Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 1,046 results for: sta

Showing: 41 - 60 of 1046 results

  1. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...packaged strains, which are shipped as bacterial stabs at room temperature. Individual plasmids can be ...each plasmid page and will be shipped as bacterial stabs. Add to Cart $ 385 USD + shipping Available to academics...Contents The following items will be shipped as stabs at room temperature. ID Name 13421 pAC-Kan-alphaGal4...

  2. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ... director for more information. Why MDH? MDH is stable, inexpensive to assay, and easy to purify using...institutions. With these plasmids, faculty can begin a sustainable MDH-related CURE focusing on protein expression...Link opens in a new window) — Learn how to get started with a CURE, explore MDH projects, find teaching...

  3. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...luciferase with neomycin resistance. Christopher Vakoc 138152 pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro...-MCS-Hygro Lentiviral vector expressing destabilized EGFP. Neville Sanjana 164432 pHAGE-CMV-Luc2-IRES-...

  4. mTOR Pathway

    Type
    Collection
    ...Ras Pathway p53 Pathway Tackling Cancers’ Drug Resistance with a New Screening Kit Background mTOR Pathway...integrates these signals to determine the nutritional state of the cell and regulates the switch from anabolic... Kit (Sabatini & Wood) Tackling Cancers’ Drug Resistance with a New Screening Kit (from David Sabatini...

  5. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...72258 Expresses FLPe, copGFP and the puromycin resistance from the CB promoter pCDH-CB-FLPe-P2A-tdTomato...copGFP-T2A-Puro 72263 Express copGFP and the puromycin resistance gene from the EF1 promoter pCDH-CMV-mCherry-T2A-Puro...-T2A-Puro 72264 Express mCherry and puromycin resistance gene from the CMV promoter pCDH-CMV 72265 Express...

  6. Open Enzyme Collection

    Type
    Collection
    ...polymerase 165501 pOpen-GBDpol Pyrococcus sp. Heat-Stable DNA Polymerase 165502 pOpen-9N7polA (CT) 9 degrees...P45 165572 pOpen-GBDpol (exo) Pyrococcus sp. Heat-Stable (exo-) DNA Polymerase 165573 pOpen-phi29pol phi29...DNA-binding protein 165549 pOpen-ETSSB Extreme Thermostable Single-Stranded DNA-Binding Protein 165579 pOpen-dromedNK...

  7. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Tribolium castaneum BsaI none S. pyogenes Averof p(U6b-BsaI-gRNA) 65956 Other/Tribolium castaneum BsaI none...Hygro Yang pBUE411 62200 Plant yes, cut S. pyogenes Basta Chen pKSE401 62202 Plant yes, cut S. pyogenes Kan...Kim pBAtC 78097 Plant AarI yes, cut S. pyogenes Basta Kim pMZ283 52224 Yeast CspCI none S. pyogenes URA4...

  8. COVID-19 Resources

    Type
    Collection
    ...community moved quickly to expand the knowledge and understanding of COVID-19 and related coronaviruses. Addgene...cleaves precursor proteins to a biologically active state. The SARS-CoV-2 S protein contains a potential cleavage...Mutagenesis Libraries Screening Jesse Bloom Tyler Starr Libraries of variant Spike protein RBD mutations...

  9. Lentivirus Plasmids

    Type
    Collection
    ...significant immune response. These viruses also integrate stably into the host genome, enabling long-term transgene...Wiederschain 11578 pSico 3rd Conditional (Cre-lox), stable expression of shRNAs; addition of Cre turns on ...EGFP 3rd CMV-driven EGFP fusion with puromycin resistance. Can be used for cDNA expression. David Sabatini...

  10. Neurodegeneration Research Collection

    Type
    Collection
    ...barrier integrity, and using CRISPR screens to understand more globally how neurons function. They are ...and death of dopamine-producing neurons in the substantia nigra, one of the movement control centers in...more. Study ER structure and function with siRNA-resistance SigmaR1 constructs . (Sawyer et al. Dev Cell....

  11. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...sequences corresponding to every previously observed instance of human serine phosphorylation ( Rinehart Human...in need of reference spectra or phosphopeptide standards for quantitative assessments. The Mode #1 phosphosite... Mode #1 Library Plasmid 69118 E17TAG GFP zeo resistance Plasmid 68307 SupD Strain 68306 C321.ΔA.Δserb.Amp...

  12. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...scientific research ecosystem, not just in the United States but around the world. As an organization that directly...research infrastructure that has taken decades to establish.These funding decisions will have far-reaching consequences...progress depends on our collective response. Addgene stands ready to collaborate with partners across the research...will directly shape our response initiatives as we stand with the scientific community. Let us know by emailing...

