We narrowed to 33 results for: cas12a

1. Making CRISPR Plasmids Using Fragmid

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   ...selections.) CRISPR enzyme (Cas9 [sp], Cas9 [other], Cas12a, RNA-targeting) CRISPR mechanism (KO, CRISPRa...

2. Hot Plasmids and Viral Preps - January 2021

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   ...with a molecular weight roughly half of Cas9 or Cas12a. This is in part due to the fact that it only has...

3. Hot Plasmids - October 2020

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   ...CHyMErA) Knockout Libraries express hybrid Cas9-Cas12a gRNAs under a single U6 promoter. The paralog and...

4. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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   ...be useful for targeting ncRNAs. For example, the Cas12a variant can cleave single-stranded RNA using gRNAs...

5. Cpf1 Update: Comparison to Cas9 and NgAgo

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   ...Note: Cpf1 is also called Cas12a. In 2015, Feng Zhang’s lab characterized two Cpf1 nucleases, distant...

6. The AAVantages of AAV in CRISPR Screens

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   ...opt for a completely different Cas effector, like Cas12a (Cpf1). Even more creative options involve generating...

7. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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   ...developed nucleic acid detections tools based on Cas12a and Cas13  which could be repurposed for SARS-CoV...

8. Cpf1: A New Tool for CRISPR Genome Editing

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   ...Updated Aug 13, 2020. Note: Cpf1 is also called Cas12a. In 2015, Zetsche et al. added to the CRISPR toolbox...

9. How to Design Your gRNA for CRISPR Genome Editing

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   ...Cas enzyme, the development of SaCas9, NmeCas9, Cas12a enzymes, and engineered variants thereof offers...

10. Build Your CRISPR Vocabulary

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    ...appropriate species. Some Cas proteins, like LbCas12a or AsCas12a, do not require a tracrRNA. sgRNA: “Single-guide...

11. CasPEDIA: A Functional Classification of Cas Enzymes

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    ...dsDNA target in cis and has no trans activity. AsCas12a, on the other hand, generates staggered DNA nicks... Jinek et al. 2012, or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got you covered...

12. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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    ...researchers tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a, SpCas9-NG, SaCas9-KKH, CP1028-SpCas9, and...

13. Developing a cold tolerant E. coli using specialized chaperones

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    ...the applicability of our strategy using the pMBP-LbCas12a and pC013-Twinstrep-SUMO-huLwCas13a plasmids from...