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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,052 results for: sta

Showing: 41 - 60 of 1052 results

  1. Immunology Research Plasmids and Resources

    Type
    Collection
    ...MGC110940, OPN SST somatostatin SMST SSTR1 somatostatin receptor 1 SRIF-2 SSTR2 somatostatin receptor 2 - SSTR5...immunoglobulin heavy constant alpha 2 (A2m marker) - IGHD immunoglobulin heavy constant delta FLJ00382, FLJ46727...IGHE immunoglobulin heavy constant epsilon IgE IGHG1 immunoglobulin heavy constant gamma 1 (G1m marker) -...immunoglobulin heavy constant gamma 2 (G2m marker) DKFZp686I04196 IGHG3 immunoglobulin heavy constant gamma 3 (G3m...immunoglobulin lambda constant 1 (Mcg marker) IGLC IGLC2 immunoglobulin lambda constant 2 (Kern-Oz- marker...immunoglobulin lambda constant 3 (Kern-Oz+ marker) IGLC IGLC6 immunoglobulin lambda constant 6 (Kern+Oz- marker...early stage tumors ARP, MANF, MGC142148, MGC142150 ARMETL1 arginine-rich, mutated in early stage tumors-like...

  2. CRISPR Guide

    Type
    Collection
    ...used to install the LSR Bxb1’s landing site into the genome at a desired locus, then Bxb1 installs the donor...including resistances to chemotherapy drugs and toxins, cell viability, and tumor metastasis. One of the...genome-wide screens. This guide will provide a basic understanding of CRISPR biology, introduce the various applications...applications of CRISPR, and help you get started using CRISPR in your own research. CRISPR Basics In this...target cleavage, while mismatches toward the 5′ end distal to the PAM often permit target cleavage. Generating... Cas9 orthologs are available. SaCas9 (from Staphylococcus aureus ) has a coding sequence ~1 kb shorter...addition of a structured motif to enhance pegRNA stability PEmax - optimized PE architecture; increased synergy...

  3. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...telangiectasia Michael Kastan 32300 hATMS1981A ATM His, Flag CMV Ataxia telangiectasia Michael Kastan 32813 CMV-hEAAT1...'s Alexander Stark 71083 pFOSGE_CUS1A07 GRN Frontotemporal dementia (FTD) Alexander Stark 71433 pmGFP10C..., FTD Stefan Stamm 203366 Tau BA 12->7 I260V MAPT Flag CMV Parkinson's, FTD Stefan Stamm 203367 Tau BA..., FTD Stefan Stamm 203368 Tau BA 12->7 G272V MAPT Flag CMV Parkinson's, FTD Stefan Stamm 203371 Tau BA..., FTD Stefan Stamm 203372 Tau BA 12->7 P301S MAPT Flag CMV Parkinson's, FTD Stefan Stamm 203373 Tau BA..., FTD Stefan Stamm 203374 Tau BA 12->7 S305N MAPT Flag CMV Parkinson's, FTD Stefan Stamm 203375 Tau BA..., FTD Stefan Stamm 203376 Tau BA 12->7 K317M MAPT Flag CMV Parkinson's, FTD Stefan Stamm 203378 Tau BA...

  4. AAV for Neuronal Tracing

    Type
    Collection
    ...is provided to the starter cell in trans , via delivery by an AAV. Since this starter cell now has all ...TVA and G-protein is delivered to the starter cell. (2) The starter cell now expresses TVA and G-protein... RAVB is delivered to the starter cell, and specifically targets the starter cell via the EnvA receptor...). Thus, G-deleted rabies can infect a single "starter" cell, but new virus cannot spread to any neighboring...can spread to cells that are projecting to the starter cell). Once in the secondarily infected cells that... the targeting of G-deleted rabies to specific starter cells, the tropism of this virus was modified so...receptor. RABV is able to replicate in the starter cell using the G-protein, which is supplied in trans . (...

