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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 24 results for: vsv-g

Showing: 21 - 24 of 24 results

  1. Lentiviral Vector Guide

    Type
    Guide
    ...gene env (usually VSV-G due to wide infectivity) VSV-G (vesicular stomatitis virus G protein) is the most...Interchangeable; usually encodes for VSV-G Interchangeable; usually encodes for VSV-G Safety Safe; replication incompetent...gene; typically vesicular stomatitis virus G glycoprotein (VSV-G), an envelope protein with broad tropism...the envelope gene, a process called pseudotyping. VSV-G is by far the most common envelope gene, as it has... the stability of the viral particles. However, VSV-G is toxic under long-term, constitutive expression...expression. In order to make stable cell lines, VSV-G is often put under an inducible promoter. Depending on ...doi.org/10.3390/v3020132 PMID: 22049307 Ghaleh, H. E. G., Bolandian, M., Dorostkar, R., Jafari, A., & Pour...

  3. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... any nucleotide, R is a purine (A,G), and Y is a pyrimidine (C,U). G-C content should be 36-52%. Sense....3 Protocol for lentiviral infection and selection G. Safety H. References H.1 Published articles H.2 Web...Packaging plasmid for producing viral particles. pMD2.G Envelope plasmid for producing viral particles. Note...Material Vendor and catalog # psPAX2 Addgene #12260 pMD2.G Addgene #12259 HEK-293T cells GenHunter: #Q401 FuGENE...plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid to 20 μl serum-free OPTI-MEM TIP:.... Incubate for 20-30 minutes at room temperature. g. Retrieve HEK-293T cells from incubator. The cells...positive control for the puromycin selection. Days 4+: g. Change to fresh puromycin-containing media as needed...

  4. Molecular Biology Reference

    Type
    Guide
    ... A, G, or T H A, C, or T K G or T M A or C N A, T, C, or G R A or G S C or G V A, C, or G W A or T Y C...) and Guanine (G). In the double helix A always pairs with T and C always pairs with G. RNA on the other... Cytosine G Guanine T Thymine U Uracil Single Letter Code: Ambiguous bases Nucleobase B C, G, or T D A...cytosine, and guanine (abbreviated to A, T, C, and G respectively) that are organized into a double stranded...strand. Specifically, A pairs with T and C pairs with G. During replication, DNA unwinds and the DNA polymerase... DNA strand in addition to the normal A, T, C, or G nucleotide. The 4 standard bases are tagged with a...time a base is added. Again, each base (A,C,T, or G) is labelled with a different color making it easy...
Showing: 21 - 24 of 24 results