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We narrowed to 803 results for: eng

Showing: 581 - 600 of 803 results

  1. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...called Drosha to pre-miRNA. miRNA plasmids are engineered to skip this first pri-miRNA step. These RNA ...1472-6750-6-1 O’Brien, J., Hayder, H., Zayed, Y., & Peng, C. (2018). Overview of MicroRNA biogenesis, mechanisms...

  2. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...(see Figure 2). H describes the maximum signal strength, while W results from the time that the cell spent...increase the electric current and thereby the signal strength. So, we need to adjust the voltage in the cytometer...

  3. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...sites? Never fear! You can introduce a silent, passenger edit to serve as a proxy for your edit of interest... a version of Cas9 that has been specifically engineered for reduced off target editing, such as SpCas9...

  4. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...validation PCR followed by Restriction Fragment Length Polymorphism (RFLP) analysis (right). Introduction... absence) of that particular site with fragment length polymorphism (RFLP) analysis. In this process, ...

  6. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ..., Pillay, S., Puschnik, A. S., Nagamine, C. M., Cheng, F., Qiu, J., Carette, J. E., & Vandenberghe, L..... E., & Manfredsson, F. P. (2017). Rationally Engineered AAV Capsids Improve Transduction and Volumetric...

  7. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...important and abundant neurotransmitters, but it is challenging to monitor because only a small number of glutamate...to specific cell types in the brain can be a challenging — but critical — factor for successful experiments...

  8. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...on. In addition, researchers have created many engineered serotypes by mixing, matching, and modifying ...summarize briefly: AAVrg is derived from AAV2 and engineered to infect neurons and undergo retrograde transport...

  10. Antibodies 101: Validation

    Type
    Blog Post
    ... signal, so you’ll need a range of expression strengths to be confident in your data. This is a good option...9. https://doi.org/10.1038/518027a  Roncador G, Engel P, Maestre L, Anderson AP, Cordell JL, Cragg MS,...

  11. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...Sleeping Beauty, piggyBac and Tol2 for Genome Engineering. IJMS 22:5084. https://doi.org/10.3390/ijms22105084...about the INTEGRATE system for bacterial genome engineering  Read about the Sleeping Beauty DNA transposon...

  12. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...are currently about 5.5 million articles in the English version of Wikipedia alone. Humans have been forced...a team is a really good way of creating and strengthening those social bonds. So never mind how much better...

  16. CRISPR Guide Update

    Type
    Blog Post
    ...the Cascade-Cas3 system. We also describe newly engineered Cas9s with more flexible requirements and enhanced...

  18. The 12 Days of CRISPR

    Type
    Blog Post
    ...the 1st day of CRISPR, Addgene gave to me: An engineered prime editor with enhanced editing efficiency...
Showing: 581 - 600 of 803 results