  5. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    ... panels) equipped with destabilized GFP calcineurin reporter (blue) and stained with PI (pink) to indicate...Image from Isaacman-Beck et al., 2019. Get started with sparse labeling Isaacman-Beck et al., bioRxiv...bioRxiv 2019. https://doi.org/10.1101/788679 A destabilized GFP reporter for calcineurin activity in yeast...transcriptional reporter consisting of a quickly-degraded destabilized GFP variant fused to a 4X CDRE repeat. In addition... that this method is compatible with dead cell staining using propidium iodine (PI), allowing for calcineurin...Truncated TALE-fluorescent protein fusions as a DNA staining dye Article contributed by Jennifer Tsang ...generated fluorescent protein fusions with tTALE to “stain” DNA. TALE proteins can bind specific DNA sequences...

  6. Plan Your Experiment

    Type
    Collection
    ...Once you have a clear understanding of your experimental goal, you are ready to start designing the specific...This page provides a general framework to get you started using CRISPR in your research and setting up CRISPR...aware that there could be downstream translation start sites that could allow for expression of a truncated...elongation. CRISPR Activation : Target the transcription start site. CRISPRa requires dCas9 to be fused to transcriptional...to be within a given range of the transcription start site in order to function effectively. Select gRNAs...into plasmids containing the gRNA scaffold using standard restriction-ligation cloning. However, the exact...experiment) and the expression time (transient or stable). For plasmid and viral-based approaches, the species...

  7. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Mouse IgG2a 114557 Anti-TRIP8b (constant) [N269/73R] TRIP8b (constant) Rat Mouse IgG2a 114558 Anti-Synaptotagmin...Mouse IgG2a 128635 Anti-Ankyrin-G (staining) [N106/43R] Ankyrin-G (staining) Human Mouse IgG2a 128636 Anti-LRRTM4...Rat Mouse IgG2a 140065 Anti-SCG10/Stathmin-2 [L5/1R] SCG10/Stathmin-2 Rat Mouse IgG2a 140066 Anti-Co-Rest...-Pan-Gamma-protocadherin-Constant [N159/5R] Pan-Gamma-protocadherin-Constant Mouse IgG2a 177504 Anti-LAR... Mouse IgG2a 177514 Anti-TRIP8b (constant) [N212/7R] TRIP8b (constant) Rat Mouse IgG2a 177515 Anti-RBM17...Mouse IgG2a 177521 Anti-TARPGamma2/Stargazin [N245/1R] TARPGamma2/Stargazin Rat Mouse IgG2a 177522 Anti-TARPGamma2...Mouse IgG2b 206664 Anti-TRIP8b (constant) [N212/17R-2b] TRIP8b (constant) Rat Mouse IgG2b 206665 Anti-SynCAM4...

  8. Antibody Production

    Type
    Collection
    ...concentration is confirmed by Coomassie staining. For Coomassie staining, the sample is separated via a denaturing...alongside serial dilutions of a standard antibody of known concentration and stained with Coomassie blue. Protein...and a standard curve is generated. The final antibody concentration is calculated from the standard curve...by Addgene scientists. They are generated using standard methods individually optimized for each specific...opens in a new window) page. Storage and Shipping Stability Addgene does not recommend repeated freeze thaw... antibody catalog item is tested for shipping stability by incubating at 37 °C for 2 weeks, freezing at...recommended assays. If an antibody has passed stability testing, it may ship at room temperature. However...

  9. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...transduction of the eye and other tissues. As part of our standard viral production , these viral vectors undergo...The following serotypes were developed in Arun Srivastava’s or Shannon Boye’s lab at the University of Florida...derived from AAV2 capsid and developed by Arun Srivastava’s lab. AAV2(trpYF) The AAV2(trpYF) serotype shows...The AAV2(trpYF) serotype was developed by Arun Srivastava’s lab. It is derived from the AAV2 capsid and ...AAV6(dbY-F+T-V) serotype was developed by Arun Srivastava’s lab. It is derived from the AAV6 capsid and ... future publications, please acknowledge Arun Srivastava and cite Petrs-Silva, et al. 2009. High-efficiency... future publications, please acknowledge Arun Srivastava and cite Petrs-Silva, et al. 2011. Novel properties...

