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We narrowed to 47 results for: lox- cre

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Showing: 21 - 40 of 47 results

  1. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...(orange) (Livet et al., 2007). Brainbow uses a Cre/lox system to label neurons. Genes encoding the fluorescent...fluorescent protein genes, Cre recombination is required before fluorescence can occur. Cre recombinase can be...developing fish via Cre protein microinjection or crossing of Zebrabow animals to an inducible Cre line. The Schier...gene directly following the promoter is expressed. Cre-mediated deletion of one or several of these genes...system. One such system is Brainbow 2.0, which uses Cre to trigger the inversion of two genes aligned head-to-head...head-to-head. Another variation, Brainbow 2.1, uses Cre to trigger the inversion of two tandem sets of genes...when crossed with mice expressing the appropriate Cre allele. The Confetti mouse contains the Brainbow ...

  2. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...using Cre-Lox mouse models in which the endogenous MECP2 gene was disrupted and silenced by a Lox-Stop ...excision of the Stop cassette using tamoxifen-inducible Cre not only halted the neurological damage but completely...

  3. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...control when you turn off expression for good, the Cre-Lox system offers inducible yet permanent gene excision...can be excised by intentional introduction of the Cre recombinase. This is a time-tested system but does...some permanent knock out plasmids? Check out our Cre vector and Murine LoxP vector.   Turn it off for ...

  4. Rabies and Neuronal Tracing

    Type
    Blog Post
    ... For example, a researcher can deliver Cre-dependent TVA to Cre-expressing neurons in a transgenic mouse...Gene Delivery to the CNS and PNS Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist's View Control Gene...viral protein EnvA on its surface will only infect Cre-positive, TVA-expressing cells (Osakada et al., 2013...

  5. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...tool for marking and tracking cells is the the Cre/lox recombination system. A researcher can flank fluorescent...respectively. The permanent labelling of cells using cre-lox is advantageous when compared to photoactivable...expression is turned on or off in cells expressing Cre. The result of a system like this is a mosaic of ...

  6. Important Considerations When Using AAVs

    Type
    Blog Post
    ...system for controlling gene expression such as Cre-lox, may be sufficient to overcome the transport issues... to produce neuron specific transfection using Cre-lox, this new method could allow for labeling of neurons...

  10. Recombinases AAV Preps

    Type
    Collection
    ...expression. See our Cre-lox Plasmids page for more information about these molecular tools. Cre AAV ID Name Promoter...pAAV-Ef1a-mCherry-IRES-Cre EF1a mCherry (not a fusion tag) 8, rg* Karl Deisseroth 55636 pAAV-EF1a-Cre EF1a none 11...Wilson 24593 AAV-pgk-Cre PGK none rg* Patrick Aebischer 51507 AAV pmSyn1-EBFP-Cre Syn EBFP 5 Hongkui Zeng...107312 AAV-hSyn-mCherry-P2A-Cre-WPRE Syn mCherry 1 Hui Yang 107738 pAAV-hSyn-Cre-P2A-dTomato Syn dTomato ...Recombinases Viral Vector Packaging Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible Recombinases... 11, rg* Karl Deisseroth 121675 pAAV-EF1a-fDIO-Cre EF1a none 1, 5, 8, 9, rg* Esteban Engel , Alexander...none 5, PHPeB James M. Wilson 196410 AAV-GfaABC1D-Cre-4x6T GfaABC1D none 5 Stanley Thomas Carmichael 214848...

  12. E11 Bio PRISM Collection

    Type
    Collection
    ...neuron subtypes in Cre-driver lines. These could also be used in conjunction with Cre-enhancer viruses ...Other Markers AAV Packaged on Request AAV Guide Cre-Lox Plasmid Collection Content last reviewed: 5 September...242781), but E11 Bio has also developed a set of Cre-dependent, hSyn-Tet inducible plasmids (Addgene #242782...

  13. Common Injection Routes in Mice

    Type
    Blog Post
    ...Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox plasmids References Prathipati, P., Rodriguez-Aguayo...

  15. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Expression Vectors page Cre-lox Site-specific recombination See our dedicated Cre-lox Plasmids page pBF3060...including plasmids for constitutive, conditional (Cre-lox), or inducible (Tet) expression miRNA and 3' UTR...

  16. Mammalian RNAi Tools

    Type
    Collection
    ...expression as well as those that allow for conditional (Cre-lox) or inducible (Tet) expression are available. To...Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more. Plasmid...

  17. Plasmid Collections

    Type
    Collection
    ...Engineering Genome Engineering Overview CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent Proteins...

  18. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...considered safe for clinical applications. Excisable Cre-lox lentiviral vectors: This approach is the same as...formation in iPSC-derived and iPSC-transplanted mice. Cre-deletable or Tet-inducible lentiviruses address some...standard lentiviral vector, but transient expression of Cre recombinase allows for deletion of the inserted transgenes...

  19. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ... involving recombination elements such as FRT or Lox sites, and provides a modular system for easier exchange... short hairpin) and CRUSH (Conditional RUSH) use Cre-mediated recombination to turn on or off the expression...a dual-color RNAi “sensor” mouse strain in which Cre expression causes the induction of both DsRed fluorescence...Mice carrying the R26DsRedR; CRUSH-GFP; and Nestin-Cre alleles showed efficient GFP knockdown in eyes and... recombination events at the genetic level. Upon Cre/loxP recombination, each transgene expresses one ...Flp recombinase/FRT system in place of Brainbow’s Cre/loxP, allowing for simultaneous use of the two systems...SUMO tags in the FP sequence for separation from Cre-based Brainbow-expressing cells. Autobow plasmids...

  20. Deisseroth INTRSECT Collection

    Type
    Collection
    ...of populations expressing only Cre AND Flp, Cre NOT Flp, or Flp NOT Cre. B) This strategy has been expanded...EYFP Cre AND Flp Yes 231926 pAAV-Ef1a-Con/Fon-EYFP Cre AND Flp No 55651 pAAV-hSyn Con/Foff EYFP Cre AND...AND NOT Cre Yes 137129 pAAV-Ef1a-Con/Fon-BFP Cre AND Flp Yes 137130 pAAV-Ef1a-Con/Foff 2.0-BFP Cre AND NOT...expressing Cre AND Flp, here expressed through a double transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp...Viral Items 55636 pAAV-EF1a-Cre None Yes 55632 pAAV-Ef1a-mCherry-IRES-Cre None Yes 55637 pAAV-EF1a-Flpo.../Foff 2.0-EYFP Cre AND NOT Flp FRT/F5 Yes 55652 pAAV-hSyn Coff/Fon EYFP Flp AND NOT Cre No 231927 pAAV-Ef1a-Coff...pAAV-Ef1a-Coff/Fon-BFP Flp AND NOT Cre Yes 137132 pAAV-Ef1a-Con/Fon-mCherry Cre AND Flp Yes 137133 pAAV-Ef1a-Con...
Showing: 21 - 40 of 47 results