We narrowed to 68 results for: phage

A review of alternative PAM and PAM-flexible Cas enzymes.

Plasmid-encoded antibodies offers many advantages over other types of antibodies. Learn about how recombinant antibodies are made.

This round-up of new plasmids from Addgene include blue-light inducible Cre for use in bacteria, CRISPR-Casɸ mGold, and new CRISPR plasmids and viral preps.

Joanne Kamens provides highlights from the 2015 Canton Nucleic Acids Forum (CNAF).

Read on for a sampling of some of the exciting new plasmid technologies that have come through our doors in 2015.

Protocol that can be used to titer lentiviral preparations carrying a fluorescence marker.

There are many plasmid tools available in molecular biology so how do you chose the right cloning method for you? Here, consider speed, cost, difficulty, efficiency, and the number of inserts when designing your cloning strategy.

Having trouble with your transductions? Find troubleshooting tips from Addgene scientist Leila Haery.

Two new CRISPR tools from the Abudayyeh-Gootenberg lab are now available at Addgene: Cas7/11 and PASTE.

Learn how microrganisms develop antibiotic resistance and how you can take some simple steps to combat them.

An overview of cytosine and adenine base transition editors, which make precise changes in DNA bases without causing double-strand breaks.

Prime editing is a search-and-replace CRISPR editing method that offers versatility and precision in genome editing. We cover common prime editing tools.

Browse neurodegeneration plasmids for research related to Alzheimer's Disease, ALS, Frontotemporal Dementia, Huntington's Disease, Parkinson's Disease, and others.

Use this protocol to generate lentivirus

This protocol can be used to titer lentiviral preparations that confer antibiotic resistance.

Addgene lentiviral packaging, envelope, and transfer plasmids

Baby KJ was treated for his devastating genetic disorder with CRISPR base editing. This post describes the prior advances that made his cure possible.

Joining the popular sacB counterselection method is a new suite of toxin-based allelic exchange vector that can be used in many proteobacterial genomes.

Learn about Cre-Lox and other site-specific recombinase systems, how to use them to modify the genome or control gene expression, and find plasmids for your experiments.

Overview of split vector approaches for fitting genes >4.7 kB into AAV including overlapping, transplicing, hybrid, and sequential homologous recombination.