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We narrowed to 644 results for: CAR

Showing: 41 - 60 of 644 results

  2. Transferable Skills: Negotiation

    Type
    Blog Post
    ... that I would mainly do over the price of a used car. Later, as I applied for jobs, I knew I should negotiate...Resources on Addgene.org Check out our careers page Watch our career videos  ...her data. Perhaps you both have an issue with childcare timing pickup. Whoever takes the earlier slot ...

  3. Hot Plasmids February 2024

    Type
    Blog Post
    ..., including in clinically-relevant lines such as CAR-T cells. Plus, this method is fast! These editing...

  4. Evolution of Lab Techniques

    Type
    Blog Post
    ... University. Just as computers, cell phones, and cars become more technologically advanced leaving earlier...

  5. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells. Cold Spring Harbor Laboratory, 17 Feb. 2024...https://doi.org/10.1186/s12896-017-0393-y. Engler, Carola, et al. “A Golden Gate Modular Cloning Toolbox ...

  6. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... Gadella 85053 pmScarlet-i_H2A_C1 Nucleus H2A mScarlet-I Dorus Gadella 85052 pmScarlet-H_H2A_C1 Nucleus...Nucleus H2A mScarlet-H Dorus Gadella 85051 pmScarlet_H2A_C1 Nucleus H2A mScarlet Dorus Gadella 18982 pHIV-...Bruchez 85065 pmScarlet-I_peroxisome_C1 Peroxisome SRL mScarlet-I Dorus Gadella 85064 pmScarlet-H_peroxisome_C1...Peroxisome SRL mScarlet-H Dorus Gadella 85063 pmScarlet_peroxisome_C1 Peroxisome SRL mScarlet Dorus Gadella...pBOB-CARMIL2 BH domain-GFP Plasma Membrane CARMIL2 BH domain GFP John Cooper 118737 pBOB-CARMIL2 BH domain-tdTomato...85045 pmScarlet_alphaTubulin_C1 Microtubules alpha-tubulin mScarlet Dorus Gadella 85046 pmScarlet-H_alphaTubulin_C1...alpha-tubulin mScarlet-H Dorus Gadella 85047 pmScarlet-i_alphaTubulin_C1 Microtubules alpha-tubulin mScarlet-I Dorus...

  7. Optogenetics AAV Preps

    Type
    Collection
    ...124650 pAAV-CamKIIa-C1V1(t/t)-mScarlet-KV2.1 CaMKII C1V1 (t/t) mScarlet Constitutive 9 Christopher Harvey...pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 CaMKII ChrimsonR (soma-targeted) mScarlet Constitutive 1, 9 Christopher...pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE CamKII ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive...-hSyn-ChRmine-mScarlet-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8...pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE EF1a ChRmine (high-photocurrent, red-shifted) mScarlet Cre dependent 1,...137158 pAAV-nEF-ChRmine-mScarlet nEF ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Karl...pAAV-nEF-Con/Fon-ChRmine-oScarlet nEF ChRmine (high-photocurrent, red-shifted) oScarlet Cre and Flp dependent...

  8. Serotype Testing AAV

    Type
    Collection
    ...Add to Cart AAV2 37825-AAV2.T 20 µL $ 150 Add to Cart AAV5 37825-AAV5.T 20 µL $ 150 Add to Cart AAV6 37825...Add to Cart AAV8 37825-AAV8.T 20 µL $ 150 Add to Cart AAV9 37825-AAV9.T 20 µL $ 150 Add to Cart AAV11 ...Add to Cart AAV2 50465-AAV2.T 20 µL $ 150 Add to Cart AAV5 50465-AAV5.T 20 µL $ 150 Add to Cart AAV8 50465...Add to Cart AAV9 50465-AAV9.T 20 µL $ 150 Add to Cart AAV11 50465-AAV11.T 20 µL $ 150 Add to Cart AAV Retrograde....T 20 µL $ 150 Add to Cart AAV Retrograde 37825-AAVrg.T 20 µL $ 150 Add to Cart pAAV-hSyn-EGFP (Plasmid... Retrograde 50465-AAVrg.T 20 µL $ 150 Add to Cart *Each viral service request will also include virus ...

  9. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression mScarlet 569 594 71 5.3 2.9 h Monomer pmScarlet_C1 - Mammalian Expression pCS2+mScarlet-C Cloning...Expression mCarmine 603 675 5.81 5.58 Monomer mCarmine in pcDNA3 - Mammalian Expression mCarmine in pRSETB...Maturation Structure Plasmids mScarlet-H 551 592 15 4.8 4.4 h Monomer pmScarlet-H_C1 - Mammalian Expression...mApple-pBAD - Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-I3_C1 - Mammalian Expression...pWXC078 - Worm Expression mScarlet3 569 592 78 4.5 37 min Monomer pmScarlet3_C1 - Mammalian Expression pWXC077...Vector - Mammalian Expression mScarlet-I 569 593 57 5.4 36 min Monomer pmScarlet-i_C1 - Mammalian Expression...Bacterial Expression mCardinal 604 659 17 5.3 27 min Prone to dimerization mCardinal-N1 - Mammalian Expression...

  10. Control AAV Preps

    Type
    Collection
    ...pAAV-CaMKIIa-mScarlet CamKIIa mScarlet Constitutive 8 Karl Deisseroth 131001 pAAV-hSyn-mScarlet Syn mScarlet... Jordane Dimidschstein 137135 pAAV-Ef1a-oScarlet EF1a oScarlet Constitutive 8 Karl Deisseroth 174112 pAAV-EF1a-mitoGFP-DIO...rg* Brandon Harvey 131002 pAAV-Ef1a-DIO mScarlet EF1a mScarlet Cre dependent 1, 5 Karl Deisseroth 50457...Deisseroth 137137 pAAV-Ef1a-Con/Foff 2.0-oScarlet EF1a oScarlet Cre dependent 8 Karl Deisseroth 137162 ...Karl Deisseroth 137138 pAAV-Ef1a-Coff/Fon-oScarlet EF1a oScarlet Flp dependent 8 Karl Deisseroth 55650 pAAV-hSyn...Karl Deisseroth 137136 pAAV-Ef1a-Con/Fon-oScarlet EF1a oScarlet Cre and Flp dependent 8 Karl Deisseroth...

  11. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ... Enzymes: SpeI , NotI , PstI Scar: TACTAGAG or TACTAG Features: 8 bp Scar; No In-Frame Fusions; Coding...Suffix Enzymes: Nhel , NotI , PstI Scar: GCTAGT Features: 6 bp scar encodes Ala-Ser; Allows for in-frame... CTCGAG Suffix Enzymes: BamHI , Xhol Scar: GGATCT Features: Scar encodes Gly-Ser in-frame with prefix ..., PstI Scar: ACTAGA Features: Modified RFC 10 with shortened prefix/suffix giving in-frame scar encoding... CTGCAG Suffix Enzymes: Agel , Spel , Notl, Pstl Scar: ACCGGC Features: Modified RFC 10 w/ start and stop...

  12. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Cre Yes 137135 pAAV-Ef1a-oScarlet None Yes 137136 pAAV-Ef1a-Con/Fon-oScarlet Cre AND Flp Yes 137137 pAAV-Ef1a-Con...pAAV-Ef1a-Con/Foff 2.0-oScarlet Cre AND NOT Flp FRT/F5 Yes 137138 pAAV-Ef1a-Coff/Fon-oScarlet Flp AND NOT Cre...137158 pAAV-nEF-ChRmine-mScarlet None Yes 137159 pAAV-nEF-Con/Fon-ChRmine-oScarlet Cre AND Flp Yes 137160...Fon-ChRmine-oScarlet Cre AND NOT Flp FRT/F5 Yes 137161 pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet Flp AND NOT...PubMed (Link opens in a new window) Poulin JF, Caronia G, Hofer C, Cui Q, Helm B, Ramakrishnan C, Chan...LE, Ramakrishnan C, Silberberg G, Deisseroth K, Carlén M, Meletis K. 2019. A hypothalamus-habenula circuit...

  13. Fluorescent Proteins: FRET

    Type
    Collection
    ...10 SYFP2 mScarlet3 515 0.68 592 104,000 0.75 6.3 4.8 pSYFP2-C1 , pmScarlet3_C1 , pDx_mScarlet3-SYFP2 mVenus...pLSSmOrange-mKate2 LSSmOrange* mScarlet3 437 0.45 592 104,000 0.75 5.8 4.3 pLSSmOrange-N1 , pmScarlet3_C1 mT-Sapphire* ...tdTomato-N1 CyOFP1* mCardinal 497 0.76 659 87,000 0.19 6.7 6.5 pNCS-CyOFP , mCardinal-N1 mAmetrine* tdTomato...and turquoise FRET pairs Gadella et al. (2023) mScarlet3: a brilliant and fast-maturing red fluorescent...