  10. Viral Production

    Type
    Collection
    ...electrophoresis (PAGE) followed by silver staining or SYPRO Ruby staining and the molecular weight and relative...after negative staining. Empty vector particles can be identified after negative staining and appear darker...of Viral Production Viruses are generated using standard methods that have been optimized for each specific... 2–3 fold higher than those achieved using the standard qPCR method. Titering by the University of Pennsylvania...high quality viral vectors by optimizing and standardizing production protocols and performing rigorous...purity of the AAV preparation. Figure 1: Silver staining of purified and non-purified AAV subjected to ...production are propagated in the endA -mutated NEB Stable strain of E. coli . In addition, plasmids are typically...

  11. Luciferase Plasmid Collection

    Type
    Collection
    ...Structurally destabilized firefly luciferases : A set of three increasingly destabilized mutants which... signal, sensitivity, wide dynamic range, and resistance to photobleaching have made luciferase a popular...luminescence. Gaussia luciferase is secreted and more stable, enabling time course experiments, but is too dim... luciferase mutant Rluc8, which has increased stability and light output Sanjiv Sam Gambhir 104587 pHIV-iRFP720...luciferases with altered emission, substrate use, stability, or other features. AkaLuc : Engineered to use...protein production. Other Sensors and Tools Optimized STARR-seq (Self-Transcribing Active Regulatory Region)...reporter. These plasmids improve on the original STARR-seq by using the bacterial ORI as the core promoter...

  12. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... cytosolic NADH/NAD+ redox state Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded ...calcium sensor with exceptional brightness, photostability, and multiplexing capabilities. bioRxiv. 2025...FRET sensors to monitor intracellular Zn(2+) homeostasis. Nat Methods. 2009 Oct;6(10):737-40. Maarten ...and ZapCY2, Golgi or ER targeted Measuring steady-state and dynamic endoplasmic reticulum and Golgi Zn2+...localized zinc sensor ZIBG2 Genetically Encoded, Photostable Indicators to Image Dynamic Zn(2+) Secretion ...sensor of ATP:ADP ratio (PercevalHR) Imaging energy status in live cells with a fluorescent biosensor of the...sensors for cAMP feature exceptional brightness, photostability and dynamic range: characterization of dedicated...

  13. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...-iCAP-PHP.eB PHP.eB AAV packaging plasmid (non-standard), expressing Rep2 and PHP.eB Cap controlled by...pUCmini-iCAP-PHP.S PHP.S AAV packaging plasmid (non-standard), expressing Rep2 and PHP.S Cap controlled by ...-iCAP-PHP.V1 PHP.V1 AAV packaging plasmid (non-standard), expressing Rep2 and PHP.V1 Cap controlled by...pUCmini-iCAP-AAV.CAP-B10 B10 AAV packaging plasmid (non-standard), expressing Rep2 and B10 Cap controlled by tTA-TRE...pUCmini-iCAP-AAV.CAP-B22 B22 AAV packaging plasmid (non-standard), expressing Rep2 and B22 Cap controlled by tTA-TRE...pUCmini-iCAP-AAV.MaCPNS1 MaCPNS1 AAV packaging plasmid (non-standard), expressing Rep2 and MaCPNS1 Cap controlled by...pUCmini-iCAP-AAV.MaCPNS2 MaCPNS2 AAV packaging plasmid (non-standard), expressing Rep2 and MaCPNS2 Cap controlled by...

  14. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... included a function section for if you want to stably express an insert using viral vectors, are studying...attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker Also see more empty backbones on our Plant...proteins are excellent tools for further understanding the function of your favorite gene. For example...the stop codon for C-terminal tags and omit the start codon for N-terminal tags. And when you are designing...experiments, scientists often want to create stable cell lines in which the expression cassette of ...Variant of lentiCRISPR v2 that confers hygromycin resistance pRGEB32 - Express sgRNA/PTG with rice snoRNA ...loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker pLenti CMV/TO GFP-Zeo DEST (719-1) - 3rd...

