We narrowed to 432 results for: %s
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TypeBlog Post... can be used across species, for example, in both S. cerevisiae and E. coli. Auxotrophic selection is ...
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Transferable Skills Guide: Career Planning Resources
TypeBlog Post...20140417174121-34888774-linkedin-etiquette-guide-20-do-s-don-ts Resources for getting into grad school: https... -
DNA Purification Without a Kit
TypeBlog Post... Y., He, W., Wang, J., Peng, F., Huang, L., Zhao, S., & Deng, W. (2018). Rapid Regeneration and Reuse ... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post... --very-sensitive-local -L 9 -N 1 -U GRNA_23MERS -S GRNA_HITS.sam Many thanks to our guest blogger, Aneesh... -
Plasmids 101: Control Plasmids
TypeBlog Post...decreased expression of Gene X. Ergo the positive control(s) should decrease the expression of Gene X. Since the... -
Tips for Using FRET in Your Experiments
TypeBlog Post.... 2007 Sep; 32(9): 407-14. Zimmermann T, Terjung S. FRET Basics and Applications: An EAMNET Teaching ... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog Post...interest will be expressed. The RNA polymerase(s) RNA is transcribed from DNA using an RNA polymerase... -
Negotiating Work and Life: How to Find the Joy
TypeBlog Post... not to do this. For a family with working parent(s), the sooner your kids can make a decent meal, fold... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post...trouble by confirming your transfer worked with Ponceau S staining or other reversible protein staining immediately...resources References Luo, H., Rankin, G., Straley, S., & Chen, Y. (2011). Prolonged Incubation and Stacked... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog Post...Foronda M, Zimmerman J, Diaz B, Zafra MP, Goswami S, Dow LE (2020) GO: a functional reporter system to... -
Transferable Skills Guide: Creativity
TypeBlog Post...mental-mistakes-that-kill-your-productivity Finkelstein, S. (2019, July 15). A brief exercise to spur innovation... -
Hot Plasmids Spring 2024
TypeBlog Post...many closely-packed labeled cells. Plus, Voltron2’s fluorescence is tunable by changing the dye that becomes... -
QC Sequencing Technologies at Addgene
TypeBlog Post...//doi.org/10.1021/ac2010857 Sanger, F., Nicklen, S., & Coulson, A. R. (1977). DNA sequencing with chain-terminating... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post...2958.2005.04643.x Pubmed PMID: 15916596 Kamruzzaman M, Shoma S, Thomas CM, Partridge SR, Iredell JR (2017) Plasmid... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...Many thanks to our guest blogger Gary McDowell! Gary S. McDowell is currently the Executive Director of Future... -
Site Directed Mutagenesis by PCR
TypeBlog Post...minor extension can usually ensure that the 3’-base(s) do not form secondary structures. The introduction... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post... tactic, and puts you ahead of most start-up P.I.’s. It doesn’t have to be complicated, or difficult to... -
27 Hot Plasmids from 2016
TypeBlog Post...PMID: 25895059 Barnea, et al. Proc Natl Acad Sci U S A. 2008. PubMed PMID: 18165312 CIDAR MoClo parts ...or clusters. Veggiani, et al. Proc Natl Acad Sci U S A. 2016. PubMed PMID: 26787909 EasyClone 2.0 Yeast...Hanson SM, Rodríguez-Laureano L, Albanese SK, Gradia S, Jeans C, Seeliger MA, Levinson NM, Chodera JD. bioRxiv... -
Luciferase Plasmid Collection
TypeCollection... in mammalian systems. ID Plasmid Luciferase Type(s) Description PI 64784 pGL3-Basic-IRES Firefly Insertion... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog Post...antibiotics! References Espah Borujeni, Amin, Anirudh S. Channarasappa, and Howard M. Salis. "Translation ...