We narrowed to 212 results for: FAST

1. CasPEDIA: A Functional Classification of Cas Enzymes

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   Blog Post

   ...available and applied by researchers for gene targeting. Fast forward a decade, and the CRISPR field has exploded...

2. Early Career Researcher Toolbox: Social Media for Scientists

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   Blog Post

   ...on Al Jazeera and NPR, articles in magazines like Fast Company, opportunities to speak at conferences, ...

3. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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   Blog Post

   ...frequently used for PL were APEX2 and BioID. APEX2 is fast, tagging proximal proteins within minutes, but requires...Nucleic Acids Research. 2018 PubMed PMID: 29924347 Faster proximity labeling with TurboID and miniTurbo Article...

4. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   Blog Post

   ...throughput and faster. It’s also easy to run things in parallel. Addgene: Besides being fast and efficient...

5. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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   Blog Post

   ...market, and (C) Break even cycles. Work hard. Work fast. Be flexible. Be quick at failing and succeeding...

6. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

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   Blog Post

   ...-based MAGE The Nielsen lab’s CRMAGE system is a fast, multiplexable method that combines CRISPR and recombineering-based...possible with efficiency >70%. CRMAGE is an incredibly fast protocol, with only 5 hours incubation time needed...

7. Antibodies 101: Fab Fragments

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   Blog Post

   ...Kiuchi T, Takagi J, Watanabe N (2022) Engineered fast-dissociating antibody fragments for multiplexed ...

8. Viral Vectors 101: An Introduction to AAV

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   Blog Post

   ...the same way the host DNA does. Because of this, fast dividing tissues will lose expression quicker than...

9. Hot Plasmids and Viral Preps - March 2021

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   Blog Post

   ...available from Tonia Rex's lab.  vfCRISPR, or very fast CRISPR, is a light-inducible CRISPR system developed...

10. RNA Extraction Without A Kit

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    Blog Post

    ...is degraded. To effectively isolate intact RNA, a fast, strong protein denaturant would be required- something...

11. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post

    ...contains. Biotech equipment becomes obsolete amazingly fast, but with a wide range of skills among our members...

12. Plasmids 101: Protein Expression

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    Blog Post

    ...acids. Cell-free expression systems are ideal for fast assembly of more than one protein in one reaction...

13. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    Blog Post

    ...amino acid peptide, the CRY2clust domain enables fast and reversible oligomerization when fused to a protein...termination of light stimulation. CRY2clust enables faster assembly and disassembly dynamics, higher sensitivity...optogenetic system (OptoSTIM1), caused two-fold faster changes in intracellular Ca2+ levels than the original...

14. CRISPR in the Clinic

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    Blog Post

    ...as well as improving delivery strategies to make fast, effective, and targeted therapy possible. This ...

15. Tips for Technical Support Calls

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    Blog Post

    ...one. Though an initial response should be fairly fast, getting the full answer to a complicated question...responses asking for more specifics. We can help you faster if we get as much information as possible about...

16. Adapting Toehold Switches to Detect Zika Virus

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    Blog Post

    ...declared a global health emergency and called for the fast-tracked development of diagnostics. Mostly transmitted...

17. Plasmids 101: Optimizing Plasmid Yields

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    Blog Post

    ...volume and you should ensure the shaker speed is fast enough for sufficient gas exchange in the overnight...especially those in deep-well plates, require a faster shaking speed for proper aeration (260-300 RPM)...

18. Technical Design of a Western Blot

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    Blog Post

    ...various manufacturers. These proprietary systems are fast (often <10 minutes) and simple, utilizing individually...fluorescent proteins. Chemiluminescence enzymes are cheap, fast, shelf-stable before activation, and light-insensitive...primary antibodies). Direct westerns are simpler and faster than indirect ones and can be used in highly quantitative...for very large proteins. A semi-dry transfer is faster — 3–30 minutes — and uses less buffer, but it is...

19. 10 Basic tips for mammalian cell culture

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    Blog Post

    ...form a routine. Twice a week often works for most fast-growing cell lines (such as HEK293, which multiplies...

20. Viral Vectors 101: Chemogenetics

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    Blog Post

    ...specific neuron firing are better equipped using fast-acting optogenetic tools. The advantages provided...