  15. Lentivirus Plasmids

    Type
    Collection
    ...shRNA empty plasmid with puro resistance. See plasmid 24150 for hygro resistance. Weinberg 10878 pLKO.1 – ...significant immune response. These viruses also integrate stably into the host genome, enabling long term transgene...Wiederschain 11578 pSico 3rd Conditional (Cre-lox), stable expression of shRNAs; addition of Cre turns on ...fusion; can be used for cDNA expression; puro resistance Sabatini 14883 FUGW 3rd hUbC-driven EGFP; can...expression, gateway plasmid, constitutive Hygromycin resistance gene coexpression. See article for other versions...neomycin selection. See plasmid 21915 for Puro resistance. Wiederschain 36084 pLV-mCherry 3rd CMV driven...

  16. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...their promising research projects.   Understanding drug resistance mechanisms of Mycobacterium tuberculosis...interested in understanding the mechanisms behind Mycobacterium tuberculosis resistance to pyrazinamide...targets. An early and accurate detection of PZA-resistance is necessary to improve tuberculosis care and...Sheen aims to identify new genes related to PZA-resistance by using different approaches, including CRISPR...Her research should provide us with a better understanding of PZA action and could potentially stimulate...diagnostic methods and improved therapies for PZA-resistant Mycobacteria tuberculosis. Investigation of multispecies...this condition are urgently needed. Therefore, understanding neuronal networks, their interactions and information...

  17. Synthetic Biology - Overview

    Type
    Collection
    ...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering SynBio Depositing...Michel Maharbiz Thorsten Mascher Kathleen Matthews Anastasios Melis Aindrila Mukhopadhyay Richard Murray Erin...Sauro Claudia Schmidt-Dannert Pamela Silver Lei Stanley Qi Jeff Tabor Jan-Willem Veening Christopher Voigt...community. To deposit, view our SynBio Deposit Guide or start our online deposit process . Browse SynBio Plasmids...Genetically Engineered Machine Foundation Registry of Standard Biological Parts - Repository of community-created...Collection - Open access papers on Syn Bio SEVA - Standard European Vector Architecture Open Plant - Plant...

  18. Zebrafish Plasmid Collection

    Type
    Collection
    ...system that allows sustained cell loss and ablation of previously resistant cell types. CreLite... fish in the minnow family. It has long been established as a powerful vertebrate model organism for the...high-throughput screening and non-invasive live imaging. A standard toolbox of genetic methods, such as Tol2 transposons...CRISPR/Cas9-based gene editing make the zebrafish a stalwart research model. With more than 70% of human disease...Fusion Construct Set - Amro Hamdoun Lab. A useful “starter kit” for screening fluorescent protein fusion expression...

  19. Rett Syndrome

    Type
    Collection
    ... and regression of speech. In contrast to the sustained loss of skills in neurodegenerative conditions...self-limited, after which a period of developmental stabilization or even limited recovery of skills occurs. Diagnosis...in mouse and rat models have accelerated our understanding of Rett syndrome at the molecular level. They...Knock-in of NLuc-tdTomato at endogenous MECP2 locus Castaneus MECP2-NLuc-tdTomato mouse reporter cell line (...syndrome-specific differentiated cells that can be used to understand the mechanisms of the disease and for the development...resource by contributing your plasmids! To get started with your deposit, please click here ....

  20. Retrovirus Plasmids

    Type
    Collection
    ...cells; additional plasmids include Puro resistance and Neo resistance Weinberg 10676 pMKO.1 GFP MoMLV U6-driven...immortalized cells (see Weinberg Lab page for resistance variants) Weinberg 1773 pBABE-hygro-hTERT MoMLV... also expresses GFP. See pMKO.1 puro for Puro resistance. Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline...lncRNA expression plasmid Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase in mammalian cells Wong...
Showing: 41 - 60 of 1052 results