Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 610 results for: sam

Showing: 541 - 560 of 610 results

  16. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...population within a given tissue rather than a mixed sample. TRIBE was developed in Drosophila, and further...

  17. Chemogenetics Guide

    Type
    Guide
    ...arePharmacologically Selective Actuator Modules (PSAMs, pronounced SAMs). PSAMs are engineered α7 nicotinic acetylcholine...varenicline. This PSAM carries mutations at L131G, Q139L, and Y217F and is termed PSAM4 (or PSAM 4 ). Additional...cognate PSEM. For example, PSAM Q79G,Q139G is activated by PSEM 22S , while PSAM L141F,Y115F is activated... effort to develop a PSAM that responded to a clinically approved drug, the PSAM domain was engineered...have also been developed as agonists for PSAM4. A given PSAM is coupled with an ion pore domain (IPD) ...signaling. PSAM-based LGICs can be classified by the ion pore domain they are coupled to: PSAM-Gly LGICs...ions, and inhibits neuronal activity. PSAM-5HT3 LGICs pair a PSAM domain with a 5-HT 3 serotonin receptor...

  18. Plan Your Experiment

    Type
    Guide
    ...activator systems exist, including the multi-plasmid SAM system Activate Design and Clone Your gRNA Achieving...the genomic region you intend to target in your samples to ensure there are no discrepancies with the reference...

  19. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...empty NTC C AAV reference Sample 3 D E Sample 1 Sample 4 F E Sample 2 Sample 5 F Perform data analysis...most samples. If sample is expected to have a titer <1 x 10 12 GC/mL, use dilutions 3–6 If sample is expected...standards and samples in duplicate at least If possible, include an AAV reference sample of known titer...Count the number of samples (n) and prepare master mix for an additional 10 samples (n+10 – the additional...that there is enough master mix for all samples). Each sample requires 15 μL of master mix. Pro-Tips Use...pipetting error and sample evaporation. Make the master mix after all the samples have been added to the...virion). 5 μL sample + 39 μL H 2 O + 5 μL 10X DNase buffer + 1 μL DNase Gently mix sample (do not vortex...

  20. Centrifugation

    Type
    Protocol
    ...tube filled with water to the same volume as your samples. If your samples are significantly higher density...liquid sample. It achieves this by using centrifugal force, which is generated by spinning the sample at ... which you need to spin your samples. For example, spinning down samples in 1.5 mL microfuge tubes at ...the pellets form in approximately the same place in each sample. Place the centrifuge’s internal lid on...centrifuge to separate different components in a liquid sample. Protocols... containers, spin at different speeds, or keep samples at specific temperatures. This protocol will cover...for the biosafety level of the lab space Reagents Sample to be centrifuged Water Background Information ...
Showing: 541 - 560 of 610 results