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We narrowed to 808 results for: eng

Showing: 581 - 600 of 808 results

  3. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ... perfect for molecular cloning and metabolic engineering.  The E. coli genome has an extensive research...you are working with Arabidopsis check out the GreenGate Toolkit which allows for easy cloning of constructs...

  4. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...sites? Never fear! You can introduce a silent, passenger edit to serve as a proxy for your edit of interest... a version of Cas9 that has been specifically engineered for reduced off target editing, such as SpCas9...

  5. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...(see Figure 2). H describes the maximum signal strength, while W results from the time that the cell spent...increase the electric current and thereby the signal strength. So, we need to adjust the voltage in the cytometer...

  6. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...used here could be an attractive strategy for engineering other enzymes. Conclusion We hope this post has...precise control over vascular endothelial barrier strength. eLife, 12, RP84364. https://doi.org/10.7554/eLife...

  7. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...photoreceptors (Oliinyk et al., 2019). They were engineered to incorporate BV. Their advantages are their...Verkhusha, V.V. Near-infrared fluorescent proteins engineered from bacterial phytochromes. Curr Opin Chem Biol...

  8. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...validation PCR followed by Restriction Fragment Length Polymorphism (RFLP) analysis (right). Introduction... absence) of that particular site with fragment length polymorphism (RFLP) analysis. In this process, ...

  10. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...important and abundant neurotransmitters, but it is challenging to monitor because only a small number of glutamate...to specific cell types in the brain can be a challenging — but critical — factor for successful experiments...

  12. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ..., Pillay, S., Puschnik, A. S., Nagamine, C. M., Cheng, F., Qiu, J., Carette, J. E., & Vandenberghe, L..... E., & Manfredsson, F. P. (2017). Rationally Engineered AAV Capsids Improve Transduction and Volumetric...

  13. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...are currently about 5.5 million articles in the English version of Wikipedia alone. Humans have been forced...a team is a really good way of creating and strengthening those social bonds. So never mind how much better...

  14. Antibodies 101: Validation

    Type
    Blog Post
    ... signal, so you’ll need a range of expression strengths to be confident in your data. This is a good option...9. https://doi.org/10.1038/518027a  Roncador G, Engel P, Maestre L, Anderson AP, Cordell JL, Cragg MS,...

  15. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...Sleeping Beauty, piggyBac and Tol2 for Genome Engineering. IJMS 22:5084. https://doi.org/10.3390/ijms22105084...about the INTEGRATE system for bacterial genome engineering  Read about the Sleeping Beauty DNA transposon...

  19. CRISPR Guide Update

    Type
    Blog Post
    ...the Cascade-Cas3 system. We also describe newly engineered Cas9s with more flexible requirements and enhanced...
Showing: 581 - 600 of 808 results