...meetings. When scientists tell good stories, they engage their readers or listeners thus expanding the impact...invite people to ask questions, be approachable. Engage with your audience and try to learn from them. ... again until you work out what people enjoy and engage with and what parts you can leave out. Start viewing...

...sharing cells: the black market of cell biology. Engaging in this practice would seem to save time and money...

... is becoming ever more urgent for scientists to engage broadly with diverse communities, to raise awareness...and concerns of these communities. This kind of engagement and two-way dialogue is crucial if we as a society...law enforcement, continues to hamper efforts to engage these communities, including those by researchers...worked with a number of organizations in public engagement efforts, including giving public lectures, writing...takes on a variety of projects. With our focus on engaging the community and facilitating dialogue, a major...your field of research will allow you to better engage these diverse audiences and to identify issues ...ethical level, scientists arguably have a duty to engage the public on the implications of their work. ...

...tools, producing lentivirus, can pose certain challenges. Whether choosing a system that is the best fit...will provide an overview of some of the common challenges associated with producing and using lentivirus...make these optimization experiments a bit more challenging. With these considerations in mind, when planning...

...well-characterized genetic tools make it challenging to engineer a specific function in these multicellular...ve had to navigate the challenges involved in plant synthetic biology. Challenge #1: Public perception ...facilitates plant science. Challenge #3: Intellectual property There is another challenge associated with molecular...and Keira Havens, co-founders of Revolution Bioengineering. Read their previous blog post about how they... company here. The first transgenic plant was engineered over 30 years ago, but plant synthetic biology...fibers. And because working with plants can be challenging, there are a lot of unexplored areas in plant...directed at GM crops has burdened this emerging engineering technology. Monoculture and chemical spraying...

...Your task is to find them, scavenger hunt style. 🔎 Here’s how the scavenger hunt works works: You will...correct photo, you will see a reference to this scavenger hunt and the subsequent clue. (Begin with clue...until April 7, 2021 to make your way through the scavenger hunt and enter the raffle. We encourage you to...menu and search tool to help you navigate the scavenger hunt. Good luck and have fun exploring the blog...

...California, San Diego. The revolution in genetic engineering techniques is a speculation of yesteryear which... of the authors during this period.  Genetic Engineering Era: Pre-CRISPR  Having visualized and understood...devise methods to manipulate it. The term genetic engineering itself is attributed to SciFi writer Jack Williamson...s Island. In 1976, the world’s first genetic engineering company, Genentech, was founded.  This commercialization...these corporations would extrapolate genetic engineering to exploit human beings. Ridley Scott directed...humans called replicants which are genetically engineered by a powerful corporation to serve as slaves....with the DNA of present-day frogs via genetic engineering. The corresponding movie came out in 1993 and...

...posts 3 Challenges in Plant Synthetic Biology Quick, Versatile Plant Transgenesis with GreenGate Plasmids...the plants that we grow and eat, and genetic engineering will continue to improve plant nutrition, yield..., and pest resistance. Much of plant genetic engineering revolves around Agrobacterium tumifaciens. Agrobacterium...with biolistic methods, making it useful for engineering plants that are not susceptible to Agrobacterium...have tested the system with genes up to 4 kb in length and confirmed successful protein expression via...customizability, pSiM24 can be used not only for plant engineering and biotechnology applications, but also for ... plant genetic engineering...

...these defenses. For some applications of genome engineering - such as certain forms of gene therapy - avoiding...tool and an alternative to CRISPRs for genome engineering whose use is only likely to grow in the coming...  genome engineering...

...going virtual for Addgene’s first AAV Data Hub Challenge, now taking place Dec. 6, 2022! Share your AAV...

...plate technique. Time for the #BioSci3319 #StreakPlateChallenge part 1. Lab B needs a redo :-( @SandTBioSci... the lab used three different species for the challenge, but one species always outcompeted the other ...it would be a perfect fit for the streak plate challenge. Chromoproteins are a subset of the fluorescent... distinguish colonies from one another in the challenge. To create a successful streak plate, first the...colonies in the last quadrant after incubation. The challenge is usually successful for the students. “They ...colonies. I should probably make it a bit more challenging, potentially by using a thicker bacterial suspension...transformation efficiency.  Aside from the Streak Plate Challenge, Westenberg’s course includes Winogradsky columns...

