We narrowed to 213 results for: grna

Learn about the latest CRISPR plasmids deposited at Addgene including plasmids for CRISPRi in Burkholderi, DNA and RNA editing, epitope tagging, and Cas9/gRNA ribonucleoprotein delivery.

Learn about new plasmids at Addgene including plasmids for CRISPR, sparse cell labeling, reporting calcineurin activity, and an alternative to DNA staining dyes.

Resources to help improve precision genome editing through homology directed repair in experiments with CRISPR nucleases.

A paper published in Nature used CRISPR to correct a disease-causing mutation in human embryos. Does this paper herald a future of human germline editing?

If you’re using CRISPR to make a genome edit, how do you know if your experiment was successful? Try using fluorescent CRISPR reporters like SRIRACCHA and GEmCherry2.

The Addgene repository has many amazing plasmids used by labs all over the world, but what plasmid was request most in 2015?

Rationally engineered Cas9 variants eSpCas9 SpCas9-HF1 enhance CRISPR on-target specificity. Learn how in this post.

Golden gate cloning can be used to efficiently and inexpensively clone multiple inserts into a single vector. Learn the basics of golden gate here.

A review of alternative PAM and PAM-flexible Cas enzymes.

Genome-wide CRISPR/Cas9 screens are a high-throughput systematic approach for identifying genes involved in a biological process. Recently the Bassik Lab at Stanford developed a new genome-wide CRISPR knockout screen to analyze unanswered questions about CRISPR screen design by including safe targeting guides and short gRNAs.

Learn the basics behind generating generating mouse models using CRISPR/Cas9.

A new open source tool for CRISPR experiments, CrispyCrunch, has been developed by the Chan Zuckerberg Initiative to design batch gRNAs, templates, and sequencing primers for CRISPR + HDR experiments.

SapTrap improves CRISPR genomic tagging in C. elegans by offering scientists an easy one tube assembly reaction to generate high-throughput targeting vectors.

Check out our updated CRISPR web resources and learn more about the applications of your favorite genome editing tool.

This blog post explores how CRISPR/Cas9 can be used to visualize multiple intrachromosomal loci in vivo and map the 4D nucleome.

Learn how to design a CRISPR screening experiment targeting enhancers.

Cpf1, a newly discovered Cas9 homolog, is smaller than Cas9, can target different sequences, and may more efficiently generate targeted mutations.

An all-in-one CRISPR/Cas9 Multiplex Genome Engineering System for transfecting and editing cell lines.

An easy demethylation protocol uses CRISPR-based dCas9-directed DNA avoids the use of Tet

The development of adenoviral vectors for the delivery of CRISPR/Cas9 expands genome engineering to primary cells.