  13. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...choose to start their own academic research laboratory. How does one even go about starting a lab? Obviously...students and postdocs when starting out. She advises “do not hurry to staff up the lab and to take things...resources for hiring research staff. And for a more indepth resource for starting your academic lab, download...a PI without a personal assistant has to deal with. My inbox currently stands at about 65,000 messages...for advice on staffing your lab. Let us know if you have any relevant tips for starting a lab. Or share...effective working environment? Tom Ellis was offered a start-up package that included a postdoc and a PhD student...important to me to be with them in the lab when they started, so that we could discuss our projects and ideas...

  14. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ... you. Statistics – The basics Statistical analysis is what determines if there is statistical significance... several core factors of statistics relevant for many downstream statistical tests.   Independent variables...are statistically different. They are classic parametric tests and one of the simplest statistical tests...described above. Violated assumptions and statistics Statistical power, the ability to correctly identify...is a better option. Stay tuned for our upcoming blog on non-parametric statistical tests! Adding it all...your friendly neighborhood biostatistician happy by making your statistical analysis plan when you design...Wissing, Dennis R., and Timm, Donna. Statistics for the nonstatistician: Part I. South Med J., 105(3): 126...

  15. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...was GeoCas9 discovered? The discovery of GeoCas9 started with an interesting question: why engineer a new...active at high temperatures Figure 1: Thermostability of SpCas9 vs GeoCas9. SpCas9 or GeoCas9 RNPs...GeoCas9’s editing activity in HEK293’s. GeoCas9 stability in human plasma The last trick GeoCas9 has up ...Harrington et al suspected GeoCas9 might have higher stability since prior research had shown that a cocaine ...cocaine esterase engineered to have increased thermostability also had a longer lifetime in human blood. To ...and cleavage product analyzed on a gel. SpCas9 is stable when incubated in reaction buffer alone, but has...lower than optimal temperatures. GeoCas9’s thermostability could also be used with in vitro molecular ...

  16. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...Biomedical research, for instance, contributes heavily to medical practice, and understanding the scientific process...can vary "Lab assistants" usually wash glassware, prep media, etc. "Research assistants" are more involved... maintenance Administrative assistant: assists PI with admin duties Staff scientist: similar role to a...professors, and other mentors, and four years after I started college, I will be entering a PhD program to continue... As scientists, we probe beyond the realm of established theory. Realizing that textbooks don’t tell us... process can help us understand how new therapies can be developed and applied. The current pandemic serves...the lab Official title is typically Professor, Assistant Professor, Associate Professor Sometimes teaches...

  17. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ... creation of the United States Environmental Protection Agency, which started making regulations such ... the second in a series of posts on bringing sustainable practices in the lab. The first post focused ...learn about some creative approaches to lab sustainability, and find ways to further reduce our consumption...consumption beyond recycling.     Choosing more sustainable products Recycling is great for rerouting waste... grant agencies), we can shift toward a more sustainable one. The math on this is pretty simple: wash ...solutions and practices to meet higher environmental standards. Find some easy action items on our other article...

  18. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...location where the United States Postal Service picks up mail. NEB actually started this program in 1976! ..., flip the flaps of the box so the pre-paid postage stamp is on the outside of the container, and drop...off the box at any location where the United States Postal Service picks up mail. Recycle plastic packaging...more lab sustainability tips, check out our other articles in this series: Bringing Sustainable Practices...popularity of single-use items, plastic is a substantial portion of lab waste. Despite the fact that researchers...cost of regular waste services. Learn about how to start a glove recycling program at your institute! What...culture room for the wrappers and ship them back. Start a glove recycling program at your institute! Universities...

  19. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...involved, it can be difficult for scientists to understand how GPCR activation leads to downstream effects... the endogenous ligand or drug. In the inactive state, the luminescent chimeric protein Gα-Rluc8 and Gγ-GFP2...resulting in a decreased GFP2 emission. The activation state can be measured by a BRET2 ratio, which is the ratio...pharmaceutical drugs, there is still only a partial understanding of how GPCRs signal through G protein signal...provides scientists with a toolkit to gain an understanding of the signaling through the transducerome from...as well as the activation by drugs to further understand their mechanism. These plasmids are available... the transducerome, to gain a more complete understanding of a GPCR mediated response.  Acknowledgements...

  20. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...can use the loss of resistance as a marker for successful cloning. In these instances, the cloning vector...Resistance to antibiotics is a widely used tool in molecular biology, yet scientists rarely stop to think...to maintain the plasmid. Adding an antibiotic resistance gene to the plasmid solves both problems at once...pressure to keep your plasmid. Viva la (bacterial) resistance!  What are antibiotics? Antibiotics are generally...bactericidal) or slow growth/prevent cell division (bacteriostatic); however, the distinction between the two ...categories may be a bit of a gray area as some bacteriostatic reagents can kill bacteria when used at high...subunit; inhibits aminoacyl translocation Bacteriostatic 50-100 ug/mL in EtOH Polymyxin B polypeptide...
Showing: 41 - 60 of 1046 results