We narrowed to 438 results for: %s

1. MXS Chaining

   Type

   Blog Post

   ...: PMC2822740. 2. Engler C, Kandzia R, Marillonnet S. A one pot, one step, precision cloning method with...

2. Plasmids 101: Gateway Cloning

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   Blog Post

   ...attL-entry-TOPO vector. TOPO cloning adds short end(s) to facilitate cloning into an attL-containing entry...

3. Isolating Genomic Regions of Interest with the CRISPR System

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   Blog Post

   ...(2017): 1028-1043. PubMed PMID: 28841410. Qi, Lei S., et al. "Repurposing CRISPR as an RNA-guided platform...

4. Plasmids 101: Codon usage bias

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   Blog Post

   ...well correlated with codon bias in both E. coli and S. cerevisiae. Protein folding - If a protein is encoded...

5. AAVs in Retinal Gene Therapy

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   Blog Post

   ...dystrophy)? AAV gene therapy started in the mid-90's with a lot of work and a little bit of luck A pioneer...

6. AAVs CREATed for Gene Delivery to the CNS and PNS

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   Blog Post

   ...the pre-designed primers to determine which version(s) of the capsid gene successfully infected the cell...

7. New Tools Enable CRISPRa for Neuroscience Applications

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   Blog Post

   ...lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (vector 1) with neuron-specific expression...

8. Bioinformatics at Addgene

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   Blog Post

   ...sequential computational tasks for which existing script(s) have been developed for a singular purpose (ex. adapter...

9. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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   Blog Post

   ... --very-sensitive-local -L 9 -N 1 -U GRNA_23MERS -S GRNA_HITS.sam Many thanks to our guest blogger, Aneesh...

10. Plasmids 101: Control Plasmids

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    Blog Post

    ...decreased expression of Gene X. Ergo the positive control(s) should decrease the expression of Gene X. Since the...

11. Tips for Using FRET in Your Experiments

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    Blog Post

    .... 2007 Sep; 32(9): 407-14. Zimmermann T,  Terjung S. FRET Basics and Applications: An EAMNET Teaching ...

12. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post

    ...interest will be expressed.      The RNA polymerase(s) RNA is transcribed from DNA using an RNA polymerase...

13. Negotiating Work and Life: How to Find the Joy

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    Blog Post

    ... not to do this. For a family with working parent(s), the sooner your kids can make a decent meal, fold...

14. Troubleshooting and Optimizing a Western Blot

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    Blog Post

    ...trouble by confirming your transfer worked with Ponceau S staining or other reversible protein staining immediately...resources References Luo, H., Rankin, G., Straley, S., & Chen, Y. (2011). Prolonged Incubation and Stacked...

15. QC Sequencing Technologies at Addgene

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    ...//doi.org/10.1021/ac2010857  Sanger, F., Nicklen, S., & Coulson, A. R. (1977). DNA sequencing with chain-terminating...

16. Hot Plasmids Spring 2024

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    Blog Post

    ...many closely-packed labeled cells. Plus, Voltron2’s fluorescence is tunable by changing the dye that becomes...

17. Transferable Skills Guide: Creativity

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    Blog Post

    ...mental-mistakes-that-kill-your-productivity Finkelstein, S. (2019, July 15). A brief exercise to spur innovation...

18. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    Blog Post

    ...Foronda M, Zimmerman J, Diaz B, Zafra MP, Goswami S, Dow LE (2020) GO: a functional reporter system to...

19. Plasmids 101: Plasmid Incompatibility

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    Blog Post

    ...2958.2005.04643.x Pubmed PMID: 15916596 Kamruzzaman M, Shoma S, Thomas CM, Partridge SR, Iredell JR (2017) Plasmid...

20. Changing Labor Laws Bring Increased Postdoc Wages

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    Blog Post

    ...Many thanks to our guest blogger Gary McDowell! Gary S. McDowell is currently the Executive Director of Future...