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Showing: 381 - 400 of 439 results
  1. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ... can be used across species, for example, in both S. cerevisiae and E. coli. Auxotrophic selection is ...
  2. DNA Purification Without a Kit

    Type
    Blog Post
    ... Y., He, W., Wang, J., Peng, F., Huang, L., Zhao, S., & Deng, W. (2018). Rapid Regeneration and Reuse ...
  3. MXS Chaining

    Type
    Blog Post
    ...: PMC2822740. 2. Engler C, Kandzia R, Marillonnet S. A one pot, one step, precision cloning method with...
  4. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...attL-entry-TOPO vector. TOPO cloning adds short end(s) to facilitate cloning into an attL-containing entry...
  5. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...decreased expression of Gene X. Ergo the positive control(s) should decrease the expression of Gene X. Since the...
  6. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...trouble by confirming your transfer worked with Ponceau S staining or other reversible protein staining immediately...resources References Luo, H., Rankin, G., Straley, S., & Chen, Y. (2011). Prolonged Incubation and Stacked...
  7. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...minor extension can usually ensure that the 3’-base(s) do not form secondary structures. The introduction...
Showing: 381 - 400 of 439 results