...Health (NIH) Swiss. Genetically engineered mice Mice can be engineered by inserting a targeting vector... successfully engineered cells. Similar to transgenic mice, knockout mice are engineered during the embryonic...to understand mammalian traits and genetically engineered mouse models provide researchers with useful ...models, several common techniques used to create engineered mouse models at embryonic stages, and the pros...or treatments, but are not useful for genetic engineering. Examples of outbred strains include Swiss Webster...blastocyst. There are several types of genetically engineered mice: transgenic mice, knockout mice, and mice...DNA breaks, such as ZFN and TALEN, can used to engineer knockin mice. Co-injection of a targeting vector...

.... To tackle the PAM problem, researchers have engineered alternative Cas9s binding distinct PAM sequences... and NNNNGATT, respectively. Researchers also engineered SpCas9 variants with alternative PAMs like NGAG...transcriptional regulation and base editing Hu et al. also engineered transcriptional activators using the xCas9 scaffold...Additional Resources on the Addgene Blog Learn about Engineered Cas9 PAM Variants Read about CRISPR Base Editing...

...that this basic concept of gRNA design is still challenging for researchers using CRISPR, and that standardizing...paraphrased in the tweet below. The complexity and challenges of homology directed repair (HDR) are echoed ...s a list of blog posts to help you tackle the challenges of DNA repair (with bonus gRNA design resources... HDR repair. Check out this post to see if an engineered Cas9 variant might work for your experiment. ...Creating knock-in mouse models with CRISPR has been challenging, with protocols using dsDNA templates achieving...

...brain at a level of abstraction that enables the engineering of its function." Once scientists can do that...big questions that motivate his interest in neuroengineering, and some of the optogenetic tools that are...are still missing. Optogenetic tools for neuroengineering Addgene: For those who may not be experts, can... neuroengineering...

...inexpensive enzymes. Now, a new toolkit - aptly named GreenGate – offers all of those same advantages to researchers...advanced molecular plant science lab.” Introducing GreenGate As Lohmann and his colleagues describe in the ...ONE paper that introduced the kit in December, GreenGate includes the six most frequently used elements... selection of transgenic plants. The complete GreenGate toolkit, now available at Addgene, also gives ...transgenic plants. In the most practical terms, what GreenGate can do is “save you half a year,” in comparison...Lohmann said. The key component of Golden Gate and GreenGate are type IIS restriction endonucleases. They are... in a single tube. (Additional details on the GreenGate kit and how it works are available here.) Although...

...).  CRISPR-transposons for bacterial genomes engineering In the Sternberg group’s initial report of VchINT...additions to the growing CRISPR toolbox for genome engineering in bacteria, alongside more established tools...currently studies CRISPR biology and CRISPR genome engineering technologies. Message or follow him on twitter...high-efficiency, multiplexed bacterial genome engineering. Nat Biotechnol. https://doi.org/10.1038/s41587...

...built our new search engine atop Elasticsearch, a powerful open-source search engine that can scale to search...users who prefer to use search, our updated search engine is now more powerful, intuitive, and searches across...team identified. The basic function of a search engine is to find the website resources that best match...in order to more granularly customize the search engine, and a scoping mechanism that allows category-specific...category to expose as filters and designed the search engine to return only the filters that are relevant to...information for a given resource, and designed the search engine to weight search results differently according ...more technical details that make our new search engine unique. Most importantly, with this new implementation...

... an engineering school as an undergraduate, I never fooled myself into thinking I was an engineer. The...talk, which focused on the history of genome engineering from ZFNs through TALENs and CRISPR-Cas nucleases...names in the CRISPR-Cas9 field – Jennifer Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! ...huge impact on this field. Advances in genome engineering & CRISPR technology That afternoon's workshop...workshop, entitled "Advances in Genome Engineering", brought us 8 short talks on every conceivable way of examining...systems, modifications to the RNA components, and challenging target genomes, such as the malaria-causing Plasmodium...Addgene depositor, discussed some of the many challenges of creating biological circuits, and I can only...

...enzymes are the latest tools in the transcriptome engineering toolbox. How were Cas13d enzymes discovered?...property is size - Cas13d enzymes have a median length of ~930aa - making them 17-26% smaller than other... tell how large the genome and transcriptome engineering toolbox will be. It is, however, certain that...References Konermann, Silvana, et al. “Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors...