We narrowed to 444 results for: crispr
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TypeBlog Post...therapy. SB is a valuable tool and an alternative to CRISPRs for genome engineering whose use is only likely...
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Botman-Teusink Yeast FP Collection
TypeCollection...Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Plasmids Bas Teusink Lab Materials... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog Post... first ever CRISPR-based clinical trial in 18 lung cancer patients. This study used CRISPR gene editing... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog Post...molecular biology protocols Find eBooks on plasmids, CRISPR, viral vectors, and more ... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog Post...Photosactivatable Fluorescent Proteins Use Light to Control CRISPR Genome Editing Choose the Brightest Fluorescent... -
Viral Vectors 101: Transductions
TypeBlog Post...integrate (unless you used a transposon system, CRISPR targeted strategy, etc.) and episomal expression... -
Plasmids 101: NGS Quality Control for Pooled Libraries
TypeBlog Post...Sequencing to Validate AAV Preps Learn More about Pooled CRISPR Libraries Resources on Addgene.org Browse Pooled... -
Synthetic Biology - Overview
TypeCollection...Orthogonal Switches Other Addgene Tools and Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit... -
CRISPR Guide
TypeGuide...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (3rd edition) CRISPR software and... Addgene Blog CRISPR topic page How to design your gRNA for CRISPR genome editing CRISPR 101: Homology.... Figure 1: Overview of the basic CRISPR mechanism Engineered CRISPR systems contain two components: a...generating gRNAs makes CRISPR one of the most scalable genome editing technologies, making CRISPR perfect for genome-wide... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...Lab Management Software for Biology Learn about CRISPR Pooled Libraries Resources at Addgene.org Find... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog Post...interactions with Alzheimer's, I found others using CRISPR to generate new knockout mice, using adenoviral... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog Post...are commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this more functional display... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post...genes for endogenous protein fusions created using CRISPR/Cas9. eNLs offer high luminescent signal for imaging... -
New and Upcoming Viral Vectors - May 2020
TypeBlog Post...now available as a lentiviral prep. It's a human CRISPR inhibition library that inhibits over 18,000 human...should be come available in the next few weeks. CRISPRi pooled library The Dolcetto pooled library is now... -
K. phaffii: Rising to the Occasion
TypeBlog Post...Resources on Addgene.org Yeast-mediated cloning CRISPR plasmids: Yeast Yeast Synthetic Biology Plasmids... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog Post...Resources Antibodies 101: Introduction to Antibodies CRISPR in the Clinic ... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog Post...determine if a variant is causal for a given disease. CRISPR has made genomic SDM relatively straightforward... -
Plan Your Experiment
TypeGuide... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan Your Experiment...you started using CRISPR in your research. Although we will use the example of CRISPR/Cas9 in mammalian...express the CRISPR machinery. For more difficult cells (e.g. primary cells), viral delivery of CRISPR reagents...conducting genome-wide screens using CRISPR AAV transduction CRISPR elements are inserted into an AAV transfer...lipids Transient expression of CRISPR components Short window of CRISPR activity may decrease off-target...Expression System Design gRNA Publications Get Started CRISPR is a powerful system that enables researchers to... cells, many of these principles apply to using CRISPR in other organisms. First, consider the genetic... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed to study the... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog Post...Blog Read the Plasmid 101 blog posts Check out CRISPR Meets Synthetic Biology: A Conversation with MIT...