We narrowed to 441 results for: %s

1. New Tools Enable CRISPRa for Neuroscience Applications

   Type

   Blog Post

   ...lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (vector 1) with neuron-specific expression...

2. Isolating Genomic Regions of Interest with the CRISPR System

   Type

   Blog Post

   ...(2017): 1028-1043. PubMed PMID: 28841410. Qi, Lei S., et al. "Repurposing CRISPR as an RNA-guided platform...

3. Hot Plasmids - November 2023

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   Blog Post

   ... Find PE6 plasmids here!  Doman, J.L., Pandey, S., et al. (2023). Phage-assisted evolution and protein...

4. How to Be an Excellent Trainee

   Type

   Blog Post

   ...days or weeks), look over your notes and protocol(s). Is there enough information there for you to be ...

5. Viral Vectors 101: Preparing Pooled Libraries

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   Blog Post

   ...10.1002/cpmb.8. PMID: 27366891. Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD...

6. Getting to Know Expansion Microscopy

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   Blog Post

   ...992. https://doi.org/10.1038/nbt.3625  Truckenbrodt S, Sommer C, Rizzoli SO, Danzl JG (2019) A practical...

7. Plasmids 101: Shuttle Vectors

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   Blog Post

   ... can be used across species, for example, in both S. cerevisiae and E. coli. Auxotrophic selection is ...

8. Great Results Start with Great Standard Curves

   Type

   Blog Post

   ... standard, standard dilutions, and unknown sample(s) in the same buffer if possible, or very similar buffers...

9. Plasmids 101: Codon usage bias

   Type

   Blog Post

   ...well correlated with codon bias in both E. coli and S. cerevisiae. Protein folding - If a protein is encoded...

10. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    ...the pre-designed primers to determine which version(s) of the capsid gene successfully infected the cell...

11. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    ... --very-sensitive-local -L 9 -N 1 -U GRNA_23MERS -S GRNA_HITS.sam Many thanks to our guest blogger, Aneesh...

12. Plasmids 101: Control Plasmids

    Type

    Blog Post

    ...decreased expression of Gene X. Ergo the positive control(s) should decrease the expression of Gene X. Since the...

13. Tips for Using FRET in Your Experiments

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    Blog Post

    .... 2007 Sep; 32(9): 407-14. Zimmermann T,  Terjung S. FRET Basics and Applications: An EAMNET Teaching ...

14. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post

    ...interest will be expressed.      The RNA polymerase(s) RNA is transcribed from DNA using an RNA polymerase...

15. Negotiating Work and Life: How to Find the Joy

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    Blog Post

    ... not to do this. For a family with working parent(s), the sooner your kids can make a decent meal, fold...

16. Troubleshooting and Optimizing a Western Blot

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    Blog Post

    ...trouble by confirming your transfer worked with Ponceau S staining or other reversible protein staining immediately...resources References Luo, H., Rankin, G., Straley, S., & Chen, Y. (2011). Prolonged Incubation and Stacked...

17. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type

    Blog Post

    ...Foronda M, Zimmerman J, Diaz B, Zafra MP, Goswami S, Dow LE (2020) GO: a functional reporter system to...

18. Plasmids 101: Plasmid Incompatibility

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    Blog Post

    ...2958.2005.04643.x Pubmed PMID: 15916596 Kamruzzaman M, Shoma S, Thomas CM, Partridge SR, Iredell JR (2017) Plasmid...

19. Transferable Skills Guide: Creativity

    Type

    Blog Post

    ...mental-mistakes-that-kill-your-productivity Finkelstein, S. (2019, July 15). A brief exercise to spur innovation...

20. QC Sequencing Technologies at Addgene

    Type

    Blog Post

    ...//doi.org/10.1021/ac2010857  Sanger, F., Nicklen, S., & Coulson, A. R. (1977). DNA sequencing with chain-terminating...