We narrowed to 578 results for: multi
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TypeBlog Post...be beneficial when expression levels are low as multiple secondary antibodies can bind to a primary antibody...causing dead cells to fluoresce. When running a multicolor flow cytometry experiment, users must also prepare...
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Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...reprogramming factors can be delivered and expressed in multiple somatic cells via various methods. Some of the... of interest for in vivo transdifferentiation. Multiple cell types such as fibroblasts, hepatocytes, and... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog Post...conversion in principle, but some changes require multiple steps and enzymes. Prime editing can encode any... Multiple Authors... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog Post...modifications. CRISPR base editing persists through multiple cell divisions, indicating that this method produces... Multiple Authors... -
Plan Your Experiment
TypeGuide...different activator systems exist, including the multi-plasmid SAM system Activate Design and Clone Your...while testing multiple gRNAs is a good idea for most CRISPR experiments, testing multiple pegRNA designs... -
Protocol - How to Perform Sequence Analysis
TypeProtocol... you can see that just after base 70 there are multiple peaks in the same location. Looking at the trace... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...J., Deverman, B. E., & Gradinaru, V. (2020). Multiplexed Cre-dependent selection yields systemic AAVs ... -
Molecular Biology Protocol - Restriction Digest of Plasmid DNA
TypeProtocol...difficulty finding an enzyme that cuts your vector's multiple cloning site (MCS), but does not cut your insert... -
Protocol - Bacterial Transformation
TypeProtocol...transforming with very small amounts of DNA or if you're multiple plasmids at once. To save money, many labs also... -
Plasmids 101 eBook 4th Edition
TypeBlog Post...new sections, including our popular "Dimers and Multimers" post. It's also been re-organized and updated... -
The 12 Days of CRISPR: 2021
TypeBlog Post...CRISPR, Addgene gave to me: MUCICAT to perform multicopy chromosomal integration. On the tenth day of ... -
Addgene is Stopping Use of X (formerly Twitter)
TypeBlog Post... Multiple Authors... -
The Twelve Days of CRISPR
TypeBlog Post...the twelfth day of CRISPR, Addgene gave to me: multiplexed gRNAs. We hope you’ve been enjoying our CRISPR... -
Celebrating the 2024 Nobel Prize in Chemistry
TypeBlog Post...protein evolution. The Baker Lab has deposited multiple Top7 plasmids at Addgene, along with other tools... -
Bringing Renewable Energy to Addgene
TypeBlog Post... Addgene. The kitchen and office areas provide multiple streams for composting and recycling in the event... -
Antibody Data Repositories and Search Engines
TypeBlog Post...repository IBEX Knowledge-Base Any IBEX multiplex tissue imaging Data repository See blog post... -
An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success
TypeBlog Post...single gene) 26:00-28:39 : Methods for Delivering Multiple Genes to Cells for Gene Therapy 28:40-35:10 : ... -
Molecular Biology Reference
TypeGuide...sequencing”) comes in. NGS is a high-throughput, multi-parallel sequencing platform that can generate sequencing...combinations and only 20 natural amino acids, so multiple codons can encode the same amino acid — a phenomenon...providing a survival advantage to the bacterial host. Multiple Cloning Site (MCS) A short segment of DNA which... as cloned DNA, and this process of generating multiple identical copies of a recombinant DNA molecule...for Plasmid Selection Each bacterium can contain multiple copies of an individual plasmid, and ideally would...pressure to maintain and replicate your plasmid over multiple generations. It is important to distinguish that...puzzle pieces in only a few reactions. There are multiple approaches to acquire NGS, but one of the most... -
CRISPR Cheat Sheet
TypeBlog Post...library of gRNAs with a CRISPR nuclease target multiple genes in a cell population. The resulting mutant... -
Tips for Working in Industry & Success in Management: Interview with Crystal Shih
TypeBlog Post... Shih is an Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland....