We narrowed to 453 results for: crispr

Overview of polycistronic glycine tRNA-gRNA (PTG) multiplexing gRNA cassettes, with comparisons to Pol III and Cys4 gRNA cassettes.

This blog post describes a a self-excising cassette that can be used with CRISPR/Cas9 tools in C. elegans to generate genomic modifications.

An all-in-one CRISPR/Cas9 Multiplex Genome Engineering System for transfecting and editing cell lines.

Emmanuelle Charpentier and Jennifer Doudna awarded for their CRISPR/Cas9 research at the 2014 Johnson & Johnson's Dr. Paul Janssen Award dinner.

A collection of Addgene's video content, including how-to screen captures, lab procedure protocols, and career advice videos.

In this post we directly compare the Argonaute genome editing protein, NgAgo, to the CRISPR endonuclease, Cas9.

The labs of John Doench and David Root have recently created CRISPR libraries to knock out, inactivate, or activate genes for genome-wide screens. These optimized libraries are effective for probing gene function while using fewer sgRNAs.

Learn how to verify indels, deletions, and homology directed repair (HDR) events in your CRISPR modified cell lines.

Overview of the CRISPRi system for rewiring metabolic circuitry, with a focus on the paper by Zalatan et al 2015.

New CRISPR pooled libraries, such as SAM, CRISPRa, and CRISPRi, offer new ways to do genome-wide functional screens.

Learn about optogenetics tools, methods for increasing CRISPR editing efficiency, CRISPR base editors, and DIY DNA ladders.

An overview of cytosine and adenine base transition editors, which make precise changes in DNA bases without causing double-strand breaks.

In this episode of the Addgene Podcast, learn all about research in Singapore and the genome engineering tools being developed in Wei Leong Chew's Lab.

Learn about B. subtilis libraries, iPSC reprogramming tools, TZAP, and a plant CRISPR toolkit.

Learn all about CAPTURE - a new CRISPR-based technique to study regulatory elements in their native chromatin contexts.

Addgene interviews Hodaka Fujii of Osaka University to learn about his lab's enCHIP system, their new CRISPR tools, community involvement, and more.

An updated look at how CRISPR is being used in therapeudic applications across a variety of diseases.

Twelve new CRISPR tools and resources are highlighted in 2022's 12 Days of CRISPR.

Enjoy 12 new CRISPR articles from Addgene's 12 Days of CRISPR!

Antibiotic resistant bacteria are a huge issue in the clinic - learn how CRISPR is being developed to fight against antibiotic resistant super bugs.