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We narrowed to 27 results for: cas12a

Showing: 1 - 20 of 27 results

  1. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...screening toolkit by optimizing a Cas12a-based method for screens. Cas12a’s ability to perform multiplex editing...potential of Cas12a genetic screens by optimizing enzyme activity using an enhanced Cas12a variant (enAsCas12a...genes using Cas12a’s ability to knock out multiple genes in parallel. Lastly, this Cas12a-based screening...either Cas9 or Cas12a. Despite its small size, CasMINI is comparable in efficacy to dCas12a systems in both...targets Optimized genetic screening technique using Cas12a for multiplexed gene perturbation The Penn State...   Optimized genetic screening technique using Cas12a for multiplexed gene perturbation by: Gabrielle... larger expression requirements (Figure 1), but Cas12a has generally been underutilized in genetic screens...

  2. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ... RPA. When Cas12a-cRNA base pairs with the dsDNA of interest, the DNase activity of Cas12a is initiated...utilized CRISPR-Cas9 protein variants, Cas13, and Cas12a, to develop simple, portable, and inexpensive platforms... 2017 and 2018). While the Doudna lab has used Cas12a’s non-specific ssDNA degradation to develop the ...degradation of neighboring ssRNA and ssDNA by Cas13 and Cas12a, respectively, to cleave and activate a reporter...reaction, Cas enzymes, such as variations of Cas13 and Cas12a, are combined with different fluorescent reporters...DNA Endonuclease Targeted CRISPR Trans Reporter Cas12a (Cpf1) is a CRISPR Cas variant that can also cleave...dsDNA similarly to Cas9 (Zetsche et al., 2015 ). Cas12a however recognizes a different PAM site and generates...

  3. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...different Cas12a inhibitors. Some of these anti-Cas12a proteins have broad inhibitory effects on Cas12a orthologs...the Mobile-CRISPRi plasmids Identification of Cas12a inhibitors Using a bioinformatics and a screening...orthologs and can block Cas12a-mediated genome editing in human cells. Read the paper in Science Find...

  4. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ... AAG or TTC  Best for: very large deletions.   Cas12a – The staggered cutter  Originally classified as...generates staggered breaks, also known as sticky ends. Cas12a cleavage results in 5 bp overhangs and is particularly...DNA generation, such as homology directed-repair. Cas12a is also conducive for targeting AT-rich genomic...shared by other Cas enzymes outlined below as well. Cas12a also has significant therapeutic promise due to...pre-crRNA into crRNA). The size and simplicity of Cas12a makes it easier to package for therapeutic delivery...cutting efficiency and diversify PAM sequences of Cas12a to further optimize it for therapeutic and laboratory...size than traditional Cas9. Another similarity to Cas12a is that Cas14 also indiscriminately cleaves non-complementary...

  5. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...of SARS-CoV-2. If the Cas12a-sgRNA complex binds to the target sequences, Cas12a activity gets activated...Unlike Cas12a, Cas13a does not require strict sequence preferences at the target site, while Cas12a requires..., check out this table.  All-in-one dual CRISPR/Cas12a (AIOD-CRISPR) assay – for rapid, ultrasensitive...reaction system and incubated at one temperature. Two Cas12a-crRNA complexes are designed, each binding to one...cleavage. Upon amplification by the RPA primers, the Cas12a-crRNA binds to the complementary region in the ...the ssDNA-FQ reporter resulting in fluorescence. Cas12a binds all the amplified target regions and results...

  6. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...CRISPR protein adapted for genome engineering was Cas12a (formerly Cpf1), a nuclease that generates double...ends” rather than the blunt ends created by Cas9. Cas12a  displays lower off-target editing than SpCas9 ...Kim et al., 2016).  The Acidaminococcus sp. BV3L6 Cas12a, AsCpf1, uses a 5'-TTN PAM which makes it easier...

  7. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...first thought to enable MBE by using Cas12a fused base editors (dCas12a-BE). However, the poor editing efficiencies...only minimal payloads. DAP array  Although CRISPR-Cas12a, with abilities to process and release its own ...

  8. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...Additionally, alternative Cas enzymes exist, such as Cpf1/Cas12a, which recognizes the PAM sequences of TTTV. While... sequence  Additional notes SpCas9 NGG - Cas12a TTTV Great for AT-rich regions Cas9 EQR NGAG...

  9. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ... It can be! Cas12a is a Cas family member which generates sticky ends upon cutting. Cas12a may promote...

  10. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...cancer research. The Mouse Metastasis CRISPR-Cpf1(Cas12a) Double Knockout Library is a lentiviral library...

  11. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...simultaneous gene activation in plants.  FnCas12a, unlike other Cas12a nucleases identified, functions at temperatures...

  12. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    .... elegans. In 2017, the Korswagen lab developed Cas12a (Cpf1) for editing in C. elegans (Ebbing et al....motif required by the Acidaminococcus sp. BV3L6 Cas12a (AsCpf1) eases this constraint and allows editing...

  13. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...system is less than half the size of the Cas9 or Cas12a based systems, making it well suited for AAV packaging...

  15. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...selections.) CRISPR enzyme (Cas9 [sp], Cas9 [other], Cas12a, RNA-targeting) CRISPR mechanism (KO, CRISPRa...

  17. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...CHyMErA) Knockout Libraries express hybrid Cas9-Cas12a gRNAs under a single U6 promoter. The paralog and...
Showing: 1 - 20 of 27 results