  14. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...118744 pScarlet-Kif5a KIF5A mScarlet CMV ALS Beverly Koller 118745 pCAG-Scarlet-Kif5a KIF5A mScarlet CAG ...pENTR221-VPS35-mScarlet VPS35 mScarlet Parkinson's Johanna Ivaska 215474 pEF.DEST51-VPS35-mScarlet VPS35 mScarlet...disease Caren Norden 105971 pME mKate2-dynactin1-811 DCTN1 mKate2 ALS, motor neuron disease Caren Norden...Arrowsmith 184825 pSN837 KIF5A mScarlet CMV ALS Kyoko Chiba 184826 pSN838 KIF5A mScarlet CMV ALS Kyoko Chiba 184827...184827 pSN526 KIF5A mScarlet Strep polH ALS Kyoko Chiba 184832 pSN840 KIF5A mScarlet Strep polH ALS Kyoko...JCE692) PRKCG PKC1 Spinocerebellar ataxia 16 Juan Carlos Igual 89925 Profiline PFN1 T7 ALS Guillaume Romet-Lemonne...Marius Wernig 176852 UBQLN2_450-624W UBQLN2 T7 ALS Carlos Castaneda 176915 pcDNA3.1(+)_Paragranulin+linker...

  15. Viral Vector Guides and Plasmids

    Type
    Collection
    ... the genetic cargo to specific cell types and tissues. Viral plasmids have been carefully engineered for...vectors rely on production plasmids that have been carefully optimized for both safety and efficiency. Learn...viruses are excellent vehicles to deliver genetic cargo into cells. To create safe and effective viral vectors... viral vectors that contain the desired genetic cargo. In turn, these viral vectors are used to transduce...

  16. CRISPR Guide

    Type
    Collection
    ...CRISPR plasmid will have all desired genomic edits carried out by Cas9. Multiplexing applications include ... and delivered to specific genomic locations to carry out unique tasks. We’ll discuss more Cas9 fusion...location in the genome, where the transposase then carries out integration of donor DNA. Both types of CASTs...with the sequence, so the edit is not considered “scarless”. Figure 7: Overview of CRISPR transposases. Browse...that displays improved specificity, and editors carrying an ADAR truncation that retain RNA editing capabilities... Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013)...Jaenisch, R. (2013). One-Step generation of mice carrying mutations in multiple genes by CRISPR/CAS-Mediated...

  17. NETRF

    Type
    Collection
    ...discover cures and more effective treatments for carcinoid, pancreatic, and related neuroendocrine cancers... Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou Harvard University Epigenetic... Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Bradley Bernstein Broad Institute Identifying...Altered Epigenetic States and Drivers in Intestinal Carcinoid and Pancreatic Neuroendocrine Tumors...

  18. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ... expert viral vector production team carefully review your cargo/serotype selection after you submit your...′ ITR exceeding 4.7 kb Transfer plasmids with a cargo that may reduce titer due to toxicity or other reasons...Disclaimer Due to the novel nature of experiments carried out with our materials, Addgene cannot guarantee...

  19. Plasmids for Stem Cell Research

    Type
    Collection
    ...Species Article PI iPSCs Cardiomyocytes Lentiviral Human Robust cardiomyocyte differentiation from human...reprogramming to cardiomyocytes using calcium activity as a functional measure of success. Mol Cell Cardiol. 2013...Sci U S A. 2012 May 29. Palecek Fibroblasts Cardiomyocytes Lentiviral Mouse Optimization of direct fibroblast...

  20. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Red calcium sensor PinkyCaMP based on mScarlet PinkyCaMP a mScarlet-based calcium sensor with exceptional... the sensor's behavior in the target system. Use care when applying a biosensor in a novel context or ...proteins enable metabolic assays at the point of care. Science. 2018 Sep 14;361(6407):1122-1126. Kai Johnsson...-regulatory role of the mitochondrial pyruvate carrier MPC. eLife. 2020 Mar 6;9. pii: 53917. Luis Felipe...sensor modules Grashoff Lab Tension Sensor Plasmids Carsten Grashoff Tension tCRMod tension sensors Tunable... voltage sensor to detect neural activity (Ace-mScarlet) A high-speed, bright, red fluorescent voltage...
Showing: 41 - 60 of 644 results