CRISPR Guide
Type
Collection
... with Cas12a One of the most popular Cas9 alternatives is Cas12a (Cpf1) . There are many Cas12a orthologs...benefits. Cas12a exhibits increased efficiency with larger edits using HDR. Cas12a-mediated DNA cleavage...common is AsCas12a (from Acidaminococcus sp. ). The popularity can be attributed to Cas12a’s many benefits...versions of Cas12a recognize different PAM variants, with one of the most popular being EnCas12a, an engineered...of Cas9 exist, along with other Cas enzymes like Cas12a, which are available for specialized applications...appropriate Cas enzyme. Specific enzymes, such as Cas12a or Cas13 , can improve multiplexing efficiency ... particular type of Cas enzyme, such as Cas9 or Cas12a. These are identified in their names — AcrII proteins...