We narrowed to 67 results for: phage

... hospitals. Dr. Qimron proposes using bacterial phage vectors with CRISPR/cas9 sensitizing gene cassettes...

...2018). xCas9 was generated by the Liu lab through phage-assisted evolution, specifically with the goal of...

...antibodies. The selection process is often performed via phage display, yeast display, ribosome display, or mammalian...

...genome editor from the Biggiephage clade of huge phages. In this article, they investigated three Casɸ ...

...method, developed by Invitrogen and based on lambda phage integration sequences, relies on site-specific recombination...

...cells were transduced with a range of dilutions of pHAGE-TO-dCas9-3XmCherry . 72 h post transduction, cells...

...Synthetic Biology Center have deposited a set of 11 phage integrases which act irreversibly on their cognate.... These pairs are closely related to the lambda phage integrase system which is the basis for Gateway ...authors recently used computational protein design, phage display and high throughput binding assays, to engineer... great specificity and resolution by using 6-12 phage PP7 coat protein binding sites and 24 copies of ...

...Central PMCID: PMC3760253. 2. Rohwer, Forest. "Global phage diversity." Cell 113.2 (2003): 141. PubMed PMID:...

... good at flipping. So the lab instead turned to phage serine integrases, where they saw 10-20% integration...

...Antibiotic Resistance Genes Are Used in the Lab Probe Phage Genomes for Host Binding Proteins Learn How to Use...

...and specificity. In this first, the Liu lab used phage-assisted evolution selection systems to generate...Bauer, D. E., Doudna, J. A., & Liu, D. R. (2020). Phage-assisted evolution of an adenine base editor with...

...μL total per 10 cm dish). Using transfer plasmid pHAGE TRE dCas9-KRAB (total μg of plasmid DNA 27.8 μg)...

...indicated serial dilutions of the lentiviral vector pHAGE EF1α dCas9-KRAB . The following day, cells were ...

...Edward Fon 116364 pHAGE-ERBB4-K606N ERBB4 EF-1 alpha ALS Kenneth Scott 116365 pHAGE-ERBB4-S774G ERBB4 ...MacGillavry 131791 pHAGE-N-FLAG-HA-TBK1 TBK1 Flag, HA CMV ALS Alicia Pickrell 131792 pHAGE-TBK1-C-FLAG-HA ...ALS Claudia Kutter 175750 pHAGE-eGFP-OPTN OPTN ALS Wade Harper 175760 pHAGE-FLAG-APEX2-OPTN OPTN Flag ...Wade Harper 175762 pHAGE-eGFP-OPTN D474N OPTN GFP CMV ALS Wade Harper 175763 pHAGE-eGFP-OPTN S473A 513A...Wade Harper 175764 pHAGE-eGFP-OPTN S513A OPTN GFP CMV ALS Wade Harper 175765 pHAGE-eGFP-OPTN E50K OPTN...Ching Cheong 208866 pHAGE-FKBP-GFP-OPTN OPTN FKBP, GFP CMV ALS Sascha Martens 208867 pHAGE-FKBP-GFP-OPTN (...ERBB4 EF-1 alpha ALS Kenneth Scott 116736 pHAGE-ERBB4 ERBB4 EF-1 alpha ALS Kenneth Scott 117701 pmSod1-WT-...

...tetracycline transactivator. Rudolf Jaenisch 131626 pHAGE-mt-mKeima 3rd Expression of mitochondrial mKeima...

...Bauer, D. E., Doudna, J. A., & Liu, D. R. (2020). Phage-assisted evolution of an adenine base editor with...

...effector toxins, toxin-antitoxin type toxins, and phage holins. They hypothesized that genes that had evolved...

...hydroxamate uptake, iron uptake receptor mutation. T1/T5 Phage resistance gal Mutation in galactose metabolism...

.... PhiC31 integrase, derived from a Streptomyces phage, and Bxb1 integrase, from a mycobacteriophage, are...

... homology sequence derived from the filamentous phage F1 (Trapani et al., 2014). Drawbacks: The choice...