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  2. The Twelve Days of CRISPR

    Type
    Blog Post
    ..., Addgene gave to me: five methods for detecting SARS-CoV-2 using CRISPR.  On the sixth day of CRISPR,...

  4. An Introduction to Adenovirus

    Type
    Blog Post
    ...to develop that this illness was caused by either SARS-CoV-2, Adenovirus type 41 (AdV-41), or Adenovirus-Associated...unusual cases was either 1) a physiological effect of SARS-CoV-2 toward destabilizing one’s natural immune ...: 10400791. Kafri, T., Morgan, D., Krahl, T., Sarvetnick, N., Sherman, L., & Verma, I. (1998). Cellular...

  10. Summer SciComm: Preprints

    Type
    Blog Post
    ...researchers to share information about the emerging SARS-CoV-2 virus quickly and openly. By sharing results...bioRxiv. https://doi.org/10.1101/2021.09.07.459259 Sarabipour S, Debat HJ, Emmott E, Burgess SJ, Schwessinger...

  11. Hot Plasmids - August 2020

    Type
    Blog Post
    ...CRISPR-Cas13 based antiviral method to degrade RNA from SARS-CoV-2 sequences and live influenza A virus. It was...

  12. Bacterial Expression Systems

    Type
    Collection
    ...promoter repressed by EsaR. Designed to be used with the pAC-EsaR Series . pAC-EsaR Series Various Plasmids...transcription factors, and reporter genes. pCS-PesaRlux 47640 PesaR 3OC6HSL (Quorum sensing molecule) Cynthia...inducible expression of EsaR, a transcription factor responsive to 3OC6HSL. EsaR derivatives found herein...sensitivities to 3OC6HSL. The EsaR-responsive promoter can be found in pCS-PesaRlux controlling luciferase expression...

  13. Plan Your Experiment

    Type
    Collection
    ...First, consider the genetic manipulation that is necessary to model your specific disease or process of interest...specific application, and in such cases, it may be necessary to customize an existing reagent to suit your ...cells Packaging and envelope plasmids provide the necessary components to make lentiviral particles (for details...ideally less than 10 bp away. In this case, it is necessary to identify the exact location where the edit ...-target activity A PAM sequence is absolutely necessary for Cas9 to bind target DNA. As such, one can ...Before proceeding with your experiment, it may be necessary to optimize your delivery conditions. Learn more...your desired edit has occurred? The exact method necessary to validate your edit will depend upon your specific...

  14. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...skeletal type Sarcomeric thin filaments 114412 TTN-mEGFP AICSDP-45 mEGFP Titin Sarcomeres (M-line tag) ...mEGFP MLC-2a Sarcomeric thick filaments 114414 MYL2-mEGFP AICSDP-49 mEGFP MLC-2v Sarcomeric thick filaments...Cytoplasm 114405 ATP2A2-mEGFP AICSDP-41 mEGFP SERCA2 Sarcoplasmic reticulum/endoplasmic reticulum 114406 SMC1A-mEGFP...124607 ACTN2-mEGFP AICSDP-63 mEGFP Alpha-actinin-2 Sarcomeric z-disks 124608 NPM1-mTagRFP-T AICSDP-69 mTagRFP-T...

  15. Immunology Research Plasmids and Resources

    Type
    Collection
    ...member 2 BXR, ONR1, PAR, PAR1, PAR2, PARq, PRR, PXR, SAR, SXR NR1I3 nuclear receptor subfamily 1, group I,...CD32B, FCG2, FCGR2, IGFR2 FOS v-fos FBJ murine osteosarcoma viral oncogene homolog AP-1, C-FOS GSK3B glycogen...synthase kinase 3 beta - HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1...oncogene AP-1, AP1, c-Jun KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog C-K-RAS, K-RAS2A, K-RAS2B...3, LIR3, MGC138403, PIRB LYN v-yes-1 Yamaguchi sarcoma viral related oncogene homolog FLJ26625, JTK8 MALT1...platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) FLJ12858, PDGF2... death. Symbol Name Synonyms ARAF v-raf murine sarcoma 3611 viral oncogene homolog A-RAF, ARAF1, PKS2,...

  16. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...membrane voltage with improved brightness (QuasAr1 and QuasAr2) All-optical electrophysiology in mammalian...in C. elegans (Arch(D95N), MacQ-mCitrine, QuasAr and QuasAr-mOrange) Rhodopsin-based voltage imaging tools...stress on cells. Nat Commun. 2021 Sep 14;12(1):5438. Cesar Cuevas-Velazquez , Jose Dinneny Lysosomal pH Lyso-pHluorin...Campbell Voltage Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters...

  17. CRISPR Guide

    Type
    Collection
    ...detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar to Cas9 and...Plan Your Experiment Web References PAM Sequences Glossary Publications CRISPR Overview Bacteria have an ...synthetic RNA composed of a scaffold sequence necessary for Cas-binding and a user-defined ∼20-nucleotide...collection are from S. pyogenes unless otherwise noted. Glossary Term Definition Base editor Fusion of a Cas protein...Sequence adjacent to the target sequence that is necessary for Cas enzymes to bind target DNA PCR P olymerase...551 (7681), 464–471. PMID: 29160308 Hess, G. T., Frésard, L., Han, K., Lee, C. H., Li, A., Cimprich, K. ...L., Liu, Z., Brar, G. A., Torres, S. E., Stern-Ginossar, N., Brandman, O., Whitehead, E. H., Doudna, J...

  18. AAVED

    Type
    Collection
    ... Laboratory Gowan Tervo Janelia Research Campus Sarada Viswanathan Janelia Research Campus Kenton Woodard...10:15 AM AAV Retrograde Tracing Gowan Tervo and Sarada Viswanathan 11:00 AM Circuit Tracing Ali Cetin ..., Cold Spring Harbor Laboratory Gowan Tervo and Sarada Viswanathan, Janelia Research Campus Kenton Woodard...

  19. Arf GTPase Family

    Type
    Collection
    ...pcDNA3), Gateway GTPase Sar1a 56681 198 Bacterial (pET), Gateway GTPase Sar1b 51128 198 Gateway GTPase...

  20. Ras Pathway

    Type
    Collection
    ...Farnesyltransferase, CAAX box FOS FBJ murine osteosarcoma viral oncogene homolog GRB2 Growth factor receptor...exchange factor RAS KRAS MRAS NRAS Kirsten rat sarcoma viral oncogene homolog Muscle RAS oncogene homolog...
Showing: 21 - 40 of 324 results