We narrowed to 32 results for: gibson assembly

1. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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   ...gene of interest into the FP-SEC vector using Gibson assembly. This construct will serve as the repair template...

2. The time and cost required to make a plasmid

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   ...budget is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’ll have...

3. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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   ...ligase-mediated method, the more recently developed Gibson Assembly Cloning and Gateway® cloning technologies, ...

4. When Fidelity Matters: A frank discussion about ligase fidelity

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   ...example, DNA assembly methods, such as Gibson Assembly® and NEBuilder® HiFi DNA Assembly, require nick-selective...Plasmid Cloning Techniques Pushing the Limits of DNA Assembly Plasmid Cloning by PCR Resources on Addgene.org...

5. Plasmids 101: Colony PCR

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   ...Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional Resources on Addgene.org Read ...

6. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

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   ...extra elements, like a repair template, using Gibson Assembly or restriction enzyme cloning. Streptomyces...are easily inserted using BbsI and Golden Gate Assembly. Either plasmid can be also linearized with XbaI...Microbiome Bacteria with a Toolkit for Modular Assembly of Broad-Host-Range Plasmids. ACS Synth Biol 7...

7. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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   ... recommend using overlap extension PCR and Gibson assembly to piece together the registers. All parts ...

8. Cloning

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   ...exonuclease. Gibson Assembly (Image from Plasmid 101: Gibson Assembly ) Back to Top Type IIS Assembly (Golden...post on TOPO cloning. Back to Top Gibson Assembly (Isothermal Assembly Reaction) Isothermal cloning, more...more commonly known as Gibson assembly ( protocol ), takes advantage of the properties of 3 common molecular... DNA ligase fuses DNA strands together. In Gibson assembly DNA fragments with 20-40 base pair homology...DNA. A major benefit of Gibson cloning is that it allows for the simple assembly of multiple fragments ...cloned into. A drawback of this system is that Gibson assembly works best when combining DNA fragments over...Engineering Guide Restriction Enzyme Gateway TOPO Gibson Type IIS Ligation Independent Cloning Oligo Stitching...

9. Adenovirus Guide

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   ...cloning methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have...primarily encode for structural proteins needed to assemble icosahedral capsids and build new virions. Figure...

10. Gamma-Retroviral Vector Guide

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    ...cloning methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have...

11. Adeno-associated virus (AAV) Guide

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    ...cloning methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have...and Cap regions necessary for the production and assembly of viral capsids. Helper plasmid — containing ...rAAV vector (∼5%). Gene fragments can also be reassembled via homologous recombination, when both sequences...

12. Lentiviral Vector Guide

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    Guide

    ...cloning methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have...