We narrowed to 575 results for: multi

1. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

   Type

   Blog Post

   ...Stop using EGFP/GFP for fusion proteins! Despite multiple studies in high profile journal articles, many...

2. Experimenting with New Careers while in Grad School

   Type

   Blog Post

   ...meet with various professionals, and there were multiple avenues for professional development including...

3. PITChing MMEJ as an Alternative Route for Gene Editing

   Type

   Blog Post

   ...Scalable Genome-Editing Based Approach for Mapping Multiprotein Complexes in Human Cells.” Cell Rep. 13: 621...

4. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

   Type

   Blog Post

   ...modular cloning system for standardized assembly of multigene constructs. Weber E, Engler C, Gruetzner R, Werner...

5. Tips for arabidopsis transformation

   Type

   Blog Post

   ...for transformants. It’s very important to select multiple T1 plants from each transformation. I like to ...

6. Capturing a Moment with Marc Zimmer’s “The State of Science”

   Type

   Blog Post

   ...certainly chapters that could warrant their own books. Multiple books have been written about CRISPR alone, and...

7. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

   Type

   Blog Post

   ...enough... If in the past you have tried to combine multiple terms in an Addgene plasmid search and found the...

8. Isolating Genomic Regions of Interest with the CRISPR System

   Type

   Blog Post

   ...targeting a specific genomic locus, which can require multiple cloning steps (Fujita et al., 2013). CRISPR-mediated...

9. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

   Type

   Blog Post

   ...you do need to work with dilute samples:  Take multiple readings to average out noisy measurements. Be...

10. Great Results Start with Great Standard Curves

    Type

    Blog Post

    ...measurement divided by the mean of all the replicates multiplied by 100. To calculate the %CV of the standard ...

11. Antibodies 101: Producing Recombinant Antibodies

    Type

    Blog Post

    ... the purity of your antibody prep. If you have multiple contaminating bands or a few strong ones, consider...

12. An Introduction to Adenovirus

    Type

    Blog Post

    ...., Hawkins, L. K., & Hermiston, T. W. (2003). Multigene expression from a replicating adenovirus using...

13. Getting to Know Expansion Microscopy

    Type

    Blog Post

    ...Introduction to Immunofluorescence  Antibodies 101: Multiplex Immunofluorescence Antibodies 101: Affinity Reagents...

14. CRISPR 101: Making a Knock-In Cell Line

    Type

    Blog Post

    ...PAM or sgRNA sequences Cas9 likely goes through multiple rounds of cleavage at the target site until either...

15. Droplet Digital PCR for AAV Quantitation

    Type

    Blog Post

    ...it is possible to measure the concentration of multiple targets simultaneously. For AAV titration, you...

16. Lentiviral Vector Uses and Overview

    Type

    Blog Post

    ...PMCID: PMC110254. 3. In-vivo RGB marking and multicolour single-cell tracking in the adult brain. Gomez-Nicola...

17. Plasmids 101: Colony PCR

    Type

    Blog Post

    ... mutations in your insert. Make sure to submit multiple positive clones for sequencing to verify the insert...

18. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type

    Blog Post

    ...mRNAs and minigenome. This can be accomplished in multiple ways: transfecting with an additional plasmid ...

19. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type

    Blog Post

    ...and PLoS One. Additionally, you are involved in multiple professional scientific societies. Why is being...

20. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type

    Blog Post

    ...and probably others) Neutral mutations    A multitude of applications Due to its size and ease of use...