We narrowed to 30 results for: cas12a

Our February hot plasmids, including two new CRISPR tools, a protein ladder, and anti-FLAG frankenbodies.

Learn how CRISPR-based diagnostic systems can easily detect nucleic acids of choice with high sensitivity, specificity and portability.

This blog series showcases some of the new CRISPR tools we have at Addgene. Read about CRISPR-Cas12b for human genome editing, plant base editors, string assembly gRNA cloning, lineage tracing tools, and more.

Comparing and contrasting Cas9 to the other genome editing-capable Cas family proteins.

Learn about TnpB, a small genome editor with a function similar to CRISPR systems!

High schoolers invented a new diagnostic for Lyme disease. In the process, they also created a tool for jointly designing RPA primers and Cas12a sgRNAs.

With the limitations of qRT-PCR based COVID-19 testing, scientists develop CRISPR-based diagnostic tools for COVID-19.

Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.

Protospacer Adjacent Motifs (PAM) serve as a binding signal for Cas proteins. Here we highlight the many Cas proteins and their PAM sequence variations.

Drive-and-proccess (DAP) arrays are a new tool from the Gao lab that allows for up to 31-loci CRISPR multiple base editing.

Browse Addgene’s curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community forums.

A review of alternative PAM and PAM-flexible Cas enzymes.

Learn how you can use HDR and CRISPR to make a knock-in cell line for your research.

Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.

Learn about CRISPR plasmids at Addgene such as an E. coli genome-wide CRISPRi library, GeneWeld vectors, CasX plasmids, and drug inducible CRISPR/Cas9 activation.

Recent highlights from Addgene's collection include CRISPR-based editing in organoids and Biosensors for detecting strigolactones.

Recent developments using CRISPR/Cas9 editing with C. elegans, focusing on the use of an embryonic lethal selection marker to select for knock-in.

This post discusses the applications, advantages, and caveats to using hybrid guide RNAs, in which DNA bases are substituted for RNA bases.

Learn about the latest CRISPR plasmids deposited at Addgene, including a miniature CRISPR-Cas systems, SEND plasmids, and prime editing improvements

With it's small crRNAs and RNase activity, Cpf1 can easily be used for multiplex genome editing. Learn more about new multiplex Cpf1 tools here.