We narrowed to 610 results for: sam
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TypeBlog Post... model organism in scientific research. Yes, the same kind we use in breads and other baked goods! Yeast...
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Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post...conclusive evidence that the fusion operates in the same way as the native protein. Although a fluorescent... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog Post...microtubule-severing protein, Katanin is of course named for the samurai sword called a katana. Opto-katanin consists of... -
CRISPR 101: Homology Directed Repair
TypeBlog Post... occur; however, if both HJs are resolved in the same manner, this results in a non-crossover event. ... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog Post...to the range of concentrations expected in your samples. Higher affinity isn’t always better, especially... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog Post..., and deep penetration of red-shifted light. The same NIR FPs and biosensors can be imaged at spatial ... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog Post...performing quantitative PCR titration of vector DNA in samples harvested 24 hours post-transduction (Wanisch and... -
Plasmids 101: Biotinylation
TypeBlog Post...pre-incubated with free avidin can be added to your sample. Any free biotin-binding sites on this complex ... -
Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog Post...require modifying multiple genes simultaneously in the same cell, are more appropriate for a CRISPR-based approach... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post...plasmids used to generate these fusions all use the same restriction enzymes for cloning. Furthermore, some... -
10 Steps to a Perfect Science Talk
TypeBlog Post... it perfectly fits the occasion. I speak on the same topics many times, but I always seek out information... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post...slightly different methods, they each observed the same positive phenotypes in cardiac and skeletal muscle... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post...underrepresented groups under their mentorship with the same critical eye they place on others within their labs... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post...three loxP variants, but the principles remain the same. The first fluorescent protein in the construct,... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog Post...convoluted due to researchers sharing and renaming samples throughout history. It was likely isolated in 1918... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...activate transcription. A lentiviral vector with the same modifier, Fuw-dCas9-Tet1CD, is available from Rudolf... -
Viral Vectors 101: Optogenetic Tools
TypeBlog Post...one cell type to another, as they may not use the same pathways. Additionally, as GPCR signaling is amplified... -
Hot Plasmids: Spring 2025
TypeBlog Post...proteoglycans composed of a protein core, 2–4 glycosaminoglycans (GAG), and are (typically) tethered to the... -
CRISPR 101: Off-Target Effects
TypeBlog Post...If you are performing CRISPR library screens, the same principle holds true - the higher the number of ... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog Post...They have successfully used guides targeting the same or complementary strands, as well as guides facing...