. From the article list you can click on the PubMed link to find the article abstract ...

  11. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...search, first pay attention to the validated applications listed on the vendor’s website and choose an...western and vice versa.  In addition to validated applications, pay close attention to the controls listed ...and incubation times. If the vendor includes publications that cited the antibody, consider reviewing ... the materials and methods sections of those publications.  Finally, always verify that the chosen antibody...choose antibodies that are validated for your application and test the antibody in your specific system...to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences...

  12. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...Figure 1. Used under Creative Commons Attribution License. Within the nervous system, remember that some...Creative Commons Attribution 4.0 International License.   Inducible promoters  This type of promoter.... A. (2023). Various AAV Serotypes and Their Applications in Gene Therapy: An Overview. Cells, 12(5), ...Anterograde Transsynaptic Tagging: Mapping Corticocollicular Input-Defined Neural Pathways for Defense ...Retrograde Serotype Viral Vectors 101: Viral Applications...

  13. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...additional origins of replication compared to monomers, they are also able to replicate at a higher frequency...the right, with the entire monomeric sequence duplicated in tandem, resulting in a plasmid that is twice...Characterization of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry...plasmid DNA production platform incorporating low metabolic burden seed-stock and fed-batch fermentation processes...What is a plasmid? Plasmids 101: Origin of ReplicationPlasmids 101: PCR...

  14. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...performed on one register at a time. If a particular application makes it difficult to isolate single registers...state read-out method may not be suitable for applications that require minimal cellular perturbation. ...The input plasmid (high copy, ColE1 origin of replication), contains large serine recombinase genes downstream...contains the register. Depending on the desired application, the inducible promoters we used to drive recombinase...program to find the one that best suits your application. How efficiently do RSMs work? We tested a two-input...anticipate that, depending on the context and application, some inducible recombinase systems may work ...

  15. Mammalian RNAi Tools

    Type
    Collection
    ...search our full site . ID Plasmid Vector Type PI Publication Additional Resources Addgene Resources pLKO.1...

  16. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...vesicular stomatitis virus G) are packaged as replication-defective virus particles with the SARS-CoV-2...

  17. CRISPR Plasmids and Resources

    Type
    Collection
    ...fused to deaminases can edit bases in RNA. Other Applications Screen CRISPR libraries are a powerful tool ...

  18. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ... plasmid or group of plasmids' functions and applications. We hope that these articles make it easier ...FLIM (Fluorescence Lifetime Imaging Microscopy) applications. To demonstrate the power of mCyFP1, the Lin... Coselection efficiency is so high that some applications may permit working with edited “pools” of cells...be found at Addgene and in Figure S12 of the publication. Agudelo D, et al. Nature Methods. 2017. PubMed...gels. They are shared through Addgene without licensing restrictions. Use these instructions to prepare... subtilis has a fully sequenced genome, many applications in biotech, and is considered the most studied...regulation to genome engineering that enables new applications, such as reversing the effects of dCas9 binding...

  20. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

    Type
    Blog Post
    ...Addgene ID number, your publication will be listed on our website as a “Publication Citing this Plasmid” ...upon other researchers being able to find their publications in the first place. As a curator of the thousands...make it easier for you to find the plasmids and publications relevant to your research interests. We do this...number as well as the reference for its original publication. To help facilitate this process, we explain ...
Showing: 301 - 320 of 868 results