We narrowed to 47 results for: Nov

The development of adenoviral vectors for the delivery of CRISPR/Cas9 expands genome engineering to primary cells.

Learn how repressible promoters work and how you can use them in your next experiment.

Long-read sequencing has allowed researchers to identify plasmid dimers and multimers. Learn about them - and how to troubleshoot - in this blog post.

The origin of replication is the DNA sequence which allows initiation of replication within a plasmid by recruiting transcriptional machinery proteins, enabling a plasmid to reproduce itself.

TOPO cloning is a restriction enzyme and ligase free cloning method. Learn how TOPO works in this post.

Exciting new plasmids, viruses, and antibodies in the Addgene collection!

Addgenie Eric Perkins curates Addgene resources you can use in your chromatin research.

Addgene's interviews Chris Voigt of MIT about the intersection of CRISPR and synthetic biology and his new work building circuits with CRISPRs.

Learn how to transform E. coli with your plasmid of interest.

Golden gate cloning can be used to efficiently and inexpensively clone multiple inserts into a single vector. Learn the basics of golden gate here.

Learn about a variety of AAV encoded calcium biosensors available from Addgene.

We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida serotype testing panel for the eye and brain.

REPAIR and RESCUE are two CRISPR tools for RNA editing based on Cas13b.

Learn how and why Adeno-associated virus (AAV) has enabled breakthroughs in retinal gene therapy.

Lentiviruses are useful for delivering DNA sequences to non-dividing mammalian cells. Learn all about the components of lentiviral vectors inside.

Addgene interviews Hodaka Fujii of Osaka University to learn about his lab's enCHIP system, their new CRISPR tools, community involvement, and more.

Find out more about the different homology directed repair pathways and how to design HDR templates for CRISPR editing.

Learn how to verify indels, deletions, and homology directed repair (HDR) events in your CRISPR modified cell lines.

Protospacer Adjacent Motifs (PAM) serve as a binding signal for Cas proteins. Here we highlight the many Cas proteins and their PAM sequence variations.

Scientists are currently developing disease treatments using CRISPR. Learn about recent developments in the fight against HIV-1 with CRISPR.