1,189 results

A new open source tool for CRISPR experiments, CrispyCrunch, has been developed by the Chan Zuckerberg Initiative to design batch gRNAs, templates, and sequencing primers for CRISPR + HDR experiments.

Leaving the lab bench for a career outside of academic research can be a daunting task! In this blog post, we describe some ways to ease the transition from bench science to the office.

The tumor microenvironment is the cellular milieu that surrounds a tumor and contains immune cells, blood vessels, cells, and signaling molecules. What goes on in the cancer microenvironment affects and is affected and can either promote or repress tumor growth.

Aptamers are nucleic acid oligos that bind to small molecules to induce their fluorescence and can be used to study RNA localization and dynamics.

Stochastic multicolor labeling is a powerful tool for discriminating between neurons. Learn about how a new system named Tetbow (Tetracycline transactivator Brainbow) can bring brighter multicolor labeling to neuronal tracing experiments.

Fluorescent biosensors can be used to measuring changes in autophagic activity over time. Several autophagy biosensors, such as Rosella, Keima, and tandem fluorescent proteins are described.

Electronic lab notebooks (ELNs) are useful tools that help researchers keep track of data. However, not all ELNs support the same functionality when it comes to reproducibility.

The results from our #DeckTheLab social media contest are in! Read this blog post to see the Addgenie's favorite lab decorations.

Addgene celebrates 15 years of scientific sharing. In this post, executive director Joanne Kamens reflects on the past and future of Addgene.

Phage Directory is a directory of phage researchers and the phages they have in their labs with the goal of helping clinicians and scientists quickly get phages for phage therapy. Now, Phage Directory is also expanding to provide educational tools and resources to the phage community.

Addgene reached its 15 year anniversary on January 8, 2019. In this blog post, find the top 15 requested plasmids since our founding. Many of these include lentiviral or CRISPR tools!

Learn about CRISPR plant base editors for plants

The CRISPR babies saga shows us that we need action to move forward in bringing CRISPR technologies from bench to bedside.

This blog series showcases some of the new CRISPR tools we have at Addgene each quarter.

The Addgene viral team has found a way to increase AAV yields through media supplementation of sucrose, an endocytosis inhibitor.

If you're a scientist, you should use social media for networking and professional development! Read this blog post for some reasons why and five resources that can help you get started.

Learn all about how to use a new plasmid for RNAi in C. elegans, tools for proximity labeling, RNA-based methods for detecting viral outbreaks, and tools for characterizing protein domain binding specificities.

Cas14 was discovered by the Doudna lab. It cleaves ssDNA (but not dsDNA) in trans and can be adapted for a diagnostic toolkit such as DETECTR.

Seeding Labs and Addgene collaborate to provide scientific resources, equipment, and plasmids to researchers in resource-limited settings. Read more to learn about the recipients of the Addgene-Seeding Labs plasmid grant this year.

The purpose of this article is to explain what Unique Persistent Identifiers are, why they’re important, and to announce that Addgene now supports two different formats of plasmid identifiers and how they should be used.